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MIER1 (NM_020948) Human Tagged ORF Clone
CAT＃: RC223165
- TrueORF®
MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 1
ORF Plasmid: tGFP
"NM_020948" in other vectors (4)
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CNY 3,936.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ER1; MI-ER1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223165 representing NM_020948
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTATGTTTAATTGGTTTACAGACTGTCTGTGGACTCTTTTCCTGTCAAATTACCAGCCATCTGTTG AATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATCCATCAGCTGACATGCTGGT TCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGGAGAAACAAACTTCAGCTCT GAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTA GTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGA TGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGT CAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCTTCTCAAGATGCCCAGGAAA TAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAGAAGAATCTGAAGAAGATGA AGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCATGTTTCAAGCAGAAATTCCA GTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTG AGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGG TGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTTATATGAATTGGTTAAATGC AATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCAGCTAGAGAGGAATTATCTG TTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATGGAAAGGATTTTCATTTGAT TCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTATTACATGTGGAAAAAATCT GAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAA CGGATTACATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCC AACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAAT GGAGTGTCATCTAATGGACCAGGTGAAATATTAAACAAAGAGGAAGTAAAAGTTGAAGGGTTACACATTA ATGGACCAACAGGTGGAAATAAGAAACCACTTCATGCAGATATGGATACTAATGGTTATGAAACAGATAA CCTTACCACTGACCCAAAACTTGCCCATATGACTGCAAGAAATGAAAATGATTTTGATGAAAAAAGTGAG AGACCTGCCAAAAGGCGAAGGGTAAACAGCAATGGAAAAGAAAGTCCAGGTTCTTCTGAATTTTTCCAAG AAGCAGTCTCACATGGGAAATTTGAAGAACTTGAAAACACAGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223165 representing NM_020948
Red=Cloning site Green=Tags(s) MFMFNWFTDCLWTLFLSNYQPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSS EIEDLAREGDMPIHELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSG QEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIP VGICRYKENEKVYENDDQLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKC NFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKS ERYDFFAQQTRFGKKKYNLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQN GVSSNGPGEILNKEEVKVEGLHINGPTGGNKKPLHADMDTNGYETDNLTTDPKLAHMTARNENDFDEKSE RPAKRRRVNSNGKESPGSSEFFQEAVSHGKFEELENTDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020948 |
| ORF Size | 1587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020948.4 |
| RefSeq Size | 5643 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 57708 |
| UniProt ID | Q8N108 |
| Domains | ELM2, myb_DNA-binding |
| MW | 60.2 kDa |
| Gene Summary | This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223165L3 | Lenti-ORF clone of MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 1 |
CNY 6,460.00 |
|
| RC223165L4 | Lenti-ORF clone of MIER1 (mGFP-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 1 |
CNY 6,460.00 |
|
| RG223165 | MIER1 (tGFP-tagged) - Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 1 |
CNY 5,040.00 |
|
| SC100475 | MIER1 (untagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 1 |
CNY 12,648.00 |
