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DPYD (NM_000110) Human Tagged ORF Clone
CAT＃: RC216374
DPYD (Myc-DDK-tagged)-Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1
ORF Plasmid: tGFP
"NM_000110" in other vectors (4)
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CNY 7,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DHP; DHPDHASE; DPD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216374 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCTGTGCTCAGTAAGGACTCGGCGGACATCGAGAGTATCCTGGCTTTAAATCCTCGAACACAAA CTCATGCAACTCTGTGTTCCACTTCGGCCAAGAAATTAGACAAGAAACATTGGAAAAGAAATCCTGATAA GAACTGCTTTAATTGTGAGAAGCTGGAGAATAATTTTGATGACATCAAGCACACGACTCTTGGTGAGCGA GGAGCTCTCCGAGAAGCAATGAGATGCCTGAAATGTGCAGATGCCCCGTGTCAGAAGAGCTGTCCAACTA ATCTTGATATTAAATCATTCATCACAAGTATTGCAAACAAGAACTATTATGGAGCTGCTAAGATGATATT TTCTGACAACCCACTTGGTCTGACTTGTGGAATGGTATGTCCAACCTCTGATCTTTGTGTAGGTGGATGC AATTTATATGCCACTGAAGAGGGACCCATTAATATTGGTGGATTGCAGCAATTTGCTACTGAGGTATTCA AAGCAATGAGTATCCCACAGATCAGAAATCCTTCGCTGCCTCCCCCAGAAAAAATGTCTGAAGCCTATTC TGCAAAGATTGCTCTTTTTGGTGCTGGGCCTGCAAGTATAAGTTGTGCTTCCTTTTTGGCTCGATTGGGG TACTCTGACATCACTATATTTGAAAAACAAGAATATGTTGGTGGTTTAAGTACTTCTGAAATTCCTCAGT TCCGGCTGCCGTATGATGTAGTGAATTTTGAGATTGAGCTAATGAAGGACCTTGGTGTAAAGATAATTTG CGGTAAAAGCCTTTCAGTGAATGAAATGACTCTTAGCACTTTGAAAGAAAAAGGCTACAAAGCTGCTTTC ATTGGAATAGGTTTGCCAGAACCCAATAAAGATGCCATCTTCCAAGGCCTGACGCAGGACCAGGGGTTTT ATACATCCAAAGACTTTTTGCCACTTGTAGCCAAAGGCAGTAAAGCAGGAATGTGCGCCTGTCACTCTCC ATTGCCATCGATACGGGGAGTCGTGATTGTACTTGGAGCTGGAGACACTGCCTTTGACTGTGCAACATCT GCTCTACGTTGTGGAGCTCGCCGTGTGTTCATCGTCTTCAGAAAAGGCTTTGTTAATATAAGAGCTGTCC CTGAGGAGATGGAACTTGCTAAGGAAGAAAAGTGTGAATTTCTGCCATTCCTGTCCCCACGGAAGGTTAT AGTAAAAGGTGGGAGAATTGTTGCTATGCAGTTTGTTCGGACAGAGCAAGATGAAACTGGAAAATGGAAT GAAGATGAAGATCAGATGGTCCATCTGAAAGCCGATGTGGTCATCAGTGCCTTTGGTTCAGTTCTGAGTG ATCCTAAAGTAAAAGAAGCCTTGAGCCCTATAAAATTTAACAGATGGGGTCTCCCAGAAGTAGATCCAGA AACTATGCAAACTAGTGAAGCATGGGTATTTGCAGGTGGTGATGTCGTTGGTTTGGCTAACACTACAGTG GAATCGGTGAATGATGGAAAGCAAGCTTCTTGGTACATTCACAAATACGTACAGTCACAATATGGAGCTT CCGTTTCTGCCAAGCCTGAACTACCCCTCTTTTACACTCCTATTGATCTGGTGGACATTAGTGTAGAAAT GGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGCGCAACTCCAGCCACCAGCACATCAATGATT CGAAGAGCTTTTGAAGCTGGATGGGGTTTTGCCCTCACCAAAACTTTCTCTCTTGATAAGGACATTGTGA CAAATGTTTCCCCCAGAATCATCCGGGGAACCACCTCTGGCCCCATGTATGGCCCTGGACAAAGCTCCTT TCTGAATATTGAGCTCATCAGTGAGAAAACGGCTGCATATTGGTGTCAAAGTGTCACTGAACTAAAGGCT GACTTTCCAGACAACATTGTGATTGCTAGCATTATGTGCAGTTACAATAAAAATGACTGGACGGAACTTG CCAAGAAGTCTGAGGATTCTGGAGCAGATGCCCTGGAGTTAAATTTATCATGTCCACATGGCATGGGAGA AAGAGGAATGGGCCTGGCCTGTGGGCAGGATCCAGAGCTGGTGCGGAACATCTGCCGCTGGGTTAGGCAA GCTGTTCAGATTCCTTTTTTTGCCAAGCTGACCCCAAATGTCACTGATATTGTGAGCATCGCAAGAGCTG CAAAGGAAGGTGGTGCCAATGGCGTTACAGCCACCAACACTGTCTCAGGTCTGATGGGATTAAAATCTGA TGGCACACCTTGGCCAGCAGTGGGGATTGCAAAGCGAACTACATATGGAGGAGTGTCTGGGACAGCAATC AGACCTATTGCTTTGAGAGCTGTGACCTCCATTGCTCGTGCTCTGCCTGGATTTCCCATTTTGGCTACTG GTGGAATTGACTCTGCTGAAAGTGGTCTTCAGTTTCTCCATAGTGGTGCTTCCGTCCTCCAGGTATGCAG TGCCATTCAGAATCAGGATTTCACTGTGATCGAAGACTACTGCACTGGCCTCAAAGCCCTGCTTTATCTG AAAAGCATTGAAGAACTACAAGACTGGGATGGACAGAGTCCAGCTACTGTGAGTCACCAGAAAGGGAAAC CAGTTCCACGTATAGCTGAACTCATGGACAAGAAACTGCCAAGTTTTGGACCTTATCTGGAACAGCGCAA GAAAATCATAGCAGAAAACAAGATTAGACTGAAAGAACAAAATGTAGCTTTTTCACCACTTAAGAGAAAC TGTTTTATCCCCAAAAGGCCTATTCCTACCATCAAGGATGTAATAGGAAAAGCACTGCAGTACCTTGGAA CATTTGGTGAATTGAGCAACGTAGAGCAAGTTGTGGCTATGATTGATGAAGAAATGTGTATCAACTGTGG TAAATGCTACATGACCTGTAATGATTCTGGCTACCAGGCTATACAGTTTGATCCAGAAACCCACCTGCCC ACCATAACCGACACTTGTACAGGCTGTACTCTGTGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAA TGGTTTCCAGGACAACACCTTATGAACCAAAGAGAGGCGTACCCTTATCTGTGAATCCGGTGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216374 protein sequence
Red=Cloning site Green=Tags(s) MAPVLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGER GALREAMRCLKCADAPCQKSCPTNLDIKSFITSIANKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGC NLYATEEGPINIGGLQQFATEVFKAMSIPQIRNPSLPPPEKMSEAYSAKIALFGAGPASISCASFLARLG YSDITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSVNEMTLSTLKEKGYKAAF IGIGLPEPNKDAIFQGLTQDQGFYTSKDFLPLVAKGSKAGMCACHSPLPSIRGVVIVLGAGDTAFDCATS ALRCGARRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLSPRKVIVKGGRIVAMQFVRTEQDETGKWN EDEDQMVHLKADVVISAFGSVLSDPKVKEALSPIKFNRWGLPEVDPETMQTSEAWVFAGGDVVGLANTTV ESVNDGKQASWYIHKYVQSQYGASVSAKPELPLFYTPIDLVDISVEMAGLKFVNPFGLASATPATSTSMI RRAFEAGWGFALTKTFSLDKDIVTNVSPRIIRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKA DFPDNIVIASIMCSYNKNDWTELAKKSEDSGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQ AVQIPFFAKLTPNVTDIVSIARAAKEGGANGVTATNTVSGLMGLKSDGTPWPAVGIAKRTTYGGVSGTAI RPIALRAVTSIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAIQNQDFTVIEDYCTGLKALLYL KSIEELQDWDGQSPATVSHQKGKPVPRIAELMDKKLPSFGPYLEQRKKIIAENKIRLKEQNVAFSPLKRN CFIPKRPIPTIKDVIGKALQYLGTFGELSNVEQVVAMIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLP TITDTCTGCTLCLSVCPIVDCIKMVSRTTPYEPKRGVPLSVNPVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000110 |
| ORF Size | 3075 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000110.4 |
| RefSeq Size | 4451 bp |
| RefSeq ORF | 3078 bp |
| Locus ID | 1806 |
| UniProt ID | Q12882 |
| Domains | DHOdehase, fer4 |
| Protein Families | Druggable Genome |
| Protein Pathways | beta-Alanine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Pantothenate and CoA biosynthesis, Pyrimidine metabolism |
| MW | 111.4 kDa |
| Gene Summary | The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216374L3 | Lenti ORF clone of Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC216374L4 | Lenti ORF clone of Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1, mGFP tagged |
CNY 9,536.00 |
|
| RG216374 | DPYD (tGFP-tagged) - Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1 |
CNY 8,736.00 |
|
| SC316297 | DPYD (untagged)-Human dihydropyrimidine dehydrogenase (DPYD), transcript variant 1 |
CNY 7,144.00 |
