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Symplekin (SYMPK) (NM_004819) Human Tagged ORF Clone
CAT＃: RC216742
SYMPK (Myc-DDK-tagged)-Human symplekin (SYMPK)
ORF Plasmid: tGFP
"NM_004819" in other vectors (7)
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CNY 8,872.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pta1; SPK; SYM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216742 representing NM_004819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGGCAGTGGAGACAGCGTCACCCGTCGGAGCGTGGCATCACAGTTTTTCACTCAAGAGGAGG GGCCGGGCATCGATGGCATGACCACCTCAGAGAGGGTGGTGGATCTTCTGAACCAGGCGGCGCTGATCAC CAATGACTCAAAGATCACAGTGCTCAAACAGGTCCAGGAGCTGATCATCAACAAAGACCCCACACTACTG GACAACTTCCTGGATGAGATCATCGCATTCCAAGCAGACAAGTCAATCGAAGTGCGAAAATTTGTCATCG GCTTCATCGAGGAGGCATGCAAGCGAGACATTGAGTTGCTGCTGAAACTCATTGCAAACCTCAACATGCT CTTGAGGGACGAGAATGTGAACGTGGTGAAGAAGGCTATCCTCACCATGACCCAGCTCTACAAGGTGGCC CTGCAGTGGATGGTAAAGTCACGGGTCATTAGCGAGCTACAGGAGGCCTGCTGGGACATGGTATCTGCCA TGGCGGGGGACATCATCCTGCTATTGGACTCTGACAATGACGGCATCCGCACCCACGCCATCAAGTTTGT GGAGGGCCTCATTGTCACCCTGTCACCCCGCATGGCTGACTCAGAGATACCCCGACGCCAGGAGCATGAT ATCAGCCTGGACCGCATCCCTCGTGACCACCCCTACATCCAGTACAACGTGCTATGGGAAGAGGGCAAGG CAGCCTTGGAGCAGCTGCTTAAGTTCATGGTGCACCCTGCCATCTCCTCCATCAACCTGACCACAGCGCT GGGCTCCCTTGCCAATATCGCCCGCCAGAGACCCATGTTCATGTCTGAGGTGATCCAGGCCTATGAAACT CTGCATGCCAACCTGCCCCCGACGCTGGCCAAATCGCAGGTGAGCAGTGTGCGTAAGAATCTGAAGCTGC ACCTGTTGAGTGTGCTGAAGCACCCGGCTTCCTTGGAGTTCCAGGCCCAGATCACCACCCTGCTGGTGGA CCTGGGCACACCTCAGGCCGAGATCGCCCGCAACATGCCGAGCAGCAAGGACACCCGCAAGCGGCCCCGC GATGACTCGGACTCCACACTCAAGAAGATGAAGCTGGAGCCCAACCTGGGGGAGGACGATGAGGACAAAG ACTTGGAGCCAGGCCCGTCGGGGACCTCGAAGGCCTCAGCGCAGATCTCCGGCCAGTCAGACACGGACAT CACAGCTGAGTTCCTGCAGCCTCTGCTGACGCCTGATAATGTGGCTAATCTGGTCCTCATCAGCATGGTG TACCTACCCGAGGCCATGCCAGCCTCCTTCCAGGCCATCTACACCCCCGTGGAGTCAGCAGGCACGGAAG CCCAGATCAAGCACCTGGCTCGGCTCATGGCCACACAGATGACAGCTGCCGGACTGGGACCAGGTGTAGA GCAGACCAAACAGTGCAAGGAGGAGCCCAAGGAGGAGAAGGTGGTGAAGACAGAGAGCGTCCTGATCAAG CGGCGCCTGTCAGCCCAGGGCCAAGCCATCTCGGTGGTGGGTTCCCTGAGCTCCATGTCCCCCCTGGAGG AAGAGGCACCGCAGGCCAAGAGGAGGCCAGAGCCCATTATCCCTGTCACTCAGCCCCGGCTGGCAGGCGC TGGTGGGCGCAAGAAAATTTTCCGTCTCAGCGACGTGCTGAAGCCCCTTACCGATGCCCAGGTGGAAGCC ATGAAGCTGGGCGCTGTGAAGCGGATCCTGCGGGCTGAGAAGGCTGTGGCCTGCAGCGGGGCAGCCCAGG TCCGCATAAAGATCCTGGCCAGCCTGGTGACACAGTTCAACTCGGGCCTGAAGGCGGAGGTCCTGTCCTT CATCCTGGAGGATGTGCGGGCCCGCCTGGACCTGGCCTTCGCCTGGCTCTACCAGGAGTACAACGCCTAC CTGGCCGCAGGTGCCTCGGGCTCCCTGGACAAGTATGAGGACTGCCTCATCCGCCTGTTGTCTGGCCTGC AGGAGAAACCAGACCAGAAGGATGGGATCTTCACCAAGGTTGTGCTGGAGGCGCCACTCATCACAGAGAG TGCCCTGGAGGTGGTCCGCAAGTACTGCGAGGATGAGAGTCGCACCTATCTGGGCATGTCCACACTTCGA GACCTGATCTTCAAGCGCCCGTCCCGCCAGTTCCAGTACCTGCATGTCCTCCTCGACCTCAGCTCCCATG AGAAGGACAAGGTGCGCTCCCAGGCCCTGCTGTTCATCAAACGCATGTATGAGAAGGAGCAGCTGCGGGA GTATGTGGAGAAATTTGCCCTCAACTACCTGCAGCTCCTGGTGCACCCCAACCCACCGTCTGTGCTGTTT GGAGCTGACAAGGACACAGAGGTGGCAGCACCCTGGACGGAGGAGACAGTGAAGCAGTGTCTGTACCTCT ACCTGGCCCTCCTGCCTCAGAACCACAAGCTGATCCACGAACTGGCGGCCGTGTACACTGAAGCCATCGC CGACATCAAGCGGACGGTGCTGAGGGTCATTGAGCAGCCGATCCGAGGAATGGGCATGAACTCCCCGGAG CTGCTCCTGCTGGTGGAAAATTGTCCCAAGGGAGCAGAGACACTGGTCACGAGATGTCTGCACAGCCTCA CAGACAAAGTCCCACCCTCCCCAGAGCTGGTGAAGCGGGTCCGGGATCTCTACCACAAGCGACTGCCAGA CGTCCGCTTCCTCATCCCGGTGCTCAATGGGCTGGAGAAGAAAGAGGTGATCCAGGCCCTGCCTAAACTC ATCAAACTCAACCCCATCGTGGTGAAGGAAGTCTTCAACCGCCTGCTGGGCACCCAGCATGGTGAGGGAA ACTCAGCCTTGTCCCCGCTGAACCCTGGAGAGCTCCTGATCGCATTACACAACATTGACTCCGTGAAGTG CGACATGAAATCCATCATCAAAGCCACCAACCTGTGCTTTGCGGAGCGGAACGTGTACACGTCAGAGGTG CTGGCCGTGGTGATGCAGCAGCTGATGGAGCAGAGCCCCCTGCCCATGCTGCTCATGAGGACCGTCATCC AGTCCCTGACCATGTACCCCCGCCTGGGGGGCTTCGTCATGAACATCCTGTCCCGCCTCATCATGAAGCA GGTGTGGAAGTACCCCAAGGTGTGGGAGGGCTTCATCAAGTGCTGCCAGCGCACAAAGCCCCAGAGCTTC CAGGTCATCCTGCAGCTGCCGCCCCAGCAGCTGGGAGCCGTCTTTGACAAGTGCCCAGAGCTCCGGGAGC CCCTGCTGGCCCATGTCCGCTCCTTCACCCCCCACCAGCAAGCTCACATCCCTAACTCCATCATGACCAT CTTGGAGGCCAGCGGCAAGCAGGAGCCAGAGGCCAAGGAGGCGCCTGCGGGGCCCTTGGAGGAGGATGAT CTGGAGCCCCTGACCTTGGCCCCGGCCCCAGCACCCCGGCCCCCTCAGGACCTCATCGGCCTGCGACTGG CCCAGGAGAAGGCCTTAAAGCGGCAGCTGGAGGAGGAACAGAAGCTGAAGCCGGGAGGAGTGGGAGCCCC CTCCTCTTCCTCCCCCTCTCCCTCTCCGTCGGCCCGGCCAGGCCCGCCCCCGTCTGAGGAAGCCATGGAT TTCCGGGAGGAGGGGCCTGAGTGCGAGACCCCGGGCATCTTCATCAGCATGGATGACGACTCGGGGCTGA CCGAGGCCGCGCTGTTGGACTCTAGTCTCGAGGGCCCCCTACCCAAGGAGACGGCAGCGGGCGGGCTGAC CTTGAAGGAGGAGCGGAGCCCCCAGACCCTCGCACCTGTTGGAGAAGATGCTATGAAGACTCCCAGCCCG GCTGCCGAGGACGCCAGGGAACCCGAGGCCAAGGGGAACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216742 representing NM_004819
Red=Cloning site Green=Tags(s) MASGSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLL DNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVA LQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHD ISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYET LHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPR DDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMV YLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIK RRLSAQGQAISVVGSLSSMSPLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEA MKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFNSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAY LAAGASGSLDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVVRKYCEDESRTYLGMSTLR DLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLF GADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPE LLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKL IKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAERNVYTSEV LAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSF QVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMTILEASGKQEPEAKEAPAGPLEEDD LEPLTLAPAPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGVGAPSSSSPSPSPSARPGPPPSEEAMD FREEGPECETPGIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLTLKEERSPQTLAPVGEDAMKTPSP AAEDAREPEAKGNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004819 |
| ORF Size | 3822 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004819.3 |
| RefSeq Size | 4188 bp |
| RefSeq ORF | 3825 bp |
| Locus ID | 8189 |
| UniProt ID | Q92797 |
| Protein Pathways | Tight junction |
| MW | 141 kDa |
| Gene Summary | This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
JTE-607, a multiple cytokine production inhibitor, targets CPSF3 and inhibits pre-mRNA processing
,Kakegawa, J;Sakane, N;Suzuki, K;Yoshida, T;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31399191
[Symplekin]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216742L1 | Lenti ORF clone of Human symplekin (SYMPK), Myc-DDK-tagged |
CNY 11,272.00 |
|
| RC216742L2 | Lenti ORF clone of Human symplekin (SYMPK), mGFP tagged |
CNY 14,630.00 |
|
| RC216742L3 | Lenti ORF clone of Human symplekin (SYMPK), Myc-DDK-tagged |
CNY 14,630.00 |
|
| RC216742L4 | Lenti ORF clone of Human symplekin (SYMPK), mGFP tagged |
CNY 14,630.00 |
|
| RG216742 | SYMPK (tGFP-tagged) - Human symplekin (SYMPK) |
CNY 13,970.00 |
|
| SC309970 | SYMPK (untagged)-Human symplekin (SYMPK) |
CNY 7,960.00 |
|
| SC318582 | SYMPK (untagged)-Human symplekin (SYMPK) |
CNY 24,700.00 |
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