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CLCA1 (NM_001285) Human Tagged ORF Clone
CAT＃: RC218583
- TrueORF®
CLCA1 (Myc-DDK-tagged)-Human chloride channel accessory 1 (CLCA1)
ORF Plasmid: tGFP
"NM_001285" in other vectors (4)
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CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CACC; CaCC-1; CACC1; CLCRG1; GOB5; hCaCC-1; hCLCA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218583 representing NM_001285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCATTTAAGAGTTCTGTGTTCATCTTGATTCTTCACCTTCTAGAAGGGGCCCTGAGTAATTCAC TCATTCAGCTGAACAACAATGGCTATGAAGGCATTGTCGTTGCAATCGACCCCAATGTGCCAGAAGATGA AACACTCATTCAACAAATAAAGGACATGGTGACCCAGGCATCTCTGTATCTGTTTGAAGCTACAGGAAAG CGATTTTATTTCAAAAATGTTGCCATTTTGATTCCTGAAACATGGAAGACAAAGGCTGACTATGTGAGAC CAAAACTTGAGACCTACAAAAATGCTGATGTTCTGGTTGCTGAGTCTACTCCTCCAGGTAATGATGAACC CTACACTGAGCAGATGGGCAACTGTGGAGAGAAGGGTGAAAGGATCCACCTCACTCCTGATTTCATTGCA GGAAAAAAGTTAGCTGAATATGGACCACAAGGTAGGGCATTTGTCCATGAGTGGGCTCATCTACGATGGG GAGTATTTGACGAGTACAATAATGATGAGAAATTCTACTTATCCAATGGAAGAATACAAGCAGTAAGATG TTCAGCAGGTATTACTGGTACAAATGTAGTAAAGAAGTGTCAGGGAGGCAGCTGTTACACCAAAAGATGC ACATTCAATAAAGTAACAGGACTCTATGAAAAAGGATGTGAGTTTGTTCTCCAATCCCGCCAGACGGAGA AGGCTTCTATAATGTTTGCACAACATGTTGATTCTATAGTTGAATTCTGTACAGAACAAAACCACAACAA AGAAGCTCCAAACAAGCAAAATCAAAAATGCAATCTCCGAAGCACATGGGAAGTGATCCGTGATTCTGAG GACTTTAAGAAAACCACTCCTATGACAACACAGCCACCAAATCCCACCTTCTCATTGCTGCAGATTGGAC AAAGAATTGTGTGTTTAGTCCTTGACAAATCTGGAAGCATGGCGACTGGTAACCGCCTCAATCGACTGAA TCAAGCAGGCCAGCTTTTCCTGCTGCAGACAGTTGAGCTGGGGTCCTGGGTTGGGATGGTGACATTTGAC AGTGCTGCCCATGTACAAAATGAACTCATACAGATAAACAGTGGCAGTGACAGGGACACACTCGCCAAAA GATTACCTGCAGCAGCTTCAGGAGGGACGTCCATCTGCAGCGGGCTTCGATCGGCATTTACTGTGATTAG GAAGAAATATCCAACTGATGGATCTGAAATTGTGCTGCTGACGGATGGGGAAGACAACACTATAAGTGGG TGCTTTAACGAGGTCAAACAAAGTGGTGCCATCATCCACACAGTCGCTTTGGGGCCCTCTGCAGCTCAAG AACTAGAGGAGCTGTCCAAAATGACAGGAGGTTTACAGACATATGCTTCAGATCAAGTTCAGAACAATGG CCTCATTGATGCTTTTGGGGCCCTTTCATCAGGAAATGGAGCTGTCTCTCAGCGCTCCATCCAGCTTGAG AGTAAGGGATTAACCCTCCAGAACAGCCAGTGGATGAATGGCACAGTGATCGTGGACAGCACCGTGGGAA AGGACACTTTGTTTCTTATCACCTGGACAATGCAGCCTCCCCAAATCCTTCTCTGGGATCCCAGTGGACA GAAGCAAGGTGGCTTTGTAGTGGACAAAAACACCAAAATGGCCTACCTCCAAATCCCAGGCATTGCTAAG GTTGGCACTTGGAAATACAGTCTGCAAGCAAGCTCACAAACCTTGACCCTGACTGTCACGTCCCGTGCGT CCAATGCTACCCTGCCTCCAATTACAGTGACTTCCAAAACGAACAAGGACACCAGCAAATTCCCCAGCCC TCTGGTAGTTTATGCAAATATTCGCCAAGGAGCCTCCCCAATTCTCAGGGCCAGTGTCACAGCCCTGATT GAATCAGTGAATGGAAAAACAGTTACCTTGGAACTACTGGATAATGGAGCAGGTGCTGATGCTACTAAGG ATGACGGTGTCTACTCAAGGTATTTCACAACTTATGACACGAATGGTAGATACAGTGTAAAAGTGCGGGC TCTGGGAGGAGTTAACGCAGCCAGACGGAGAGTGATACCCCAGCAGAGTGGAGCACTGTACATACCTGGC TGGATTGAGAATGATGAAATACAATGGAATCCACCAAGACCTGAAATTAATAAGGATGATGTTCAACACA AGCAAGTGTGTTTCAGCAGAACATCCTCGGGAGGCTCATTTGTGGCTTCTGATGTCCCAAATGCTCCCAT ACCTGATCTCTTCCCACCTGGCCAAATCACCGACCTGAAGGCGGAAATTCACGGGGGCAGTCTCATTAAT CTGACTTGGACAGCTCCTGGGGATGATTATGACCATGGAACAGCTCACAAGTATATCATTCGAATAAGTA CAAGTATTCTTGATCTCAGAGACAAGTTCAATGAATCTCTTCAAGTGAATACTACTGCTCTCATCCCAAA GGAAGCCAACTCTGAGGAAGTCTTTTTGTTTAAACCAGAAAACATTACTTTTGAAAATGGCACAGATCTT TTCATTGCTATTCAGGCTGTTGATAAGGTCGATCTGAAATCAGAAATATCCAACATTGCACGAGTATCTT TGTTTATTCCTCCACAGACTCCGCCAGAGACACCTAGTCCTGATGAAACGTCTGCTCCTTGTCCTAATAT TCATATCAACAGCACCATTCCTGGCATTCACATTTTAAAAATTATGTGGAAGTGGATAGGAGAACTGCAG CTGTCAATAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218583 representing NM_001285
Red=Cloning site Green=Tags(s) MGPFKSSVFILILHLLEGALSNSLIQLNNNGYEGIVVAIDPNVPEDETLIQQIKDMVTQASLYLFEATGK RFYFKNVAILIPETWKTKADYVRPKLETYKNADVLVAESTPPGNDEPYTEQMGNCGEKGERIHLTPDFIA GKKLAEYGPQGRAFVHEWAHLRWGVFDEYNNDEKFYLSNGRIQAVRCSAGITGTNVVKKCQGGSCYTKRC TFNKVTGLYEKGCEFVLQSRQTEKASIMFAQHVDSIVEFCTEQNHNKEAPNKQNQKCNLRSTWEVIRDSE DFKKTTPMTTQPPNPTFSLLQIGQRIVCLVLDKSGSMATGNRLNRLNQAGQLFLLQTVELGSWVGMVTFD SAAHVQNELIQINSGSDRDTLAKRLPAAASGGTSICSGLRSAFTVIRKKYPTDGSEIVLLTDGEDNTISG CFNEVKQSGAIIHTVALGPSAAQELEELSKMTGGLQTYASDQVQNNGLIDAFGALSSGNGAVSQRSIQLE SKGLTLQNSQWMNGTVIVDSTVGKDTLFLITWTMQPPQILLWDPSGQKQGGFVVDKNTKMAYLQIPGIAK VGTWKYSLQASSQTLTLTVTSRASNATLPPITVTSKTNKDTSKFPSPLVVYANIRQGASPILRASVTALI ESVNGKTVTLELLDNGAGADATKDDGVYSRYFTTYDTNGRYSVKVRALGGVNAARRRVIPQQSGALYIPG WIENDEIQWNPPRPEINKDDVQHKQVCFSRTSSGGSFVASDVPNAPIPDLFPPGQITDLKAEIHGGSLIN LTWTAPGDDYDHGTAHKYIIRISTSILDLRDKFNESLQVNTTALIPKEANSEEVFLFKPENITFENGTDL FIAIQAVDKVDLKSEISNIARVSLFIPPQTPPETPSPDETSAPCPNIHINSTIPGIHILKIMWKWIGELQ LSIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001285 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001285.3, NP_001276.2 |
| RefSeq Size | 3311 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 1179 |
| UniProt ID | A8K7I4 |
| Protein Families | Druggable Genome, Ion Channels: Other |
| Protein Pathways | Olfactory transduction |
| MW | 100.26 kDa |
| Gene Summary | This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging-related kidney injury
,Lee, HJ;Donati, A;Feliers, D;Sun, Y;Ding, Y;Madesh, M;Salmon, AB;Ikeno, Y;Ross, C;O'Connor, CL;Ju, W;Bitzer, M;Chen, Y;Choudhury, GG;Singh, BB;Sharma, K;Kasinath, BS;,
Aging cell
,PubMed ID 34118180
[CLCA1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218583L3 | Lenti-ORF clone of CLCA1 (Myc-DDK-tagged)-Human chloride channel accessory 1 (CLCA1) |
CNY 9,030.00 |
|
| RC218583L4 | Lenti-ORF clone of CLCA1 (mGFP-tagged)-Human chloride channel accessory 1 (CLCA1) |
CNY 9,030.00 |
|
| RG218583 | CLCA1 (tGFP-tagged) - Human chloride channel accessory 1 (CLCA1) |
CNY 7,890.00 |
|
| SC123906 | CLCA1 (untagged)-Human chloride channel accessory 1 (CLCA1) |
CNY 10,872.00 |
