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Gelsolin (GSN) (NM_001127663) Human Tagged ORF Clone
CAT＃: RC226118
- TrueORF®
GSN (Myc-DDK-tagged)-Human gelsolin (GSN), transcript variant 4
ORF Plasmid: tGFP
"NM_001127663" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ADF; AGEL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226118 representing NM_001127663
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATACAGGACTTACGCGTCTGCTGTGGCCCAGCTGGCTTCCAGATGGTGACATGAGCCACCCACAGC CGGAGCTGTTCCTCTTTCCCAAAGCTCAGCCCAACAGCATGGTGGTGGAACACCCCGAGTTCCTCAAGGC AGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGAAGTTCGATCTGGTGCCCGTGCCCACCAACCTT TATGGAGACTTCTTCACGGGCGACGCCTACGTCATCCTGAAGACAGTGCAGCTGAGGAACGGAAATCTGC AGTATGACCTCCACTACTGGCTGGGCAATGAGTGCAGCCAGGATGAGAGCGGGGCGGCCGCCATCTTTAC CGTGCAGCTGGATGACTACCTGAACGGCCGGGCCGTGCAGCACCGTGAGGTCCAGGGCTTCGAGTCGGCC ACCTTCCTAGGCTACTTCAAGTCTGGCCTGAAGTACAAGAAAGGAGGTGTGGCATCAGGATTCAAGCACG TGGTACCCAACGAGGTGGTGGTGCAGAGACTCTTCCAGGTCAAAGGGCGGCGTGTGGTCCGTGCCACCGA GGTACCTGTGTCCTGGGAGAGCTTCAACAATGGCGACTGCTTCATCCTGGACCTGGGCAACAACATCCAC CAGTGGTGTGGTTCCAACAGCAATCGGTATGAAAGACTGAAGGCCACACAGGTGTCCAAGGGCATCCGGG ACAACGAGCGGAGTGGCCGGGCCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCA GGTGCTGGGCCCCAAGCCGGCTCTGCCTGCAGGTACCGAGGACACCGCCAAGGAGGATGCGGCCAACCGC AAGCTGGCCAAGCTCTACAAGGTCTCCAATGGTGCAGGGACCATGTCCGTCTCCCTCGTGGCTGATGAGA ACCCCTTCGCCCAGGGGGCCCTGAAGTCAGAGGACTGCTTCATCCTGGACCACGGCAAAGATGGGAAAAT CTTTGTCTGGAAAGGCAAGCAGGCAAACACGGAGGAGAGGAAGGCTGCCCTCAAAACAGCCTCTGACTTC ATCACCAAGATGGACTACCCCAAGCAGACTCAGGTCTCGGTCCTTCCTGAGGGCGGTGAGACCCCACTGT TCAAGCAGTTCTTCAAGAACTGGCGGGACCCAGACCAGACAGATGGCCTGGGCTTGTCCTACCTTTCCAG CCATATCGCCAACGTGGAGCGGGTGCCCTTCGACGCCGCCACCCTGCACACCTCCACTGCCATGGCCGCC CAGCACGGCATGGATGACGATGGCACAGGCCAGAAACAGATCTGGAGAATCGAAGGTTCCAACAAGGTGC CCGTGGACCCTGCCACATATGGACAGTTCTATGGAGGCGACAGCTACATCATTCTGTACAACTACCGCCA TGGTGGCCGCCAGGGGCAGATAATCTATAACTGGCAGGGTGCCCAGTCTACCCAGGATGAGGTCGCTGCA TCTGCCATCCTGACTGCTCAGCTGGATGAGGAGCTGGGAGGTACCCCTGTCCAGAGCCGTGTGGTCCAAG GCAAGGAGCCCGCCCACCTCATGAGCCTGTTTGGTGGGAAGCCCATGATCATCTACAAGGGCGGCACCTC CCGCGAGGGCGGGCAGACAGCCCCTGCCAGCACCCGCCTCTTCCAGGTCCGCGCCAACAGCGCTGGAGCC ACCCGGGCTGTTGAGGTATTGCCTAAGGCTGGTGCACTGAACTCCAACGATGCCTTTGTTCTGAAAACCC CCTCAGCCGCCTACCTGTGGGTGGGTACAGGAGCCAGCGAGGCAGAGAAGACGGGGGCCCAGGAGCTGCT CAGGGTGCTGCGGGCCCAACCTGTGCAGGTGGCAGAAGGCAGCGAGCCAGATGGCTTCTGGGAGGCCCTG GGCGGGAAGGCTGCCTACCGCACATCCCCACGGCTGAAGGACAAGAAGATGGATGCCCATCCTCCTCGCC TCTTTGCCTGCTCCAACAAGATTGGACGTTTTGTGATCGAAGAGGTTCCTGGTGAGCTCATGCAGGAAGA CCTGGCAACGGATGACGTCATGCTTCTGGACACCTGGGACCAGGTCTTTGTCTGGGTTGGAAAGGATTCT CAAGAAGAAGAAAAGACAGAAGCCTTGACTTCTGCTAAGCGGTACATCGAGACGGACCCAGCCAATCGGG ATCGGCGGACGCCCATCACCGTGGTGAAGCAAGGCTTTGAGCCTCCCTCCTTTGTGGGCTGGTTCCTTGG CTGGGATGATGATTACTGGTCTGTGGACCCCTTGGACAGGGCCATGGCTGAGCTGGCTGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC226118 representing NM_001127663
Red=Cloning site Green=Tags(s) MNTGLTRLLWPSWLPDGDMSHPQPELFLFPKAQPNSMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNL YGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESA TFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNIH QWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANR KLAKLYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDF ITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAA QHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAA SAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGA TRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEAL GGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDS QEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001127663 |
| ORF Size | 2301 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127663.2 |
| RefSeq Size | 2797 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 2934 |
| UniProt ID | P06396 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| MW | 84.7 kDa |
| Gene Summary | The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226118L3 | Lenti-ORF clone of GSN (Myc-DDK-tagged)-Human gelsolin (GSN), transcript variant 4 |
CNY 5,890.00 |
|
| RC226118L4 | Lenti-ORF clone of GSN (mGFP-tagged)-Human gelsolin (GSN), transcript variant 4 |
CNY 5,890.00 |
|
| RG226118 | GSN (tGFP-tagged) - Human gelsolin (GSN), transcript variant 4 |
CNY 4,370.00 |
|
| SC323185 | GSN (untagged)-Human gelsolin (GSN), transcript variant 4 |
CNY 7,220.00 |
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