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SPIRE1 (NM_001128627) Human Tagged ORF Clone
CAT＃: RC226036
SPIRE1 (Myc-DDK-tagged)-Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3
ORF Plasmid: tGFP
"NM_001128627" in other vectors (4)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Spir-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226036 representing NM_001128627
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACAGAGGTCATTGAATCTTTGGGAATTATTATTTATAAAGCACTGGACTATGGTTTGAAGGAGA ATGAAGAAAGGGAATTAAGCCCTCCCCTAGAGCAGCTTATCGATCACATGGCCAACACGGTGGAAGCTGA CGGTAGCAATGATGAGGGCTATGAGGCTGCAGAAGAAGGCCTGGGAGATGAAGATGAAAAGAGAAAAATC TCAGCTATTCGGTCATATAGAGATGTCATGAAGTTGTGTGCTGCTCATCTCCCTACTGAATCAGATGCAC CAAATCATTATCAGGCAGTATGTCGTGCACTGTTTGCAGAAACAATGGAGCTCCATACATTTCTGACCAA AATTAAGAGTGCGAAAGAGAATCTTAAGAAGATTCAAGAAATGGAAAAGAGCGATGAATCTAGCACAGAC TTGGAAGAGCTGAAAAACGCTGACTGGGCACGATTCTGGGTACAGGTGATGAGGGATTTGAGGAATGGGG TAAAACTTAAGAAGGTCCAAGAGCGGCAGTACAACCCTTTGCCCATTGAATATCAGCTCACCCCTTATGA GATGTTAATGGATGACATTCGCTGCAAAAGATACACCTTGCGAAAAGTGATGGTGAATGGTGATATTCCC CCTCGGTTAAAAAAGAGTGCTCATGAAATCATCCTCGACTTCATCAGATCCAGACCTCCTTTAAATCCAG TCTCAGCCAGAAAACTGAAACCAACTCCACCACGGCCACGGAGCCTCCATGAAAGAATATTAGAAGAAAT TAAAGCAGAAAGAAAGCTGCGGCCTGTATCACCAGAGGAGATTAGACGTAGCAGATTAGATGTGACTACC CCTGAATCTACAAAGAATCTTGTGGAGTCATCTATGGTGAATGGAGGTTTGACATCACAAACAAAAGAAA ACGGGTTAAGTACCTCACAGCAGGTGCCTGCACAGCGGAAGAAGCTCCTCAGAGCCCCAACTCTGGCCGA ACTGGACAGCTCTGAGTCTGAGGAAGAAACGCTGCACAAGTCGACCAGCAGCAGCAGCGTGTCTCCCTCT TTCCCTGAAGAGCCAGTCCTGGAGGCCGTGTCCACAAGGAAGAAGCCTCCAAAATTCCTGCCCATATCAT CAACACCCCAGCCAGAGAGACGGCAGCCACCCCAGAGACGACATTCCATTGAAAAGGAAACGCCTACTAA CGTGAGGCAGTTCCTGCCGCCTTCCAGGCAGAGTTCCCGCTCTCTTGAGGAATTCTGCTACCCAGTGGAA TGCCTCGCTCTTACTGTGGAAGAAGTGATGCATATTCGCCAGGTCCTGGTGAAGGCAGAGCTGGAAAAAT ACCAACAGTATAAAGACATCTACACCGCCTTGAAAAAAGGAAAGCTCTGCTTTTGTTGCCGAACCAGGAG GTTTTCCTTCTTCACTTGGTCTTATACCTGTCAGTTCTGTAAGAGGCCGGTGTGCTCACAGTGTTGCAAA AAGATGCGGCTGCCCTCCAAACCATACTCCACTCTTCCTATCTTTTCATTGGGACCTTCTGCTCTGCAAA GAGGGGAAAGTAGTATGAGGTCAGAAAAACCCTCCACTGCCCATCATCGGCCACTTCGGAGCATTGCCAG GTTCTCCTCAAAATCTAAGTCTATGGACAAATCAGATGAAGAACTCCAGTTTCCCAAAGAGTTGATGGAG GACTGGAGCACCATGGAGGTGTGTGTGGACTGCAAGAAGTTCATTTCGGAAATCATCAGTTCAAGCCGGC GCAGTCTGGTGTTGGCCAACAAAAGGGCCCGATTGAAAAGGAAAACGCAGTCTTTCTACATGTCCTCGCC AGGCCCCTCGGAGTACTGCCCTTCAGAGAGGACGATCAGTGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226036 representing NM_001128627
Red=Cloning site Green=Tags(s) METEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYEAAEEGLGDEDEKRKI SAIRSYRDVMKLCAAHLPTESDAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDESSTD LEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIP PRLKKSAHEIILDFIRSRPPLNPVSARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVTT PESTKNLVESSMVNGGLTSQTKENGLSTSQQVPAQRKKLLRAPTLAELDSSESEEETLHKSTSSSSVSPS FPEEPVLEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVE CLALTVEEVMHIRQVLVKAELEKYQQYKDIYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCK KMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELME DWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001128627 |
| ORF Size | 1866 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001128627.1, NP_001122099.1 |
| RefSeq ORF | 1869 bp |
| Locus ID | 56907 |
| UniProt ID | Q08AE8 |
| Protein Pathways | Dorso-ventral axis formation |
| MW | 71.4 kDa |
| Gene Summary | Spire proteins, such as SPIRE1, are highly conserved between species. They belong to the family of Wiskott-Aldrich homology region-2 (WH2) proteins, which are involved in actin organization (Kerkhoff et al., 2001 [PubMed 11747823]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226036L3 | Lenti ORF clone of Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC226036L4 | Lenti ORF clone of Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3, mGFP tagged |
CNY 6,460.00 |
|
| RG226036 | SPIRE1 (tGFP-tagged) - Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3 |
CNY 5,040.00 |
|
| SC323156 | SPIRE1 (untagged)-Human spire homolog 1 (Drosophila) (SPIRE1), transcript variant 3 |
CNY 9,980.00 |
