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ASD3 (MYH6) (NM_002471) Human Tagged ORF Clone
CAT＃: RC213673
- TrueORF®
MYH6 (Myc-DDK-tagged)-Human myosin, heavy chain 6, cardiac muscle, alpha (MYH6)
ORF Plasmid: tGFP
"NM_002471" in other vectors (3)
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CNY 19,552.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | alpha-MHC; ASD3; CMD1EE; CMH14; MYHC; MYHCA; SSS3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213673 representing NM_002471
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGATGCCCAGATGGCTGACTTTGGGGCAGCGGCCCAGTACCTCCGCAAGTCAGAGAAGGAGCGTC TAGAGGCCCAGACCCGGCCCTTTGACATTCGCACTGAGTGCTTCGTGCCCGATGACAAGGAAGAGTTTGT CAAAGCCAAGATTTTGTCCCGGGAGGGAGGCAAGGTCATTGCTGAAACCGAGAATGGGAAGACGGTGACT GTGAAGGAGGACCAGGTGTTGCAGCAGAACCCACCCAAGTTCGACAAGATTGAGGACATGGCCATGCTGA CCTTCCTGCACGAGCCCGCGGTGCTTTTCAACCTCAAGGAGCGCTACGCGGCCTGGATGATATATACCTA CTCGGGCCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCGGTGTACAATGCCGAGGTGGTGGCC GCCTACCGGGGCAAGAAGAGGAGTGAGGCCCCGCCCCACATCTTCTCCATCTCCGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACGGGAGAATCCGGGGCGGGGAAGACTGTGAA CACCAAGCGTGTCATCCAGTACTTTGCCAGCATTGCAGCCATAGGTGACCGTGGCAAGAAGGACAATGCC AATGCGAACAAGGGCACCCTGGAGGACCAGATCATCCAGGCCAACCCCGCTCTGGAGGCCTTCGGCAATG CCAAGACTGTCCGGAACGACAACTCCTCCCGCTTTGGGAAATTCATTAGGATCCACTTTGGGGCCACTGG AAAGCTGGCTTCTGCAGACATAGAGACCTACCTGCTGGAGAAGTCCCGGGTGATCTTCCAGCTGAAAGCT GAGAGAAACTACCACATCTTCTACCAGATTCTGTCCAACAAGAAGCCGGAGTTGCTGGACATGCTGCTGG TCACCAACAATCCCTACGACTACGCCTTCGTGTCTCAGGGAGAGGTGTCCGTGGCCTCCATTGATGACTC CGAGGAGCTCATGGCCACCGATAGTGCCTTTGACGTGCTGGGCTTCACTTCAGAGGAGAAAGCTGGCGTC TACAAGCTGACGGGAGCCATCATGCACTACGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CGGAGCCAGACGGCACCGAAGATGCTGACAAGTCGGCCTACCTCATGGGGCTGAACTCAGCTGACCTGCT CAAGGGGCTGTGCCACCCTCGGGTGAAAGTGGGCAACGAGTATGTCACCAAGGGGCAGAGCGTGCAGCAG GTGTACTACTCCATCGGGGCTCTGGCCAAGGCAGTGTATGAGAAGATGTTCAACTGGATGGTGACGCGCA TCAACGCCACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGAGTCCTGGACATCGCTGGCTTCGA GATCTTCGACTTCAACAGCTTTGAGCAGCTCTGCATCAACTTCACCAACGAGAAGCTGCAGCAGTTCTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGACATTCATTGACT TTGGCATGGACCTGCAGGCCTGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGA GGAGTGCATGTTCCCCAAGGCCACTGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAG TCCAACAATTTCCAGAAGCCACGCAACATCAAGGGGAAGCAGGAAGCCCACTTCTCCCTGATCCACTACG CCGGCACTGTGGACTACAACATCCTGGGCTGGCTGGAAAAAAACAAGGATCCTCTCAACGAGACTGTTGT GGCCCTGTACCAGAAGTCCTCCCTCAAGCTCATGGCCACTCTCTTCTCCTCCTACGCAACTGCCGATACT GGGGACAGTGGTAAAAGCAAAGGAGGCAAGAAAAAGGGCTCATCCTTCCAGACGGTGTCGGCTCTCCACC GGGAAAATCTCAACAAGCTAATGACCAACCTGAGGACCACCCATCCTCACTTTGTGCGTTGCATCATCCC CAATGAGCGGAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGCTGCAATGGCGTG CTGGAGGGCATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATCCTCTACGGGGACTTCCGGCAGAGGT ATCGCATCCTGAACCCAGTGGCCATCCCTGAGGGACAGTTCATTGATAGCAGGAAGGGGACAGAGAAGCT GCTCAGCTCTCTGGACATTGATCACAACCAGTACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCAGGG CTGCTTGGGCTGCTGGAGGAGATGCGGGATGAGAGGCTGAGCCGCATCATCACGCGCATGCAGGCCCAAG CCCGGGGCCAGCTCATGCGCATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTAATCCA GTGGAACATTCGGGCCTTCATGGGGGTCAAGAATTGGCCCTGGATGAAGCTCTACTTCAAGATCAAGCCG CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCACCATGAAGGAAGAGTTCGGGCGCATCAAAGAGA CGCTGGAGAAGTCCGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA TGACCTGCAGCTCCAAGTGCAGGCGGAACAAGACAACCTCAATGATGCTGAGGAGCGCTGCGACCAGCTG ATCAAAAACAAGATTCAGCTGGAGGCCAAAGTAAAGGAGATGAATGAGAGGCTGGAGGATGAGGAGGAGA TGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGACGAGTGCTCAGAGCTCAAGAAGGACATTGA TGACCTGGAGCTGACACTGGCCAAGGTGGAGAAGGAGAAGCATGCAACAGAGAACAAGGTGAAGAACCTA ACAGAGGAGATGGCTGGGCTGGATGAAATCATCGCTAAGCTGACCAAGGAGAAGAAAGCTCTACAAGAGG CCCATCAGCAGGCCCTGGATGACCTTCAGGTTGAGGAAGACAAGGTCAACAGCCTGTCCAAGTCTAAGGT CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTAGAGCAAGAGAAGAAGGTGCGCATGGACCTG GAGCGAGCAAAGCGGAAACTGGAGGGCGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAAAATG ATAAACTGCAGCTGGAAGAAAAGCTTAAGAAGAAGGAGTTTGACATTAATCAGCAGAACAGTAAGATTGA GGATGAGCAGGTGCTGGCCCTTCAACTACAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG GAGGAGGAGCTGGAGGCCGAGCGCACCGCCAGGGCTAAGGTGGAGAAGCTGCGCTCAGACCTGTCTCGGG AGCTGGAGGAGATCAGCGAGCGGCTGGAAGAGGCCGGCGGGGCCACGTCCGTGCAGATCGAGATGAACAA GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACT GCCGCGGCCCTGCGCAAGAAGCACGCCGACAGCGTGGCCGAGCTGGGCGAGCAGATCGACAACCTGCAGC GGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACAT GGAGCAGATCATCAAGGCCAAGGCAAACCTGGAGAAAGTGTCTCGGACGCTGGAGGACCAGGCCAATGAG TACCGCGTGAAGCTAGAAGAGGCCCAACGCTCCCTCAATGATTTCACCACCCAGCGAGCCAAGCTGCAGA CCGAGAATGGAGAGTTGGCCCGGCAGCTAGAGGAAAAGGAGGCGCTAATCTCGCAGCTGACCCGGGGGAA GCTCTCTTATACCCAGCAAATGGAGGACCTCAAAAGGCAGCTGGAGGAGGAGGGCAAGGCGAAGAACGCC CTGGCCCATGCACTGCAGTCGGCCCGGCATGACTGCGACCTGCTGCGGGAGCAGTACGAGGAGGAGACAG AGGCCAAGGCCGAGCTGCAGCGCGTCCTGTCCAAGGCCAACTCGGAGGTGGCCCAGTGGAGGACCAAGTA TGAGACGGACGCCATTCAGCGGACTGAGGAGCTCGAAGAGGCCAAAAAGAAGCTGGCCCAGCGGCTGCAG GATGCCGAGGAGGCCGTGGAGGCTGTTAATGCCAAGTGCTCCTCACTGGAGAAGACCAAGCACCGGCTAC AGAATGAGATAGAGGACTTGATGGTGGACGTAGAGCGCTCCAATGCTGCTGCTGCAGCCCTGGACAAGAA GCAGAGAAACTTTGACAAGATCCTGGCCGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCTGAGCTGGAG TCCTCACAGAAGGAGGCTCGCTCCCTCAGCACAGAGCTCTTCAAGCTCAAGAACGCCTACGAGGAGTCCC TGGAGCACCTAGAGACCTTCAAGCGGGAGAACAAGAACCTTCAGGAGGAAATCTCGGACCTTACTGAGCA GCTAGGAGAAGGAGGAAAGAATGTGCATGAGCTGGAGAAGGTCCGCAAACAGCTGGAGGTGGAGAAGCTG GAGCTGCAGTCAGCCCTGGAGGAGGCAGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGGGCCC AGCTAGAGTTCAACCAGATCAAGGCAGAGATCGAGCGGAAGCTGGCAGAGAAGGACGAGGAGATGGAACA GGCCAAGCGCAACCACCAGCGGGTGGTGGACTCGCTGCAGACCTCCCTGGATGCAGAGACACGCAGCCGC AACGAGGTCCTGAGGGTGAAGAAGAAGATGGAAGGAGACCTCAATGAGATGGAGATCCAGCTCAGCCACG CCAACCGCATGGCTGCCGAGGCCCAGAAGCAAGTCAAGAGCCTCCAGAGCTTGCTGAAGGACACCCAGAT CCAGCTGGACGATGCGGTCCGTGCCAACGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAAC AACCTGCTGCAGGCTGAGCTGGAGGAGCTGCGTGCCGTGGTGGAGCAGACAGAGCGGTCCCGGAAGCTGG CGGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCATTCCCAGAACACCAGCCTCATCAA CCAGAAGAAGAAGATGGAGTCGGATCTGACCCAGCTCCAGTCGGAAGTGGAGGAGGCAGTGCAGGAGTGC AGAAACGCCGAGGAGAAGGCCAAGAAGGCCATCACGGATGCCGCCATGATGGCAGAGGAGCTGAAGAAGG AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATTAAGGACCTGCAGCA CCGGCTGGACGAGGCCGAGCAGATCGCCCTCAAGGGAGGCAAGAAGCAGCTGCAGAAGCTGGAAGCGCGG GTGCGGGAGCTGGAGGGTGAGCTGGAGGCCGAGCAGAAGCGCAACGCAGAGTCGGTGAAGGGCATGAGGA AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAAAAGAACCTGCTGCGGCTACA GGACCTGGTGGACAAGCTGCAACTGAAGGTCAAGGCCTACAAGCGCCAGGCCGAGGAGGCGGAGGAGCAA GCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAGCATGAGCTGGATGAGGCAGAGGAGCGGGCGGACA TCGCTGAGTCCCAGGTCAACAAGCTTCGAGCCAAGAGCCGTGACATTGGTGCCAAGCAAAAAATGCACGA TGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213673 representing NM_002471
Red=Cloning site Green=Tags(s) MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEFVKAKILSREGGKVIAETENGKTVT VKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVA AYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNA NANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKA ERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGV YKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQ VYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGK SNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADT GDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGV LEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAG LLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKP LLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQL IKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNL TEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDL ERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEEL EEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEAT AAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANE YRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNA LAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQ DAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELE SSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKL ELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSR NEVLRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRN NLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQSEVEEAVQEC RNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEAR VRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQ ANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002471 |
| ORF Size | 5817 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002471.4 |
| RefSeq Size | 5941 bp |
| RefSeq ORF | 5820 bp |
| Locus ID | 4624 |
| UniProt ID | P13533 |
| Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Tight junction, Viral myocarditis |
| MW | 224.2 kDa |
| Gene Summary | Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213673L1 | Lenti ORF clone of Human myosin, heavy chain 6, cardiac muscle, alpha (MYH6), Myc-DDK-tagged |
CNY 21,952.00 |
|
| RG213673 | MYH6 (tGFP-tagged) - Human myosin, heavy chain 6, cardiac muscle, alpha (MYH6) |
CNY 20,900.00 |
|
| SC309209 | MYH6 (untagged)-Human myosin, heavy chain 6, cardiac muscle, alpha (MYH6) |
CNY 19,560.00 |
