ACCN1 (ASIC2) (NM_183377) Human Tagged ORF Clone
CAT#: RC219845
ASIC2 (Myc-DDK-tagged)-Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1
ORF Plasmid: tGFP
"NM_183377" in other vectors (6)
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CNY 6,288.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ACCN; ACCN1; ASIC2a; BNaC1; BNC1; hBNaC1; MDEG |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219845 representing NM_183377
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGATTGGCGGAGCCGGGCTGCCCGCAGCCGCGCTCACCGGCCCGGGACGCTTCCGCATGGCCC GCGAGGAGCCGGCGCCCGCGGCGTTGGCGGCTGCCGGGCAGCCCGGGGGCGGCAGAGGCGGCGAGCGGGC GCTGCAGGGGCCAGGGGTCGCCCGCAGGGGGCGGCCATCGCTGAGCCGCGCTAAACTGCACGGGCTGCGG CATATGTGTGCCGGGCGCACGGCGGCTGGGGGCTCCTTCCAGCGGCGGGCGCTGTGGGTGCTGGCCTTCT GTACATCCTTCGGCTTGCTGCTGTCCTGGTCCTCGAACCGCTTGCTCTACTGGCTCAGCTTCCCGTCACA CACGCGGGTGCACCGCGAGTGGAGCCGCCAGTTACCCTTCCCCGCCGTCACTGTGTGCAACAACAACCCG CTGCGCTTCCCGCGCCTCTCCAAGGGGGACCTCTACTATGCCGGCCACTGGCTCGGGCTGCTGCTGCCCA ACCGCACCGCGCGCCCGCTTGTCAGCGAGCTGCTGCGGGGCGACGAGCCGCGCCGCCAGTGGTTCCGCAA GCTGGCGGACTTCCGCCTCTTCCTGCCTCCGCGCCACTTCGAGGGAATCAGCGCCGCCTTCATGGACCGC CTGGGCCACCAGCTGGAGGACATGCTGCTCTCCTGCAAGTACCGCGGCGAGCTCTGCGGGCCGCACAACT TCTCCTCCGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAACCTCTGCT CACCACGGTCAAGGGGGGGACAGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAGGATGAGTACCTG CCCATCTGGGGAGAGACAGAGGAAACGACATTTGAAGCAGGAGTGAAAGTTCAGATCCACAGTCAGTCTG AGCCACCTTTCATCCAAGAGCTGGGCTTTGGGGTGGCTCCAGGGTTCCAGACCTTTGTGGCCACACAGGA GCAGAGGCTCACATACCTGCCCCCACCGTGGGGTGAGTGCCGATCCTCAGAGATGGGCCTCGACTTTTTT CCTGTTTACAGCATCACCGCCTGTAGGATTGACTGTGAGACCCGCTACATTGTGGAAAACTGCAACTGCC GCATGGTTCACATGCCAGGGGATGCCCCTTTTTGTACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGC CCTAGGTCTGTTGGCGGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTAACCCGCTAC AACAAAGAGCTCTCCATGGTGAAGATCCCCAGCAAGACATCAGCCAAGTACCTTGAGAAGAAATTTAACA AATCAGAAAAATATATCTCAGAGAACATCCTTGTTCTGGATATATTTTTTGAAGCTCTCAATTATGAGAC AATTGAACAGAAGAAGGCGTATGAAGTTGCTGCCTTACTTGGTGATATTGGTGGTCAGATGGGATTGTTC ATTGGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTAT TAGACCTGCTTGGCAAAGAGGAGGACGAAGGGAGCCACGATGAGAATGTGAGTACTTGTGACACAATGCC AAACCACTCTGAAACCATCAGTCACACTGTGAACGTGCCCCTGCAGACGACCCTGGGGACCTTGGAGGAG ATTGCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219845 representing NM_183377
Red=Cloning site Green=Tags(s) MSRIGGAGLPAAALTGPGRFRMAREEPAPAALAAAGQPGGGRGGERALQGPGVARRGRPSLSRAKLHGLR HMCAGRTAAGGSFQRRALWVLAFCTSFGLLLSWSSNRLLYWLSFPSHTRVHREWSRQLPFPAVTVCNNNP LRFPRLSKGDLYYAGHWLGLLLPNRTARPLVSELLRGDEPRRQWFRKLADFRLFLPPRHFEGISAAFMDR LGHQLEDMLLSCKYRGELCGPHNFSSVFTKYGKCYMFNSGEDGKPLLTTVKGGTGNGLEIMLDIQQDEYL PIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLTYLPPPWGECRSSEMGLDFF PVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLLAEKDSNYCLCRTPCNLTRY NKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQKKAYEVAALLGDIGGQMGLF IGASILTILELFDYIYELIKEKLLDLLGKEEDEGSHDENVSTCDTMPNHSETISHTVNVPLQTTLGTLEE IAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_183377 |
ORF Size | 1689 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183377.1, NP_899233.1 |
RefSeq Size | 3470 bp |
RefSeq ORF | 1692 bp |
Locus ID | 40 |
UniProt ID | Q16515 |
Protein Families | Druggable Genome, Ion Channels: Other |
Protein Pathways | Taste transduction |
MW | 62.7 kDa |
Gene Summary | This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219845L1 | Lenti ORF clone of Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1, Myc-DDK-tagged |
CNY 8,688.00 |
|
RC219845L2 | Lenti ORF clone of Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1, mGFP tagged |
CNY 6,940.00 |
|
RC219845L3 | Lenti ORF clone of Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1, Myc-DDK-tagged |
CNY 6,940.00 |
|
RC219845L4 | Lenti ORF clone of Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1, mGFP tagged |
CNY 6,940.00 |
|
RG219845 | ASIC2 (tGFP-tagged) - Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1 |
CNY 5,510.00 |
|
SC307569 | ASIC2 (untagged)-Human amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 1 |
CNY 6,296.00 |