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RBM15 (NM_022768) Human Tagged ORF Clone
CAT＃: RC214777
- TrueORF®
RBM15 (Myc-DDK-tagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1
ORF Plasmid: tGFP
"NM_022768" in other vectors (7)
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CNY 7,152.00
CNY 9,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OTT; OTT1; SPEN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214777 representing NM_022768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACTGCGGGGCGGGACCCTGTGCCGCGGCGGAGTCCAAGATGGCGGCGTGCGGTTCCGCTGTGTG AAACGAGCGCGGGGCGGCGGGTTACTCAGCTCCGCGGAGACGACCTCCGACGACCCGCAACAATGAAGGG AAAAGAGCGCTCGCCAGTGAAGGCCAAACGCTCCCGTGGTGGTGAGGACTCGACTTCCCGCGGTGAGCGG AGCAAGAAGTTAGGGGGCTCTGGTGGCAGCAATGGGAGCAGCAGCGGAAAGACCGATAGCGGCGGTGGGT CGCGGCGGAGTCTCCACCTGGACAAGTCCAGCAGTCGAGGTGGCAGCCGCGAGTATGATACCGGTGGGGG CAGCTCCAGTAGCCGCTTGCATAGTTATAGCTCCCCGAGCACCAAAAATTCTTCGGGCGGGGGCGAGTCG CGCAGCAGCTCCCGGGGTGGAGGCGGGGAGTCACGTTCCTCTGGGGCCGCCTCCTCAGCTCCCGGCGGCG GGGACGGCGCGGAATACAAGACTCTGAAGATAAGCGAGTTGGGGTCCCAGCTTAGTGACGAAGCGGTGGA GGACGGCCTGTTTCATGAGTTCAAACGCTTCGGTGATGTAAGTGTGAAAATCAGTCATCTGTCGGGTTCT GGCAGCGGGGATGAGCGGGTAGCCTTTGTGAACTTCCGGCGGCCAGAGGACGCGCGGGCGGCCAAGCATG CCAGAGGCCGCCTGGTGCTCTATGACCGGCCTCTGAAGATAGAAGCTGTGTATGTGAGCCGGCGCCGCAG CCGCTCCCCTTTAGACAAAGATACTTATCCTCCATCAGCCAGTGTGGTCGGGGCCTCTGTAGGTGGTCAC CGGCACCCCCCTGGAGGTGGTGGAGGCCAGAGATCACTTTCCCCTGGTGGCGCTGCTTTGGGATACAGAG ACTACCGGCTGCAGCAGTTGGCTCTTGGCCGCCTGCCCCCTCCACCTCCGCCACCATTGCCTCGAGACCT GGAGAGAGAAAGAGACTACCCGTTCTATGAGAGAGTGCGCCCTGCATACAGTCTTGAGCCAAGGGTGGGA GCTGGAGCAGGTGCTGCTCCTTTCAGAGAAGTGGATGAGATTTCACCCGAGGATGATCAGCGAGCTAACC GGACGCTCTTCTTGGGCAACCTAGACATCACTGTAACGGAGAGTGATTTAAGAAGGGCGTTTGATCGCTT TGGAGTCATCACAGAAGTAGATATCAAGAGGCCTTCTCGCGGCCAGACTAGTACTTACGGCTTTCTCAAA TTTGAGAACTTAGATATGTCTCACCGGGCCAAATTAGCAATGTCTGGCAAAATTATAATTCGGAATCCTA TCAAAATTGGTTATGGTAAAGCTACACCCACCACCCGCCTCTGGGTGGGAGGCCTGGGACCTTGGGTTCC TCTTGCTGCCCTGGCACGAGAATTTGATCGATTTGGCACCATACGCACCATAGACTACCGAAAAGGTGAT AGTTGGGCATATATCCAGTATGAAAGCCTGGATGCAGCGCATGCTGCCTGGACCCATATGCGGGGCTTCC CACTTGGTGGCCCAGATCGACGCCTTAGAGTAGACTTTGCCGACACCGAACATCGTTACCAGCAGCAGTA TCTGCAGCCTCTGCCCTTGACTCATTATGAGCTGGTGACAGATGCTTTTGGACATCGGGCACCAGACCCT TTGAGGGGTGCTCGGGATAGGACACCACCCTTACTATACAGAGATCGTGATAGGGACCTTTATCCTGACT CTGATTGGGTGCCACCCCCACCCCCAGTCCGAGAACGCAGCACTCGGACTGCAGCTACTTCTGTGCCTGC TTACGAGCCACTGGATAGCCTAGATCGCAGGCGGGATGGTTGGTCCTTGGACCGGGACAGAGGTGATCGA GATCTGCCCAGCAGCAGAGACCAGCCTAGGAAGCGAAGGCTGCCTGAGGAGAGTGGAGGACGTCATCTGG ATAGGTCTCCTGAGAGTGACCGCCCACGAAAACGTCACTGCGCTCCTTCTCCTGACCGCAGTCCAGAATT GAGCAGTAGCCGGGATCGTTACAACAGCGACAATGATCGATCTTCCCGTCTTCTCTTGGAAAGGCCCTCT CCAATCAGAGACAGACGAGGTAGTTTGGAGAAGAGCCAGGGTGACAAGCGAGACCGTAAAAACTCTGCAT CAGCTGAACGAGATAGGAAGCACCGGACAACTGCTCCCACTGAGGGAAAAAGCCCTCTGAAAAAAGAAGA CCGCTCTGATGGGAGTGCACCTAGCACCAGCACTGCTTCCTCCAAGCTGAAGTCCCCGTCCCAGAAACAG GATGGGGGGACAGCCCCTGTGGCATCAGCCTCTCCCAAACTCTGTTTGGCCTGGCAGGGCATGCTTCTAC TGAAGAACAGCAACTTTCCTTCCAACATGCATCTGTTGCAGGGTGACCTCCAAGTGGCTAGTAGTCTTCT TGTGGAGGGTTCAACTGGAGGCAAAGTGGCCCAGCTCAAGATCACTCAGCGTCTCCGTTTGGACCAGCCC AAGTTGGATGAAGTAACTCGACGCATCAAAGTAGCAGGGCCCAATGGTTATGCCATTCTTTTGGCTGTGC CTGGAAGTTCTGACAGCCGGTCCTCCTCTTCCTCAGCTGCATCAGACACTGCCACTTCTACTCAGAGGCC ACTTAGGAACCTTGTGTCCTATTTAAAGCAAAAGCAGGCAGCCGGGGTGATCAGCCTCCCTGTGGGGGGC AACAAAGACAAGGAAAACACCGGGGTCCTTCATGCCTTCCCACCTTGTGAGTTCTCCCAGCAGTTCCTGG ATTCCCCTGCCAAGGCACTGGCCAAATCTGAAGAAGATTACCTGGTCATGATCATTGTCCGTGGGTTTGG TTTTCAGATAGGAGTTAGGTATGAGAACAAGAAGAGAGAAAACTTGGCGCTGACCCTGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214777 representing NM_022768
Red=Cloning site Green=Tags(s) MRTAGRDPVPRRSPRWRRAVPLCETSAGRRVTQLRGDDLRRPATMKGKERSPVKAKRSRGGEDSTSRGER SKKLGGSGGSNGSSSGKTDSGGGSRRSLHLDKSSSRGGSREYDTGGGSSSSRLHSYSSPSTKNSSGGGES RSSSRGGGGESRSSGAASSAPGGGDGAEYKTLKISELGSQLSDEAVEDGLFHEFKRFGDVSVKISHLSGS GSGDERVAFVNFRRPEDARAAKHARGRLVLYDRPLKIEAVYVSRRRSRSPLDKDTYPPSASVVGASVGGH RHPPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVG AGAGAAPFREVDEISPEDDQRANRTLFLGNLDITVTESDLRRAFDRFGVITEVDIKRPSRGQTSTYGFLK FENLDMSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGD SWAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDAFGHRAPDP LRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDR DLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPS PIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQ DGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQP KLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGG NKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022768 |
| ORF Size | 2931 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022768.4, NP_073605.4 |
| RefSeq Size | 3450 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 64783 |
| UniProt ID | Q96T37 |
| Protein Families | Transcription Factors |
| MW | 107 kDa |
| Gene Summary | Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214777L1 | Lenti-ORF clone of RBM15 (Myc-DDK-tagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 11,690.00 |
|
| RC214777L2 | Lenti-ORF clone of RBM15 (mGFP-tagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 9,552.00 |
|
| RC214777L3 | Lenti-ORF clone of RBM15 (Myc-DDK-tagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 11,690.00 |
|
| RC214777L4 | Lenti-ORF clone of RBM15 (mGFP-tagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 11,690.00 |
|
| RG214777 | RBM15 (tGFP-tagged) - Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 10,740.00 |
|
| SC122934 | RBM15 (untagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 6,784.00 |
|
| SC318429 | RBM15 (untagged)-Human RNA binding motif protein 15 (RBM15), transcript variant 1 |
CNY 18,810.00 |
