Tenascin N (TNN) (NM_022093) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TN-W; TNW
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214108 ORF sequence, codon optimized. Due to the complexity of NM_022093, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTCCAGGAGATGTTTAGGTTTCCAATGGGGCTGCTCCTCGGGAGCGTGCTGCTGGTGGCATCCG CCCCCGCCACACTCGAACCTCCCGGCTGCTCTAACAAAGAGCAACAGGTCACTGTGAGCCACACATACAA GATCGATGTCCCAAAAAGTGCACTGGTACAGGTGGATGCCGATCCCCAGCCACTGTCAGACGACGGAGCC AGCCTGCTCGCACTGGGGGAAGCCCGCGAGGAGCAGAACATCATTTTCAGGCACAACATCCGACTGCAGA CCCCGCAGAAGGATTGCGAACTCGCCGGTTCCGTGCAGGATTTGCTTGCTCGCGTCAAGAAGTTGGAGGA GGAGATGGTTGAAATGAAAGAACAATGCTCTGCTCAGCGGTGTTGTCAGGGCGTCACAGATTTGTCACGA CATTGTAGCGGACATGGTACCTTTTCCCTTGAAACCTGCTCCTGCCACTGCGAAGAAGGGCGGGAAGGTC CAGCCTGCGAGAGGTTGGCCTGCCCCGGGGCCTGCAGCGGTCATGGCCGATGTGTGGATGGACGGTGTTT GTGCCACGAGCCATACGTAGGGGCCGACTGCGGGTACCCCGCCTGCCCGGAGAACTGCAGCGGACACGGG GAGTGTGTCCGGGGGGTTTGCCAGTGCCACGAGGATTTTATGTCCGAAGACTGTTCCGAAAAGAGGTGCC CTGGAGACTGCTCCGGTCACGGCTTTTGTGATACAGGCGAATGTTATTGCGAGGAAGGTTTTACAGGCCT GGACTGCGCACAAGTCGTGACCCCCCAGGGGCTGCAACTTCTCAAGAATACAGAGGACTCCTTGCTGGTT TCCTGGGAGCCCTCTTCCCAAGTGGATCACTACCTCCTCTCTTACTATCCTCTTGGCAAGGAACTCTCCG GGAAGCAAATTCAGGTGCCCAAGGAACAGCACTCTTACGAAATCCTGGGACTTCTGCCCGGGACAAAATA TATTGTCACACTCCGAAATGTAAAGAATGAAGTGTCTTCCTCCCCTCAGCACCTTCTGGCCACAACAGAC CTGGCCGTCCTGGGCACTGCTTGGGTGACTGACGAGACAGAAAACAGTCTGGACGTGGAATGGGAAAATC CATCCACTGAGGTGGACTATTACAAGCTGAGATATGGACCCATGACTGGTCAAGAGGTCGCGGAGGTGAC AGTCCCTAAAAGTTCAGATCCCAAGTCCAGATACGATATAACCGGGCTGCACCCAGGCACAGAATACAAG ATAACTGTGGTGCCAATGCGGGGAGAGCTGGAGGGTAAGCCCATTCTGCTTAATGGCCGGACTGAGATTG ATTCACCAACGAACGTGGTTACTGACAGAGTGACTGAGGACACTGCGACCGTGTCATGGGATCCCGTTCA GGCCGTCATTGACAAATACGTCGTTCGGTACACCTCCGCTGATGGCGACACCAAAGAAATGGCGGTCCAC AAGGATGAGAGCAGCACGGTTCTGACTGGGTTGAAGCCAGGTGAAGCTTATAAGGTGTATGTCTGGGCCG AGAGAGGTAACCAGGGGTCTAAAAAAGCTGACACAAACGCTCTCACAGAAATTGACAGCCCGGCCAATCT CGTCACTGACCGAGTGACCGAGAATACCGCAACCATTTCTTGGGATCCTGTGCAGGCCACAATAGACAAG TACGTAGTTCGGTACACTTCAGCAGACGACCAGGAAACCAGAGAAGTGCTTGTTGGGAAGGAGCAGTCTA GCACAGTCCTTACAGGGCTTCGCCCCGGAGTCGAATATACCGTGCACGTCTGGGCGCAGAAGGGGGATAG GGAATCTAAGAAAGCCGACACCAACGCACCCACTGACATAGATTCCCCGAAAAACCTGGTTACAGACAGG GTAACAGAGAATATGGCCACCGTGTCATGGGACCCCGTCCAGGCAGCAATCGACAAGTACGTGGTGCGCT ACACCAGCGCCGGCGGCGAGACAAGGGAAGTGCCAGTTGGCAAGGAGCAATCTTCCACAGTGCTCACAGG TCTGCGCCCCGGAATGGAATACATGGTACACGTTTGGGCCCAAAAAGGCGATCAAGAGTCAAAAAAAGCG GATACAAAGGCCCAGACGGATATTGACTCACCGCAGAACCTTGTGACTGATAGGGTAACTGAGAACATGG CTACAGTGAGCTGGGACCCTGTCCGGGCCACAATTGACAGGTACGTGGTCCGCTACACTTCCGCGAAGGA TGGCGAAACCCGGGAAGTTCCAGTGGGCAAGGAACAAAGCAGCACAGTACTGACCGGCCTGCGGCCCGGA GTGGAGTATACCGTACACGTCTGGGCCCAAAAAGGTGCTCAAGAGAGCAAAAAGGCCGATACCAAGGCGC AAACCGACATCGACAGCCCACAAAACCTGGTGACCGACTGGGTCACAGAGAATACTGCCACCGTTAGCTG GGACCCTGTTCAGGCGACCATCGACAGATACGTTGTTCACTATACCTCAGCTAACGGCGAAACAAGGGAG GTGCCTGTCGGGAAAGAACAGAGCAGTACTGTGCTTACCGGACTGAGGCCCGGTATGGAATATACCGTCC ATGTCTGGGCGCAGAAAGGAAACCAGGAGAGCAAGAAAGCCGACACCAAGGCACAGACAGAGATCGATGG ACCTAAGAATCTGGTGACGGATTGGGTCACTGAGAACATGGCCACAGTGTCATGGGATCCCGTGCAAGCT ACTATTGACAAGTATATGGTGCGCTACACTAGCGCAGATGGGGAAACACGAGAGGTACCCGTGGGAAAGG AGCATTCCTCAACAGTACTCACTGGCCTGCGACCAGGGATGGAATATATGGTGCACGTGTGGGCACAGAA AGGTGCCCAGGAGTCTAAGAAGGCCGATACAAAGGCACAGACAGAACTCGATCCACCACGGAATCTTAGA CCCAGCGCAGTTACCCAGTCCGGAGGCATTTTGACTTGGACTCCTCCCTCTGCCCAGATCCACGGATACA TTCTCACCTATCAGTTCCCAGATGGGACTGTAAAGGAAATGCAGCTTGGGAGGGAAGATCAGCGCTTTGC TTTGCAGGGGCTGGAGCAAGGGGCTACTTACCCCGTGTCTCTTGTGGCTTTCAAGGGCGGTAGGAGAAGC AGGAATGTGAGCACTACCCTGTCAACTGTAGGCGCCCGCTTTCCTCACCCAAGCGATTGCAGCCAGGTGC AACAGAATTCTAATGCTGCCTCCGGGCTGTATACAATATATCTCCACGGTGATGCGAGCCGCCCTCTCCA GGTGTACTGCGACATGGAAACCGATGGTGGCGGTTGGATCGTTTTCCAGAGACGAAACACTGGTCAGTTG GATTTCTTCAAGCGCTGGCGCTCATATGTGGAGGGATTTGGGGATCCAATGAAGGAGTTCTGGCTGGGCC TGGATAAACTGCATAATCTTACCACTGGCACCCCTGCCAGATACGAGGTCCGCGTCGACCTGCAGACTGC CAATGAAAGCGCCTATGCCATTTACGACTTCTTTCAGGTGGCCAGCAGTAAGGAGAGGTACAAACTCACC GTTGGGAAATACAGAGGGACCGCAGGCGATGCCCTTACCTACCACAACGGCTGGAAGTTCACCACTTTCG ATCGAGACAATGACATTGCCCTTTCCAACTGCGCCCTCACCCACCATGGCGGGTGGTGGTATAAGAACTG CCATTTGGCCAACCCAAATGGAAGATACGGAGAGACAAAGCACTCCGAAGGGGTCAATTGGGAACCTTGG AAAGGTCATGAATTCTCTATCCCTTACGTTGAACTCAAGATTCGGCCACATGGATACTCAAGAGAGCCAG TGCTGGGTCGGAAGAAGCGGACCCTGCGGGGAAGACTGAGAACATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214108 representing NM_022093 Red=Cloning site Green=Tags(s) MSLQEMFRFPMGLLLGSVLLVASAPATLEPPGCSNKEQQVTVSHTYKIDVPKSALVQVDADPQPLSDDGA SLLALGEAREEQNIIFRHNIRLQTPQKDCELAGSVQDLLARVKKLEEEMVEMKEQCSAQRCCQGVTDLSR HCSGHGTFSLETCSCHCEEGREGPACERLACPGACSGHGRCVDGRCLCHEPYVGADCGYPACPENCSGHG ECVRGVCQCHEDFMSEDCSEKRCPGDCSGHGFCDTGECYCEEGFTGLDCAQVVTPQGLQLLKNTEDSLLV SWEPSSQVDHYLLSYYPLGKELSGKQIQVPKEQHSYEILGLLPGTKYIVTLRNVKNEVSSSPQHLLATTD LAVLGTAWVTDETENSLDVEWENPSTEVDYYKLRYGPMTGQEVAEVTVPKSSDPKSRYDITGLHPGTEYK ITVVPMRGELEGKPILLNGRTEIDSPTNVVTDRVTEDTATVSWDPVQAVIDKYVVRYTSADGDTKEMAVH KDESSTVLTGLKPGEAYKVYVWAERGNQGSKKADTNALTEIDSPANLVTDRVTENTATISWDPVQATIDK YVVRYTSADDQETREVLVGKEQSSTVLTGLRPGVEYTVHVWAQKGDRESKKADTNAPTDIDSPKNLVTDR VTENMATVSWDPVQAAIDKYVVRYTSAGGETREVPVGKEQSSTVLTGLRPGMEYMVHVWAQKGDQESKKA DTKAQTDIDSPQNLVTDRVTENMATVSWDPVRATIDRYVVRYTSAKDGETREVPVGKEQSSTVLTGLRPG VEYTVHVWAQKGAQESKKADTKAQTDIDSPQNLVTDWVTENTATVSWDPVQATIDRYVVHYTSANGETRE VPVGKEQSSTVLTGLRPGMEYTVHVWAQKGNQESKKADTKAQTEIDGPKNLVTDWVTENMATVSWDPVQA TIDKYMVRYTSADGETREVPVGKEHSSTVLTGLRPGMEYMVHVWAQKGAQESKKADTKAQTELDPPRNLR PSAVTQSGGILTWTPPSAQIHGYILTYQFPDGTVKEMQLGREDQRFALQGLEQGATYPVSLVAFKGGRRS RNVSTTLSTVGARFPHPSDCSQVQQNSNAASGLYTIYLHGDASRPLQVYCDMETDGGGWIVFQRRNTGQL DFFKRWRSYVEGFGDPMKEFWLGLDKLHNLTTGTPARYEVRVDLQTANESAYAIYDFFQVASSKERYKLT VGKYRGTAGDALTYHNGWKFTTFDRDNDIALSNCALTHHGGWWYKNCHLANPNGRYGETKHSEGVNWEPW KGHEFSIPYVELKIRPHGYSREPVLGRKKRTLRGRLRTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022093
ORF Size	3897 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022093.1, NP_071376.1
RefSeq Size	5008 bp
RefSeq ORF	3900 bp
Locus ID	63923
UniProt ID	Q9UQP3
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	144 kDa
Gene Summary	Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1 (By similarity) (PubMed:17909022). Involved in neurite outgrowth and cell migration in hippocampal explants (By similarity). During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling (By similarity). In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327). Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells (PubMed:17909022).[UniProtKB/Swiss-Prot Function]

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