Separase (ESPL1) (NM_012291) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ESP1; SEPA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC217230 representing NM_012291 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAAGAGTTGCTGC CCGCCTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGCCGATCTGATGCTGAGAGGAG ACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAGCTGACTGCTAAGCTAGCTTGCCCTAGGCAT CTGGGGAGCCTGCTGGAGCTGGCAGAGCTGGCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTC CCCTCTACCTGGAACGAATTCTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCAC ACTCCGCCTTGCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAACGGCGAGCTG CATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGATGAAAGTACCCCTTGTGAGGT TCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCTGCCCATCAGCTATTTGATGCCAGTGGCCAT GGTCTAAATGAAGCAGATGCTGATTTCCTAGATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGG GTGAGAGAGGGAGCTCTTCTGGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGA ACACTGCCGTCGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTGCTGCAGGTTG GGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCTGTCCTGAGCAAGAGTATGGA GGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGCCAGTTCTTCCTTTCAGGCCTGGAACGAGGC ACCAAGAGGCGCTATAGACTTGATGCCATTCTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTC TGCAGCAGCTGCGGGATGATGGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTA CTTTCAGGGACTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCCTTAGAGGGCC TGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACCAGTAATTTGGCCTACAGCTT CTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCTGAGCCGCTCTGTCAGCACCTGGGTTTGGTG AAGCCAGGCACTTATCCCGAGGTGCCTCCTGAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTT TGAAGAAACTGGGTAAACAGGCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTG TAGCCCTGAACACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACCCTGGCCCTCC TGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGACAGGAACGCTTCAACATCAT CTGTGACCTCCTGGAGCTGAGCCCCGAGGAGACACCAGCCGGGGCCTGGGCACGAGCCACCCACCTGGTA GAACTGGCTCAGGTGCTCTGCTACCACGACTTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCC GGGAAGCCCTGCAGCTTCTGGACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAA AGCACAGGCCTTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTCC AGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGATGCTGCTCAGTCCAAATGCCT GGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAGGGGCAGGCCCCAGCTGTACGGTGTCTCCAG CAGACAGCAGCCTCACTGCAGATCCTAGCAGCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGG AGGTCCTCCTGCTGCTACGGATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTG CCACATCACCCAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGTGATCTGCTTC GAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTGCTGCTGTCTGTGCTTCGGGA TCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTGCGTGTCCAGGTCCTGCAGCTGGTGGCAGCT TACCTTAGCCTCCCGTCAAACAACCTCTCACACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGA CACCTGAGATAGCTCTCATAGACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGA CATTCTCTCAACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGCCTGGGTAGTG TGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAGCTGCAGATACCACGCCAGTG TGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCCCGCAATGACATTGATCTCTGTCAGTCGGAC CTGCAGCAGGTTCTGTTCTTGCTTGAGTCTTGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTG TGAAGAAGGTCCACCTGCAGAAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGA GGAGCTCTTCCTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTGTCCCATTCAC CCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGTCTGCGCTGGGTATTGGTCAC GGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGTCTGGATCTGCTGCAGGTCGTGCTGAAGGGC TGTCCTGAAGCCGCTGAGCGCCTCACCCAAGCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCT TGGTTCCAAGCCTCTTGGATGAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCA GCCATCAAACGAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTCAGCTGCTGTA CTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAAAGTGTCCCTGGCTCAGAGCC CTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGCCAAAAGTTAGCCTCTGCTCCCCTGCGCCTC AATAATACCTCTCAGAAAGGTCTGGAAGGTAGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCA GGCAAGCTGGCCCTCATGTCCCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGT ACCCCAGGCCCCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACTGCTTCCCGGG GCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAAAGACGGATGCCGTGGTTGCCCCAGGTAGTGCCCCTGG GAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAGGTGGCATCAAGACATTGTGAGGAGCGGCGT CCCCAGAGGGCCAGTGACCAGGCCAGGCCTGGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGA CTGACAACTGGAGAAAAATGAGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGG GAAGACTCCAGCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTCCGGCTCCCCT CAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCCCTGAGTGTTGCTTTCCGGGG CATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTCTGCCGCTTCCTGGCCTTGTGCCTGGGCCAC CGGGATCCTTATGCCACTGCTTTCCTTGTCACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCA CCCACCTCCACAGACAGCTCAGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCA GGGGCTGAGCCTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCTCTGATCCCCA GTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACCGTGGGCAACACCCTCCTGCT GACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAGATTCCCACTGGCCAGAACAAGCTTCATCTG CGTTCAGTCCTGAATGAGTTTGATGCCATCCAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGC GAGAATGGTGGACAGGGCGGCTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTC TGTGCTGGGCTGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAAATCATGCTCA GTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGGCTGTGCCCAACCCAGCCAGA GCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTACAGGGCCTGACAGTACCAAGCAATAGCCAC CTTGTCTTGGTCCTAGACAAGGACTTGCAGAAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGC CTGTCACCCGGCTGCCCTCCTTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCC AGTGCTGAGTCAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGGGAGGTGCCAA GACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTATGCAGGGCATGGGGCTGGTGC CCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGTCGGGCAGTGGCCCTGCTGTTTGGCTGTAGC AGTGCGGCCCTGGCTGTGCGTGGAAACCTGGAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTT GCCCCTTGTTTCTGGGTAATCTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCT GCAAGGCTGGCTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC217230 representing NM_012291 Red=Cloning site Green=Tags(s) MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRH LGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDY EAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGH GLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHS YLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERG TKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDL ADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLV KPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAG DKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLV ELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERD RRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQ QTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAA SSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAA YLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQK WQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSD LQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIA PSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKG CPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPH LEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRL NNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSD LEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERR PQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSK KKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGH RDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFR ALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHL RSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEAS RLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSH LVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSS TEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCS SAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAP RLKYLIGAAPIAYGLPVSLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012291
ORF Size	6360 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012291.5
RefSeq Size	6641 bp
RefSeq ORF	6363 bp
Locus ID	9700
UniProt ID	Q14674
Protein Families	Druggable Genome, Protease
Protein Pathways	Cell cycle, Oocyte meiosis
MW	233.2 kDa
Gene Summary	Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]

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