DGAT2L4 (AWAT2) (NM_001002254) Human Tagged ORF Clone

CAT#: RC220971

AWAT2 (Myc-DDK-tagged)-Human acyl-CoA wax alcohol acyltransferase 2 (AWAT2)

ORF Plasmid: DDK tGFP



  "NM_001002254" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-DGAT2L4 Antibody
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ARAT; DC4; DGAT2L4; MFAT; WS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220971 representing NM_001002254
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTTGCCCTCTAAGAAGGACCTCAAGACTGCCCTGGATGTCTTTGCTGTTTTCCAGTGGTCCTTCA
GTGCCTTGCTTATCACAACCACTGTGATTGCTGTCAACCTCTACCTGGTGGTGTTCACACCATACTGGCC
TGTCACTGTGCTTATTCTTACCTGGCTGGCTTTTGACTGGAAGACCCCTCAGCGAGGCGGCCGCCGGTTT
ACCTGTGTGAGGCACTGGCGCCTGTGGAAACACTACAGCGATTATTTCCCTCTCAAGCTTCTGAAGACTC
ATGACATCTGCCCCAGCCGCAACTACATCCTCGTCTGCCACCCTCATGGGCTCTTTGCCCATGGATGGTT
TGGCCACTTTGCCACAGAGGCCTCAGGCTTCTCCAAGATATTTCCTGGCATCACCCCTTACATACTCACA
CTGGGAGCCTTTTTCTGGATGCCTTTCCTCAGAGAATATGTAATGTCTACAGGGGCCTGCTCTGTGAGTC
GATCCTCCATTGACTTTCTGCTGACTCATAAAGGCACAGGCAACATGGTCATTGTGGTGATTGGTGGACT
GGCTGAGTGCAGATACAGCCTGCCAGGTTCTTCTACCCTGGTGTTGAAGAACCGGTCTGGCTTTGTGCGC
ATGGCCCTTCAGCATGGGGTGCCTCTAATACCTGCCTATGCCTTTGGGGAGACGGACCTCTATGATCAGC
ACATTTTCACTCCTGGTGGCTTTGTCAACCGCTTCCAGAAGTGGTTCCAGAGCATGGTTCACATCTACCC
TTGTGCTTTCTATGGACGTGGCTTCACCAAGAACTCCTGGGGCCTTCTGCCCTATAGTCGGCCTGTAACC
ACCATCGTCGGGGAGCCTCTACCAATGCCCAAGATTGAGAATCCAAGCCAGGAGATCGTGGCTAAATATC
ACACACTCTATATTGATGCCCTACGTAAACTGTTTGACCAGCATAAGACCAAGTTTGGTATCTCAGAGAC
CCAGGAGCTGGAGATAATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220971 representing NM_001002254
Red=Cloning site Green=Tags(s)

MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRF
TCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILT
LGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVR
MALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVT
TIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001002254
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001002254.1, NP_001002254.1
RefSeq Size 2124 bp
RefSeq ORF 1002 bp
Locus ID 158835
UniProt ID Q6E213
Protein Families Transmembrane
Protein Pathways Glycerolipid metabolism, Metabolic pathways, Retinol metabolism
MW 37.9 kDa
Gene Summary This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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