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SMAP1 (NM_021940) Human Tagged ORF Clone
CAT＃: RC224031
- TrueORF®
SMAP1 (Myc-DDK-tagged)-Human small ArfGAP 1 (SMAP1), transcript variant 2
ORF Plasmid: tGFP
"NM_021940" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SMAP-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224031 representing NM_021940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGCGCTCCTGTCGGGAGAAGGCTCAGAAGCTGAACGAGCAGCACCAGCTCATCCTATCCAAGC TTCTGAGGGAGGAGGACAACAAGTACTGCGCCGACTGCGAGGCCAAAGGTCCTCGATGGGCTTCCTGGAA TATTGGTGTGTTTATTTGCATCAGATGTGCTGGAATTCATAGAAATCTTGGGGTTCATATATCCAGGGTC AAATCAGTCAACCTAGACCAATGGACAGCAGAACAGATACAGTGCATGCAAGATATGGGAAATACTAAAG CAAGACTACTCTATGAAGCCAATCTTCCAGAGAACTTTCGAAGACCACAGACAGATCAAGCAGTGGAATT TTTCATCAGAGATAAATATGAAAAGAAGAAATACTACGATAAAAATGCCATAGCTATTACAAATAAAGAA AAGGAAAAAAAAAAGGAAGAGAAAAAGAGAGAAAAGGAGCCAGAAAAGCCGGCAAAACCACTTACAGCTG AAAAGCTGCAGAAGAAAGATCAGCAACTGGAGCCTAAAAAAAGTACCAGCCCTAAAAAAGCTGCGGAGCC CACTGTGGATCTTTTAGGACTTGATGGCCCTGCTGTGGCACCAGTGACCAACGGGAACACAACGGTGCCA CCCCTGAACGATGATCTGGACATCTTTGGACCGATGATTTCTAATCCCTTACCTGCAACTGTCATGCCCC CAGCTCAGGGGACACCCTCTGCACCAGCAGCTGCAACCCTGTCTACAGTAACATCTGGGGATCTAGATTT ATTCACTGAGCAAACTACAAAATCAGAAGAAGTGGCAAAGAAACAACTTTCCAAAGACTCCATCTTATCT CTGTATGGCACAGGAACCATTCAACAGCAAAGTACTCCTGGTGTATTTATGGGACCCACAAATATACCAT TTACCTCACAAGCACCAGCTGCATTTCAGGGCTTTCCATCGATGGGCGTGCCTGTGCCTGCAGCTCCTGG CCTTATAGGAAATGTGATGGGACAGAGTCCAAGCATGATGGTGGGCATGCCCATGCCCAATGGGTTTATG GGAAATGCACAAACTGGTGTGATGCCACTTCCTCAGAACGTTGTTGGCCCCCAAGGAGGAATGGTGGGAC AAATGGGTGCACCCCAGAGTAAGTTTGGCCTGCCGCAAGCTCAGCAGCCCCAGTGGAGCCTCTCACAGAT GAATCAGCAGATGGCTGGCATGAGTATCAGTAGTGCAACCCCTACTGCAGGTTTTGGCCAGCCCTCCAGC ACAACAGCAGGATGGTCTGGAAGCTCATCAGGTCAGACTCTCAGCACACAACTGTGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224031 representing NM_021940
Red=Cloning site Green=Tags(s) MATRSCREKAQKLNEQHQLILSKLLREEDNKYCADCEAKGPRWASWNIGVFICIRCAGIHRNLGVHISRV KSVNLDQWTAEQIQCMQDMGNTKARLLYEANLPENFRRPQTDQAVEFFIRDKYEKKKYYDKNAIAITNKE KEKKKEEKKREKEPEKPAKPLTAEKLQKKDQQLEPKKSTSPKKAAEPTVDLLGLDGPAVAPVTNGNTTVP PLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSGDLDLFTEQTTKSEEVAKKQLSKDSILS LYGTGTIQQQSTPGVFMGPTNIPFTSQAPAAFQGFPSMGVPVPAAPGLIGNVMGQSPSMMVGMPMPNGFM GNAQTGVMPLPQNVVGPQGGMVGQMGAPQSKFGLPQAQQPQWSLSQMNQQMAGMSISSATPTAGFGQPSS TTAGWSGSSSGQTLSTQLWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021940 |
| ORF Size | 1320 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021940.5 |
| RefSeq Size | 3268 bp |
| RefSeq ORF | 1323 bp |
| Locus ID | 60682 |
| UniProt ID | Q8IYB5 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| MW | 48.1 kDa |
| Gene Summary | The protein encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224031L3 | Lenti-ORF clone of SMAP1 (Myc-DDK-tagged)-Human small ArfGAP 1 (SMAP1), transcript variant 2 |
CNY 5,890.00 |
|
| RC224031L4 | Lenti-ORF clone of SMAP1 (mGFP-tagged)-Human small ArfGAP 1 (SMAP1), transcript variant 2 |
CNY 5,890.00 |
|
| RG224031 | SMAP1 (tGFP-tagged) - Human small ArfGAP 1 (SMAP1), transcript variant 2 |
CNY 7,088.00 |
|
| SC310388 | SMAP1 (untagged)-Human small ArfGAP 1 (SMAP1), transcript variant 2 |
CNY 7,030.00 |
