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Glucosidase 2 subunit beta (PRKCSH) (NM_002743) Human Tagged ORF Clone
CAT＃: RC213274
- TrueORF®
PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1
ORF Plasmid: tGFP
"NM_002743" in other vectors (4)
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CNY 6,288.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGE-R2; G19P1; GIIB; PCLD; PCLD1; PKCSH; PLD1; VASAP-60 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213274 representing NM_002743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTGCCGCTGCTGCTGCTGCTACCCATGTGCTGGGCCGTGGAGGTCAAGAGGCCCCGGGGCGTCT CCCTCACCAATCATCACTTCTACGATGAGTCCAAGCCTTTCACCTGCCTGGACGGTTCGGCCACCATCCC ATTTGATCAGGTCAACGATGACTATTGCGACTGCAAAGATGGCTCTGACGAGCCAGGCACGGCTGCCTGT CCTAATGGCAGCTTCCACTGCACCAACACTGGCTATAAGCCCCTGTATATCCCCTCCAACCGGGTCAACG ATGGTGTTTGTGACTGCTGCGATGGAACAGACGAGTACAACAGCGGCGTCATCTGTGAGAACACCTGCAA AGAGAAGGGCCGTAAGGAGAGAGAGTCCCTGCAGCAGATGGCCGAGGTCACCCGCGAAGGGTTCCGTCTG AAGAAGATCCTTATTGAGGACTGGAAGAAGGCACGGGAGGAGAAGCAGAAAAAGCTCATTGAGCTACAGG CTGGGAAGAAGTCTCTGGAAGACCAGGTGGAGATGCTGCGGACAGTGAAGGAGGAAGCTGAGAAGCCAGA GAGAGAGGCCAAAGAGCAGCACCAGAAGCTGTGGGAAGAGCAGCTGGCTGCTGCCAAGGCCCAACAGGAG CAGGAGCTGGCGGCTGATGCCTTCAAGGAGCTGGATGATGACATGGACGGGACGGTCTCGGTGACTGAGC TGCAGACTCACCCGGAGCTGGACACAGATGGGGATGGGGCGTTGTCAGAAGCGGAAGCTCAGGCCCTCCT CAGTGGGGACACACAGACAGACGCCACCTCTTTCTACGACCGCGTCTGGGCCGCCATCAGGGACAAGTAC CGGTCCGAGGCACTGCCCACCGACCTTCCAGCACCTTCTGCCCCTGACTTGACGGAGCCCAAGGAGGAGC AGCCGCCAGTGCCCTCGTCGCCCACAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAGGCTGA AGAAGAGGAGGAGGAGGAGGATTCCGAGGAGGCCCCACCGCCACTGTCACCCCCGCAGCCGGCCAGCCCT GCTGAGGAAGACAAAATGCCGCCCTACGACGAGCAGACGCAGGCCTTCATCGATGCTGCCCAGGAGGCCC GCAACAAGTTCGAGGAGGCCGAGCGGTCGCTGAAGGACATGGAGGAGTCCATCAGGAACCTGGAGCAAGA GATTTCTTTTGACTTTGGCCCCAACGGGGAGTTTGCTTACCTGTACAGCCAGTGCTACGAGCTCACCACC AACGAATACGTCTACCGCCTCTGCCCCTTCAAGCTTGTCTCGCAGAAACCCAAACTCGGGGGCTCTCCCA CCAGCCTTGGCACCTGGGGCTCATGGATTGGCCCCGACCACGACAAGTTCAGTGCCATGAAGTATGAGCA AGGCACGGGCTGCTGGCAGGGCCCCAACCGCTCCACCACCGTGCGCCTCCTGTGCGGGAAAGAGACCATG GTGACCAGCACCACAGAGCCCAGTCGCTGCGAGTACCTCATGGAGCTGATGACGCCAGCCGCCTGCCCGG AGCCACCGCCTGAAGCACCCACCGAAGACGACCATGACGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213274 representing NM_002743
Red=Cloning site Green=Tags(s) MLLPLLLLLPMCWAVEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAAC PNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRL KKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQE QELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKY RSEALPTDLPAPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASP AEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTT NEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETM VTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002743 |
| ORF Size | 1584 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002743.3 |
| RefSeq Size | 2262 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 5589 |
| UniProt ID | P14314 |
| Protein Families | Druggable Genome |
| MW | 59.2 kDa |
| Gene Summary | This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213274L3 | Lenti-ORF clone of PRKCSH (Myc-DDK-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 6,460.00 |
|
| RC213274L4 | Lenti-ORF clone of PRKCSH (mGFP-tagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 6,460.00 |
|
| RG213274 | PRKCSH (tGFP-tagged) - Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 7,888.00 |
|
| SC118458 | PRKCSH (untagged)-Human protein kinase C substrate 80K-H (PRKCSH), transcript variant 1 |
CNY 5,904.00 |
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