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CDC40 (NM_015891) Human Tagged ORF Clone
CAT＃: RC214712
CDC40 (Myc-DDK-tagged)-Human cell division cycle 40 homolog (S. cerevisiae) (CDC40)
ORF Plasmid: tGFP
"NM_015891" in other vectors (6)
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CNY 4,312.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EHB3; PCH15; PRP17; PRPF17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214712 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTGCGATTGCAGCTCTGGCCGCTTCCTATGGTTCGGGTTCAGGGTCCGAATCGGACTCGGACA GTGAGAGCAGTCGGTGTCCGCTGCCAGCCGCCGACTCCCTCATGCACTTGACTAAATCGCCTTCATCAAA GCCGTCTCTAGCAGTGGCAGTGGACTCGGCTCCGGAGGTGGCAGTTAAGGAAGATTTGGAGACTGGAGTT CACCTTGACCCTGCCGTCAAAGAAGTTCAGTATAATCCTACCTATGAGACCATGTTTGCTCCTGAGTTTG GACCAGAAAATCCCTTTAGGACACAGCAAATGGCTGCCCCTAGAAATATGCTTTCTGGATATGCCGAACC AGCTCATATCAATGATTTCATGTTTGAGCAGCAAAGGAGAACTTTTGCAACATATGGTTATGCATTAGAC CCTTCATTAGATAATCATCAAGTGTCTGCTAAATATATTGGTTCTGTAGAAGAAGCTGAAAAAAATCAAG GTTTAACTGTATTTGAAACTGGTCAGAAGAAAACAGAAAAGAGGAAAAAGTTTAAAGAAAATGATGCATC CAATATTGATGGTTTTTTGGGACCATGGGCAAAATATGTGGATGAAAAAGATGTAGCCAAACCTTCAGAA GAAGAGCAAAAAGAATTGGATGAAATCACAGCAAAGAGGCAGAAAAAAGGAAAACAGGAAGAAGAGAAAC CTGGGGAGGAGAAGACAATCTTACATGTTAAAGAAATGTATGACTATCAAGGCAGGTCCTATCTTCACAT ACCTCAGGATGTTGGTGTTAATCTACGGTCAACTATGCCACCTGAGAAGTGTTATCTTCCCAAAAAACAA ATTCATGTGTGGTCTGGACACACAAAGGGCGTCAGTGCAGTCAGATTGTTTCCTCTCTCTGGCCATTTAT TGCTGTCTTGTTCCATGGACTGTAAAATTAAGCTATGGGAGGTTTATGGAGAACGGCGCTGTCTGAGAAC ATTTATTGGTCACAGTAAGGCTGTTAGGGATATCTGCTTCAATACTGCAGGAACACAGTTCCTCAGTGCA GCCTATGACAGGTATCTTAAGCTCTGGGACACTGAGACAGGACAGTGTATATCAAGATTTACAAACCGAA AAGTACCTTATTGTGTCAAATTCAATCCTGATGAAGATAAGCAAAATCTCTTTGTGGCTGGGATGTCTGA TAAGAAGATTGTGCAATGGGACATTCGAAGTGGAGAAATTGTGCAGGAATATGATCGGCATTTGGGAGCT GTCAACACCATTGTTTTTGTGGATGAGAATAGGAGATTTGTGAGCACATCTGATGATAAAAGCCTAAGAG TTTGGGAATGGGATATCCCTGTGGATTTCAAGTACATAGCAGAACCCAGTATGCACTCAATGCCTGCAGT GACTTTGTCTCCAAATGGAAAATGGCTAGCATGCCAATCAATGGACAACCAAATCTTAATTTTTGGAGCA CAGAACAGATTTAGATTAAATAAGAAAAAAATTTTTAAGGGCCATATGGTAGCAGGCTATGCTTGTCAGG TGGACTTTTCACCAGACATGAGTTATGTGATTTCAGGAGATGGAAATGGAAAATTAAACATTTGGGACTG GAAGACCACAAAACTCTACAGTCGATTTAAAGCTCATGATAAAGTGTGTATAGGTGCAGTGTGGCATCCT CATGAAACTTCTAAGGTCATAACATGTGGTTGGGATGGTCTCATTAAATTGTGGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214712 protein sequence
Red=Cloning site Green=Tags(s) MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGV HLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALD PSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSE EEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQ IHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSA AYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGA VNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGA QNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHP HETSKVITCGWDGLIKLWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015891 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015891.3 |
| RefSeq Size | 3866 bp |
| RefSeq ORF | 1740 bp |
| Locus ID | 51362 |
| UniProt ID | O60508 |
| Domains | WD40 |
| Protein Pathways | Spliceosome |
| MW | 65.5 kDa |
| Gene Summary | Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214712L1 | Lenti ORF clone of Human cell division cycle 40 homolog (S. cerevisiae) (CDC40), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214712L2 | Lenti ORF clone of Human cell division cycle 40 homolog (S. cerevisiae) (CDC40), mGFP tagged |
CNY 6,460.00 |
|
| RC214712L3 | Lenti ORF clone of Human cell division cycle 40 homolog (S. cerevisiae) (CDC40), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214712L4 | Lenti ORF clone of Human cell division cycle 40 homolog (S. cerevisiae) (CDC40), mGFP tagged |
CNY 6,460.00 |
|
| RG214712 | CDC40 (tGFP-tagged) - Human cell division cycle 40 homolog (S. cerevisiae) (CDC40) |
CNY 5,040.00 |
|
| SC314085 | CDC40 (untagged)-Human cell division cycle 40 homolog (S. cerevisiae) (CDC40) |
CNY 7,220.00 |
