ZNF493 (NM_175910) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216979 ORF sequence, codon optimized. Due to the complexity of NM_175910, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGAGTGTAACGTCCACAAGGAAGGCTACAACGAGCTGAATCAATACTTGACTACCACCCAGTCAA AGATATTTCAGTGCGATAAATATGTTAAAGTTTTTCATAAGCTTCTCAACAGTAACAGGCACAATACAAA ACATACCGGGAAGAAGCCATTCAAATGTAAAAAATGTGGAAAGAGCTTTTGTATGCTTCTCCACCTGTGC CAACACAAGCGAATCCACATTCGCGAAAACTCCTACAGATGCGAGGAGTGTGGCAAGGCCTTTATCTGGT TTTCAACATTGACCCGGCACCGCAGGGTGCACACCGGTGAAAAGTCTTACAAGTACGAGTGCGGCAAATC ATTCAATCAGGACAGTAATTTGACTACTCACAAACGGATACATACAGGCCAGAAACCCTATAAGTGTGAG GAATGCGGCACATCTTTTTACCAGTTCAGTTATCTCACGCGGCATAAGTTGATCCACACGCGAGAAAAAC CATATAAATGTGAGCAGTATGGAAAGACCTTCAACCAGTCCTCCACTCTGACCGGCCACAAGATCATCCA CAATGGGGAAAAACCCTACAAATGCGAAGAATGTGGAAAGGCCTTTTCAATTTTTTCTACACCCACTAAG CATAAGATTATTCACACCGAGGAGAAGTCTCATCGCTGCGAGGAGTATTGCAAGGCCTATAAAGAGAGCA GTCACCTTACCACTCACAAGCGCATTCACACCGGAGAAAAGCCTTACAAATGTGAAGAGTGCGGGAAGGC TTTTTCCATTTTTTCTACCCTGACCAAACATAAGATTATTCACACAGAGGAGAAGAGTCACAGGTGTGAG GAATGTGGAAAAGCATATAAGGAGTCTTCTCACCTGACTACCCATAAGAGAATACATACAGGAGAAAAAC CATACAAATGCGAGGAATGTGGGAAAACTTTTAGCGTTTTTTCTATTTTGACCAAGCATAAGATAATCCA TACAGAGGAAAAGCCATATAAGTGTGAGGAGTGCGGAAAAGCTTTCAAAAGGTCAAGCACACTGACAAAA CACCGCATAATCCACACTGAGGAGAAGCCCTATAAATGCGAGGAGTGCGGCAAGGCCTTCAATCAGTCAT CCACCCTCTCCATCCACAAGATCATCCACACTGGGGAAAAGCCTTATAAGTGCGAAGAGTGTGGAAAGGC ATTCAAGAGATCCAGCACCCTGACTATCCACAAGATGATCCACACTGGCGAGAAACCCTATAAGTGCGAA GAGTGTGGCAAGGCGTTTAATAGATCCTCTCACCTGACTACCCACAAGCGAATCCATACTGGCCACAAAC CCTATAAATGCAAAGAGTGCGGTAAATCCTTTTCTGTCTTTAGCACGCTGACTAAGCATAAAATCATCCA CACTGACAAAAAACCCTATAAGTGCGAGGAGTGCGGAAAAGCTTTTAACCGATCTTCCATACTGTCCATC CACAAGAAAATACACACAGGAGAGAAGCCTTATAAGTGCGAGGAATGCGGCAAGGCGTTCAAGAGGAGCT CACATTTGGCCGGACACAAGCAGATCCATTCAGTGCAGAAGCCGTACAAATGCGAGGAGTGTGGCAAGGC ATTTTCTATTTTTTCAACGTTGACCAAACACAAAATCATACACACTGAAGAGAAGCCCTACAAGTGTGAG AAGTGCGGGAAAACATTCTATCGGTTCAGTAACCTGAACACCCATAAGATCATTCATACGGGGGAGAAAC CCTGCAAATGCGAGGAATGTGGCAAAGCGTTTAACCACTCATCTAACCTTATCAAGCACAAGCTCATTCA CACAGGAGACAAGCCATACAAGTGCGAAGCTTGCGGAAAAGCCTTCCGGCGGAGCTCACATCTCAGTAGA CATAAAATCATCCATATCGGTATTCATACTGAGGAGACAGTGCAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216979 representing NM_175910 Red=Cloning site Green=Tags(s) MNECNVHKEGYNELNQYLTTTQSKIFQCDKYVKVFHKLLNSNRHNTKHTGKKPFKCKKCGKSFCMLLHLC QHKRIHIRENSYRCEECGKAFIWFSTLTRHRRVHTGEKSYKYECGKSFNQDSNLTTHKRIHTGQKPYKCE ECGTSFYQFSYLTRHKLIHTREKPYKCEQYGKTFNQSSTLTGHKIIHNGEKPYKCEECGKAFSIFSTPTK HKIIHTEEKSHRCEEYCKAYKESSHLTTHKRIHTGEKPYKCEECGKAFSIFSTLTKHKIIHTEEKSHRCE ECGKAYKESSHLTTHKRIHTGEKPYKCEECGKTFSVFSILTKHKIIHTEEKPYKCEECGKAFKRSSTLTK HRIIHTEEKPYKCEECGKAFNQSSTLSIHKIIHTGEKPYKCEECGKAFKRSSTLTIHKMIHTGEKPYKCE ECGKAFNRSSHLTTHKRIHTGHKPYKCKECGKSFSVFSTLTKHKIIHTDKKPYKCEECGKAFNRSSILSI HKKIHTGEKPYKCEECGKAFKRSSHLAGHKQIHSVQKPYKCEECGKAFSIFSTLTKHKIIHTEEKPYKCE KCGKTFYRFSNLNTHKIIHTGEKPCKCEECGKAFNHSSNLIKHKLIHTGDKPYKCEACGKAFRRSSHLSR HKIIHIGIHTEETVQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175910
ORF Size	1938 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175910.4, NM_175910.5, NM_175910.6, NP_787106.3
RefSeq Size	4732 bp
RefSeq ORF	1941 bp
Locus ID	284443
UniProt ID	Q6ZR52
MW	75.3 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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