Collagen IV (COL4A1) (NM_001845) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BSVD; BSVD1; COL4A1s; PADMAL; RATOR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213796 ORF sequence, codon optimized. Due to the complexity of NM_001845, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCGAGACTGTCTGTATGGTTGCTCCTGCTGCCCGCTGCCCTGCTGCTTCACGAGGAGCATTCCC GGGCTGCCGCGAAAGGGGGGTGTGCAGGCAGCGGCTGTGGTAAATGTGATTGTCACGGCGTCAAGGGCCA GAAGGGAGAGCGGGGTCTGCCGGGTCTGCAAGGTGTGATCGGATTTCCCGGAATGCAGGGTCCAGAGGGC CCACAGGGCCCACCCGGCCAGAAAGGCGACACCGGAGAGCCAGGCCTGCCTGGCACCAAAGGAACACGGG GACCACCTGGGGCTAGCGGATACCCTGGAAACCCCGGGCTGCCGGGGATTCCCGGCCAGGACGGGCCCCC CGGTCCACCCGGAATTCCAGGGTGTAACGGTACCAAGGGAGAACGCGGCCCACTGGGTCCCCCGGGCTTG CCTGGGTTCGCAGGCAACCCGGGACCCCCTGGGCTGCCGGGGATGAAGGGAGATCCCGGCGAAATCCTTG GTCATGTTCCCGGGATGCTGCTCAAGGGCGAAAGGGGATTCCCAGGCATCCCGGGGACGCCCGGTCCGCC TGGACTGCCAGGTCTGCAGGGACCTGTGGGCCCACCCGGGTTTACTGGGCCCCCAGGGCCACCTGGACCA CCCGGACCCCCAGGGGAGAAGGGACAGATGGGGCTGTCTTTTCAGGGTCCGAAAGGGGACAAAGGGGACC AAGGCGTGAGTGGCCCTCCTGGAGTGCCCGGCCAGGCCCAGGTACAGGAGAAGGGCGATTTTGCTACTAA AGGCGAAAAGGGACAAAAGGGTGAACCTGGCTTTCAAGGGATGCCGGGGGTTGGAGAGAAGGGGGAGCCA GGAAAACCAGGCCCGAGAGGCAAGCCTGGGAAGGATGGAGACAAAGGAGAGAAAGGAAGCCCCGGCTTTC CCGGTGAACCAGGGTACCCAGGACTGATTGGGCGCCAGGGACCACAGGGTGAGAAAGGAGAAGCAGGCCC TCCTGGACCTCCAGGAATCGTCATTGGAACAGGACCATTGGGGGAGAAGGGCGAGAGAGGATACCCCGGC ACCCCAGGGCCGCGGGGCGAACCAGGGCCTAAGGGGTTTCCAGGGCTGCCAGGTCAGCCAGGTCCTCCAG GTCTGCCGGTGCCAGGCCAGGCCGGAGCTCCTGGCTTCCCTGGTGAGAGAGGCGAAAAAGGCGACAGAGG TTTTCCAGGCACTTCTTTGCCTGGCCCGTCCGGCAGGGATGGCCTGCCGGGACCACCGGGCAGTCCCGGG CCCCCCGGTCAGCCTGGATATACAAATGGCATCGTGGAGTGTCAACCAGGACCCCCCGGAGATCAAGGCC CACCCGGAATCCCAGGGCAGCCTGGATTCATTGGCGAGATTGGAGAGAAGGGGCAGAAAGGGGAATCATG CCTGATATGTGATATTGATGGTTACAGAGGGCCACCCGGGCCCCAGGGCCCCCCCGGGGAAATCGGCTTT CCAGGTCAGCCAGGTGCAAAGGGGGACAGGGGACTGCCAGGAAGGGACGGGGTAGCCGGCGTGCCAGGCC CCCAGGGCACCCCTGGACTCATTGGTCAGCCCGGTGCCAAGGGTGAACCTGGGGAATTCTATTTTGATCT GCGCCTCAAAGGCGACAAAGGGGACCCGGGCTTCCCAGGCCAGCCCGGAATGCCTGGCAGAGCCGGGAGC CCAGGGCGAGATGGACACCCAGGTCTGCCAGGACCCAAGGGCAGCCCAGGAAGCGTTGGCCTGAAAGGGG AACGCGGTCCTCCCGGGGGCGTGGGATTCCCGGGAAGCCGGGGTGATACAGGACCTCCTGGCCCTCCTGG ATATGGTCCAGCTGGCCCTATCGGGGATAAGGGGCAGGCAGGGTTCCCTGGAGGGCCTGGGAGCCCCGGG CTTCCTGGACCAAAAGGCGAGCCTGGAAAAATTGTCCCTCTTCCCGGGCCACCTGGAGCGGAGGGTCTGC CAGGCTCACCCGGCTTTCCGGGACCCCAGGGGGACCGGGGATTCCCTGGTACACCCGGCCGCCCCGGCCT GCCCGGAGAAAAAGGGGCAGTAGGACAGCCTGGGATCGGATTCCCGGGACCGCCTGGACCCAAGGGAGTC GATGGGCTGCCGGGCGATATGGGGCCACCAGGCACCCCCGGACGGCCCGGATTTAACGGCCTGCCCGGAA ACCCCGGTGTACAAGGTCAGAAAGGCGAGCCAGGCGTGGGACTGCCTGGACTGAAAGGGCTGCCCGGCCT GCCTGGAATTCCCGGTACCCCCGGCGAGAAGGGAAGTATCGGTGTGCCCGGGGTGCCAGGAGAACATGGC GCGATTGGGCCCCCGGGACTGCAGGGGATTCGCGGCGAGCCCGGCCCCCCAGGGCTGCCCGGGAGCGTCG GCAGTCCTGGGGTGCCCGGCATCGGGCCACCCGGGGCCAGAGGACCGCCTGGGGGACAGGGCCCCCCGGG CCTTAGCGGCCCCCCAGGAATCAAAGGCGAAAAGGGATTCCCCGGCTTTCCAGGACTTGATATGCCTGGG CCTAAAGGGGACAAAGGGGCACAGGGGCTTCCAGGAATCACTGGGCAATCTGGACTCCCCGGGCTCCCCG GTCAGCAGGGCGCACCCGGCATTCCGGGATTCCCTGGCAGCAAGGGAGAAATGGGCGTTATGGGGACCCC CGGTCAGCCAGGCTCACCCGGACCCGTGGGTGCACCCGGACTTCCAGGAGAAAAGGGAGATCATGGCTTT CCAGGCTCCAGCGGCCCACGGGGCGACCCAGGACTGAAAGGAGATAAGGGAGATGTGGGACTGCCTGGCA AGCCTGGCTCCATGGACAAGGTAGACATGGGGAGCATGAAGGGCCAGAAGGGCGATCAGGGAGAGAAGGG ACAAATTGGTCCTATCGGTGAAAAGGGCAGTAGGGGGGATCCTGGTACACCCGGAGTGCCAGGAAAAGAT GGGCAGGCAGGACAGCCTGGACAGCCCGGGCCCAAGGGAGATCCCGGAATCTCTGGGACCCCTGGAGCTC CCGGACTCCCCGGACCCAAGGGATCAGTAGGGGGCATGGGCTTGCCTGGAACCCCCGGCGAGAAAGGGGT GCCGGGGATCCCGGGCCCTCAAGGATCTCCAGGGCTTCCTGGCGATAAAGGGGCAAAAGGGGAAAAAGGC CAAGCCGGCCCACCTGGAATTGGAATCCCTGGCCTTCGAGGAGAGAAGGGCGACCAAGGAATAGCAGGCT TTCCCGGGTCCCCGGGCGAAAAAGGGGAGAAAGGAAGTATCGGAATTCCAGGCATGCCTGGTTCTCCAGG TCTGAAGGGATCACCCGGCTCAGTCGGTTATCCTGGCAGTCCCGGGTTGCCAGGCGAGAAGGGCGATAAA GGACTTCCAGGCCTGGATGGAATACCCGGTGTCAAGGGAGAGGCAGGCCTCCCTGGTACTCCCGGGCCCA CCGGCCCCGCCGGACAAAAAGGCGAACCCGGTTCCGATGGAATTCCAGGGTCTGCGGGGGAGAAGGGGGA ACCCGGGCTCCCCGGGCGCGGATTCCCGGGGTTTCCCGGGGCCAAAGGAGACAAGGGATCCAAAGGGGAG GTAGGGTTCCCTGGTCTGGCCGGCTCCCCGGGCATTCCAGGAAGTAAAGGGGAGCAAGGTTTTATGGGGC CCCCCGGGCCTCAGGGTCAACCAGGGCTGCCTGGTAGTCCCGGCCACGCCACCGAGGGCCCCAAGGGCGA CAGGGGGCCGCAGGGTCAGCCAGGTCTGCCAGGACTGCCAGGCCCAATGGGCCCGCCTGGCCTCCCTGGC ATAGATGGGGTAAAAGGGGACAAAGGCAATCCCGGCTGGCCCGGCGCCCCTGGCGTGCCTGGACCGAAGG GAGATCCTGGTTTTCAAGGCATGCCCGGAATAGGGGGAAGCCCTGGCATCACCGGCAGCAAGGGAGACAT GGGACCGCCGGGCGTACCCGGCTTTCAGGGGCCCAAGGGCCTGCCCGGACTTCAGGGAATTAAGGGCGAT CAGGGCGACCAGGGAGTACCCGGCGCTAAGGGTCTTCCTGGGCCACCTGGTCCTCCAGGTCCCTATGATA TTATCAAAGGGGAACCAGGGTTGCCTGGGCCCGAGGGGCCTCCAGGCTTGAAAGGCCTCCAGGGACTGCC AGGACCCAAGGGACAACAGGGTGTGACCGGCCTCGTCGGCATCCCAGGACCCCCCGGCATCCCTGGATTC GACGGCGCTCCCGGCCAGAAGGGAGAAATGGGTCCTGCTGGACCTACAGGACCGCGCGGCTTCCCAGGAC CTCCGGGGCCAGATGGGTTGCCTGGTTCTATGGGGCCCCCTGGCACTCCTTCAGTTGACCACGGGTTCTT GGTGACTAGGCATAGCCAGACCATTGATGACCCACAGTGCCCCTCCGGCACCAAGATTTTGTACCACGGC TATAGCCTCCTCTATGTGCAAGGAAACGAGCGCGCGCATGGACAGGACCTGGGAACAGCCGGAAGTTGCC TGCGCAAGTTCAGCACCATGCCTTTCCTTTTTTGTAATATTAACAACGTATGCAATTTTGCCTCCCGGAA CGACTATTCCTACTGGTTGAGTACTCCGGAACCTATGCCCATGTCAATGGCCCCCATTACAGGCGAAAAC ATTCGACCCTTTATTTCAAGGTGTGCTGTCTGCGAAGCTCCAGCTATGGTGATGGCGGTGCACAGCCAAA CCATCCAAATCCCGCCCTGTCCATCAGGGTGGTCCAGCCTGTGGATTGGATATAGCTTTGTGATGCACAC GTCCGCAGGAGCTGAGGGCTCTGGCCAGGCCCTGGCAAGTCCAGGTAGCTGCTTGGAAGAGTTTCGATCA GCGCCTTTCATAGAATGTCATGGGCGGGGAACTTGCAACTACTACGCGAACGCTTATAGCTTTTGGTTGG CTACCATCGAGCGCAGCGAAATGTTTAAGAAGCCTACCCCTAGCACCCTCAAGGCTGGCGAGCTTCGGAC CCACGTGTCCCGATGCCAGGTCTGTATGCGCCGGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC213796 representing NM_001845 Red=Cloning site Green=Tags(s) MGPRLSVWLLLLPAALLLHEEHSRAAAKGGCAGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG PQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFAGNPGPPGLPGMKGDPGEILGHVPGMLLKGERGFPGIPGTPGPPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGLSFQGPKGDKGDQGVSGPPGVPGQAQVQEKGDFATKGEKGQKGEPGFQGMPGVGEKGEP GKPGPRGKPGKDGDKGEKGSPGFPGEPGYPGLIGRQGPQGEKGEAGPPGPPGIVIGTGPLGEKGERGYPG TPGPRGEPGPKGFPGLPGQPGPPGLPVPGQAGAPGFPGERGEKGDRGFPGTSLPGPSGRDGLPGPPGSPG PPGQPGYTNGIVECQPGPPGDQGPPGIPGQPGFIGEIGEKGQKGESCLICDIDGYRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGVAGVPGPQGTPGLIGQPGAKGEPGEFYFDLRLKGDKGDPGFPGQPGMPGRAGS PGRDGHPGLPGPKGSPGSVGLKGERGPPGGVGFPGSRGDTGPPGPPGYGPAGPIGDKGQAGFPGGPGSPG LPGPKGEPGKIVPLPGPPGAEGLPGSPGFPGPQGDRGFPGTPGRPGLPGEKGAVGQPGIGFPGPPGPKGV DGLPGDMGPPGTPGRPGFNGLPGNPGVQGQKGEPGVGLPGLKGLPGLPGIPGTPGEKGSIGVPGVPGEHG AIGPPGLQGIRGEPGPPGLPGSVGSPGVPGIGPPGARGPPGGQGPPGLSGPPGIKGEKGFPGFPGLDMPG PKGDKGAQGLPGITGQSGLPGLPGQQGAPGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPGLPGEKGDHGF PGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKD GQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKG QAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGSPGLPGEKGDK GLPGLDGIPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEPGLPGRGFPGFPGAKGDKGSKGE VGFPGLAGSPGIPGSKGEQGFMGPPGPQGQPGLPGSPGHATEGPKGDRGPQGQPGLPGLPGPMGPPGLPG IDGVKGDKGNPGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGPKGLPGLQGIKGD QGDQGVPGAKGLPGPPGPPGPYDIIKGEPGLPGPEGPPGLKGLQGLPGPKGQQGVTGLVGIPGPPGIPGF DGAPGQKGEMGPAGPTGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTIDDPQCPSGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGEN IRPFISRCAVCEAPAMVMAVHSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001845
ORF Size	5007 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001845.1, NM_001845.2, NM_001845.3, NM_001845.4, NM_001845.5, NP_001836.1
RefSeq Size	6540 bp
RefSeq ORF	5010 bp
Locus ID	1282
UniProt ID	P02462
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	160.6 kDa
Gene Summary	This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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