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NT5C1A (NM_032526) Human Tagged ORF Clone
CAT＃: RC224617
- TrueORF®
NT5C1A (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic IA (NT5C1A)
ORF Plasmid: tGFP
"NM_032526" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CN-I; CN-IA; CN1; CN1A; CNI |
| Vector | pCMV6-Entry2 |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224617 representing NM_032526
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTGGGCAGCCCCGGGAGCCCCAGGAGCCCCGCGAGCCCGGGCCAGGAGCGGAGACCGCTGCGG CCCCGGTCTGGGAGGAAGCCAAGATTTTCTACGACAACCTCGCGCCCAAGAAGAAACCCAAATCGCCCAA GCCTCAGAATGCAGTCACCATCGCTGTGTCCTCCCGAGCCTTGTTTCGCATGGACGAGGAGCAGCAGATC TACACGGAGCAGGGCGTGGAGGAGTACGTGCGCTACCAGCTGGAACATGAGAACGAACCCTTCAGTCCCG GGCCAGCCTTCCCTTTTGTGAAGGCTCTGGAGGCCGTGAACAGGCGGCTGCGGGAGCTGTACCCTGATAG TGAGGACGTCTTCGACATCGTCCTCATGACTAACAACCATGCTCAAGTGGGTGTCCGCCTCATCAACAGT ATCAACCACTATGACCTGTTCATCGAGAGGTTCTGCATGACAGGTGGGAACAGCCCGATCTGCTACCTCA AGGCCTATCACACCAACCTCTACTTGTCAGCCGATGCGGAAAAAGTGCGAGAAGCCATTGATGAGGGGAT CGCAGCTGCCACCATCTTCAGCCCCAGCAGGGATGTGGTTGTGTCCCAGAGTCAGCTGCGCGTGGCCTTC GATGGGGACGCCGTGCTCTTCTCGGACGAGTCGGAGCGCATCGTCAAGGCCCACGGGCTGGACCGATTCT TCGAGCATGAGAAGGCCCACGAGAACAAGCCTCTGGCTCAGGGCCCCTTAAAGGGCTTTCTGGAGGCACT GGGTAGGTTGCAGAAGAAGTTCTACTCCAAAGGCCTGCGGCTGGAGTGCCCAATTCGTACCTACTTGGTG ACAGCACGCAGTGCAGCCAGTTCCGGGGCCCGGGCTCTCAAGACCCTGCGCAGCTGGGGCCTGGAGACAG ATGAAGCCTTGTTCCTTGCTGGAGCGCCCAAGGGCCCTCTCCTTGAGAAGATCCGCCCACACATCTTCTT TGATGACCAGATGTTCCATGTGGCTGGGGCTCAGGAGATGGGCACTGTGGCCGCCCATGTGCCTTATGGT GTGGCACAGACACCCCGGCGGACTGCACCTGCAAAGCAGGCCCCATCTGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224617 representing NM_032526
Red=Cloning site Green=Tags(s) MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQNAVTIAVSSRALFRMDEEQQI YTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINS INHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAF DGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLV TARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYG VAQTPRRTAPAKQAPSAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_032526 |
| ORF Size | 1104 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032526.1, NP_115915.1 |
| RefSeq Size | 1107 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 84618 |
| UniProt ID | Q9BXI3 |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
| MW | 41.5 kDa |
| Gene Summary | Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224617L3 | Lenti-ORF clone of NT5C1A (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic IA (NT5C1A) |
CNY 5,890.00 |
|
| RC224617L4 | Lenti-ORF clone of NT5C1A (mGFP-tagged)-Human 5'-nucleotidase, cytosolic IA (NT5C1A) |
CNY 5,890.00 |
|
| RG224617 | NT5C1A (tGFP-tagged) - Human 5'-nucleotidase, cytosolic IA (NT5C1A) |
CNY 7,088.00 |
|
| SC305464 | NT5C1A (untagged)-Human 5'-nucleotidase, cytosolic IA (NT5C1A) |
CNY 5,488.00 |
