RGPD6 (NM_001123363) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RanBP2L1; RanBP2L2; RGP6; RGPD7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226429 representing NM_001123363 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGCAGCAAGGCCGATGTGGAGCGGTACGTCGCCTCGGTGCTGGGTCTCACCCCGTCGCCTCGAC AGAAGTCAATGAAAGGATTCTATTTTGCAAAGCTGTATTATGAAGCTAAAGAATATGATCTTGCTAAAAA ATACATATGTACTTACATTAATGTGCAAGAGAGGGATCCCAAAGCTCACAGATTTCTGGGTCTTCTTTAT GAATTGGAAGAAAACACAGAGAAAGCCGTTGAATGTTACAGGCGTTCAGTGGAATTAAACCCAACACAAA AAGATCTTGTGTTGAAGATTGCAGAATTGCTTTGTAAAAATGATGTTACTGATGGAAGAGCAAAATACTG GGTCGAAAGAGCAGCAAAACTTTTCCCAGGAAGTCCTGCAATTTATAAACTAAAGGAACAGCTTCTAGAT TGTGAAGGTGAAGATGGATGGAATAAACTTTTTGACTTGATTCAGTCAGAACTTTATGTAAGACCTGATG ACGTCCATGTGAACATCCGGCTAGTGGAGTTGTATCGCTCAACTAAAAGATTGAAGGATGCTGTGGCCCA CTGCCATGAGGCAGAGAGGAACATAGCTTTGCGTTCAAGTTTAGAGTGGAATTCGTGTGTTGTACAGACC CTTAAGGAATATCTGGAGTCTTTACAGTGTTTGGAGTCTGATAAAAGTGACTGGCAAGCAACCAATACAG ACTTACTGCTGGCCTATGCTAATCTTATGCTTCTTACGCTTTCCACTAGAGATGTGCAGGAAAATAGAGA ATTACTGGAAAGTTTTGATAGTGCTCTTCAGTCTGCGAAATCTTCTTTGGGTGGAAATGATGAACTGTCA GCTACTTTCTTAGAAATGAAAGGACATTTCTATATGTATGCTGGTTCTCTGCTCTTGAAGATGGGTCAGC ATGGTAATAATGTTCAATGGCGAGCTCTTTCTGAGCTGGCTGCATTGTGCTATCTCATAGCATTTCAGGT TCCAAGACCAAAGATTAAATTAAGAGAAGGTAAAGCTGGACAAAATCTGCTGGAAATGATGGCCTGTGAC CGACTGAGCCAATCAGGGCACATGTTGCTAAGCTTAAGTCGTGGCAAGCAAGATTTCTTAAAAGAGGTTG TTGAAACTTTTGCCAACAAAATTGGGCAGTCTGCGTTATATGATGCTCTGTTTTCTAGTCAGTCACCTAA GGATACATCTTTTCTTGGTAGCGATGATATTGGAAAAATTGATGTACAAGAACCAGAGCTTGAAGATTTG GCTAGATACGATGTTGGTGCTATTCGAGCACATAATGGTAGTCTTCAGCATCTTACTTGGCTTGGCTTAC AGTGGAATTCATTGCCTGCTTTACCTGGAATCCGAAAATGGCTAAAACAGCTTTTCCATCGTTTGCCCCA TGAAACCTCAAGGCTTGAAACAAATGCGCCTGAATCAATATGTATTTTAGATCTTGAAGTATTTCTCCTT GGAGTAGTATATACCAGCCACTTACAATTAAAGGAGAAATGTAATTCTCACCATAGCTCCTATCAGCCGT TATGCCTGCCCTTTCCTGTGTGTAAACAGCTTTGTACAGAAAGACAAAAATCTTGGTGGGATGCGGTTTG TACTCTGATTCACAGAAAAGCAGTACCTGGAAACTTGGCAAAATTGAGACTTCTAGTTCAGCATGAAATA AACACTCTAAGAGCCCAGGAAAAACATGGCCTTCAACCTGCTCTGCTTGTACATTGGGCAAAATACCTTC AGAAAACGGGCAGCGGTCTTAATTCTTTTTATGGTCAACTAGAATACATAGGGAGAAGTGTTCATTATTG GAAGAAAGTTTTGCCATTGTTGAAGATAATAAAGAAGAACAGTATTCCTGAACCTATTGATCCTCTGTTT AAACATTTTCATAGTGTAGACATTCAGGCATCAGAAATTGTTGAATATGAAGAAGACGCACACATAACTT TTGCTATGTTGGATGCAGTAAATGGAAATATAGAAGATGCTGTGACTGCTTTTGAATCTATAAAAAGTGT TGTTTCTTATTGGAATCTTGCACTGATTTTTCACAGGAAGGCAGAAGACATTGAAAATGATGCCCTTTCT CCTGAAGAACAAGAAGAATGCAGAAATTATCTGACAAAGACCAGGGACTACCTAATAAAGATTATAGATG ACGGTGATTCAAATCTTTCAGTGGTCAAGAAATTGCCTGTGCCCCTGGAGTCTGTAAAACAGATGCTTAA TTCAGTCATGCAGGAACTCGAAGACTATAGTGAAGGAGGTCCTCTCTATAAAAATGGTTCTTTGCGAAAT GCAGATTCAGAAATAAAACATTCTACACCGTCTCCTACCAAATATTCACTATCACCAAGTAAAAGTTACA AGTATTCTCCCGAAACACCACCTCGATGGACAGAAGATCGGAATTCTTTACTGAATATGATTTGCCAACA AGTAGAGGCCATTAAGAAAGAAATGCAGGAGTTGAAACTAAATAGCAGTAAGTCAGCATCCCGTCATCGT TGGCCCACAGAGAATTATGGACCAGACTCGGTGCCTGATGGATATCAGGGGTCACAGACATTTCATGGGG CTCCACTAACAGTTGCAACTACTGGCCCTTCAGTATATTATAGTCAGTCACCAGCATATAATTCCCAGTA TCTTCTCAGACCAGCAGCTAATGTTACTCCCACAAAGGGTTCTTCTAATACAGAATTTAAGTCAACCAAA GAAGGATTTTCCATCCCTGTGTCTGCTGATGGATTTAAATTTGGCATTTCGGAACCAGGAAATCAAGAAA AGAAAAGGGAAAAGCCTCTTGAAAATGATACTGGCTTCCAGGCTCAGGATATTAGTGGCCGGAAGAAGGG CCGTGGTGTGATTTTTGGCCAAACAAGTAGCACTTTTACATTTGCAGATGTTGCAAAATCAACTTCAGGA GAAGGATTTCAGTTTGGCAAAAAAGACCTCAATTTCAAGGGATTTTCAGGTGCTGGAGAAAAATTATTCT CATCACGATACGGTAAAATGGCCAATAAAGCAAACACTTCCGGTGACTTTGAGAAAGATGATGATGCCTA TAAGACTGAGGACAGCGATGACATCCATTTTGAACCAGTAGTTCAAATGCCTGAAAAAGTAGAACTTGTA ACAGGAGAAGAAGGTGAAAAAGTTCTGTATTCACAGGGGGTAAAACTATTTAGATTTGATGCTGAGGTAA GGCAGTGGAAAGAAAGGGGCTTGGGGAACTTAAAAATTCTCAAAAACGAGGTCAATGGCAAACTAAGAAT GCTGATGCGAAGAGAACAAGTACTAAAAGTGTGTGCTAATCATTGGATAACGACTACAATGAACCTGAAG CCCCTCTCTGGATCAGATAGAGCATGGATGTGGTCAGCCAGTGATTTCTCTGACGGTGATGCCAAACTAG AGCGGTTGGCAGCAAAATTTAAAACACCAGAGCTGGCTGAAGAATTCAAGCAGAAATTTGAGGAATGCCA GCGGCTTCTGTTAGACATACCACTTCAAACTCCCCATAAACTTGTAGATACTGGCAGAGCTGCCAAGTTA ATACAGAGAGCTGAAGAAATGAAGAGTGGACTGAAAGATTTCAAAACATTTTTGACAAATGATCAAACAA AAGTCACTGAGGAAGAAAATAAGGGTTCAGGTACAGGTGCGGCCGGTGCCTCAGACACAACAATAAAACC CAATGCTGAAAACACTGGGCCCACATTAGAATGGGATAACTATGACTTAAGGGAAGATGCTTTGGATGAT AGTGTCAGTAGTAGCTCAGTACATGCTTCTCCATTGGCAAGTAGCCCTGTGAGAAAAAATCTTTTCCGCT TTGATGAGTCAACAACAGGATCTAACTTCAGTTTTAAATCTGCTTTGAGTCTATCTAAGTCTCCTGCCAA GTTGAATCAGAGTGGGACTTCAGTTGGCACTGATGAAGAATCTGTTGTTACTCAAGAAGAAGAGAGAGAT GGACAGTACTTTGAACCTGTTGTTCCTTTACCTGATCTAGTTGAAGTATCCAGTGGTGAGGAAAATGAAC AAGTTGTTTTTAGTCACAGGGCAGAAATCTACAGATATGATAAAGATGTTGGTCAATGGAAAGAAAGGGG CATTGGTGATATAAAGATTTTACAGAATTATGATAATAAGCAAGTTCGTATAGTGATGAGAAGGGACCAA GTATTAAAACTTTGTGCCAATCACAGAATAACTCCAGACATGAGTTTGCAAAATATGAAAGGGACAGAAA GAGTATGGGTGTGGACTGCATGTGATTTTGCAGATGGAGAAAGAAAAGTAGAGCATTTAGCTGTTCGTTT TAAACTACAGGATGTTGCAGACTCGTTTAAGAAAATTTTTGATGAAGCAAAAACAGCCCAGGAAAAAGAT TCTTTGATAACACCTCATGTTTCTCGGTCAAGCACTCCCAGAGAGTCACCATGTGGCAAAATTGCTGTAG CTATATTAGAAGAAACCACAAGAGAGAGGACAGATGTTATTCAGGGTGATGATGTAGCAGATGCAGCTTC AGAAGTTGAAGTGTCTAGCACATCTGAAACAACAACAAAAGCAGTGGTTTCTCCTCCAAAGTTTGTATTT GTTTCAGAGTCTGTTAAAAGAATTTTTAGTAGTGAAAAATCAAAACCATTTGTATTTGGCAACAGTTCTG CCACTGGGTCTTTGTTTGGATTTAGTTTTAATGCACCTTTGAAAAGTAACAATAGTGAAACTAGTTCAGT AGCCCAGAGTGGATCTGAAAGCAAAGTGGAACCTAAAAAATGTGAACTGTCAAAGAACTCTGATATCGAA CAGTCTTCAGATAGCAAAGTCAAAAATCTCTCTGCTTCCTTTCCAACGGAAGAATCTTCAATCAACTACA CATTTAAAACACCAGAAAAGGAGCCTCCATTATGGCATGCTGAATTTACCAAAGAAGAATTGGTTCAGAA GCTCCGTTCCACCACAAAAAGTGCAGATCACTTAAACGGCCTGCTTCGGGAAATAGAGGCAACCAATGCA GTCCTTATGGAGCAAATTAAGCTTCTCAAAAGTGAAATAAGAAGATTGGAAAGGAATCAAGAGCGAGAGA AGTCTGCAGCTAACCTGGAATACTTGAAGAACGTCTTGCTGCAGTTCATTTTCTTGAAGCCAGGTAGTGA AAGAGAGAGACTTCTTCCTGTTATAAATACGATGTTGCAGCTCAGCCCTGAAGAAAAGGGAAAACTTGCT GCGGTTGCTCAAGATGAGGAAGAAAATGCTTCCCGTTCTTCTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226429 representing NM_001123363 Red=Cloning site Green=Tags(s) MRRSKADVERYVASVLGLTPSPRQKSMKGFYFAKLYYEAKEYDLAKKYICTYINVQERDPKAHRFLGLLY ELEENTEKAVECYRRSVELNPTQKDLVLKIAELLCKNDVTDGRAKYWVERAAKLFPGSPAIYKLKEQLLD CEGEDGWNKLFDLIQSELYVRPDDVHVNIRLVELYRSTKRLKDAVAHCHEAERNIALRSSLEWNSCVVQT LKEYLESLQCLESDKSDWQATNTDLLLAYANLMLLTLSTRDVQENRELLESFDSALQSAKSSLGGNDELS ATFLEMKGHFYMYAGSLLLKMGQHGNNVQWRALSELAALCYLIAFQVPRPKIKLREGKAGQNLLEMMACD RLSQSGHMLLSLSRGKQDFLKEVVETFANKIGQSALYDALFSSQSPKDTSFLGSDDIGKIDVQEPELEDL ARYDVGAIRAHNGSLQHLTWLGLQWNSLPALPGIRKWLKQLFHRLPHETSRLETNAPESICILDLEVFLL GVVYTSHLQLKEKCNSHHSSYQPLCLPFPVCKQLCTERQKSWWDAVCTLIHRKAVPGNLAKLRLLVQHEI NTLRAQEKHGLQPALLVHWAKYLQKTGSGLNSFYGQLEYIGRSVHYWKKVLPLLKIIKKNSIPEPIDPLF KHFHSVDIQASEIVEYEEDAHITFAMLDAVNGNIEDAVTAFESIKSVVSYWNLALIFHRKAEDIENDALS PEEQEECRNYLTKTRDYLIKIIDDGDSNLSVVKKLPVPLESVKQMLNSVMQELEDYSEGGPLYKNGSLRN ADSEIKHSTPSPTKYSLSPSKSYKYSPETPPRWTEDRNSLLNMICQQVEAIKKEMQELKLNSSKSASRHR WPTENYGPDSVPDGYQGSQTFHGAPLTVATTGPSVYYSQSPAYNSQYLLRPAANVTPTKGSSNTEFKSTK EGFSIPVSADGFKFGISEPGNQEKKREKPLENDTGFQAQDISGRKKGRGVIFGQTSSTFTFADVAKSTSG EGFQFGKKDLNFKGFSGAGEKLFSSRYGKMANKANTSGDFEKDDDAYKTEDSDDIHFEPVVQMPEKVELV TGEEGEKVLYSQGVKLFRFDAEVRQWKERGLGNLKILKNEVNGKLRMLMRREQVLKVCANHWITTTMNLK PLSGSDRAWMWSASDFSDGDAKLERLAAKFKTPELAEEFKQKFEECQRLLLDIPLQTPHKLVDTGRAAKL IQRAEEMKSGLKDFKTFLTNDQTKVTEEENKGSGTGAAGASDTTIKPNAENTGPTLEWDNYDLREDALDD SVSSSSVHASPLASSPVRKNLFRFDESTTGSNFSFKSALSLSKSPAKLNQSGTSVGTDEESVVTQEEERD GQYFEPVVPLPDLVEVSSGEENEQVVFSHRAEIYRYDKDVGQWKERGIGDIKILQNYDNKQVRIVMRRDQ VLKLCANHRITPDMSLQNMKGTERVWVWTACDFADGERKVEHLAVRFKLQDVADSFKKIFDEAKTAQEKD SLITPHVSRSSTPRESPCGKIAVAILEETTRERTDVIQGDDVADAASEVEVSSTSETTTKAVVSPPKFVF VSESVKRIFSSEKSKPFVFGNSSATGSLFGFSFNAPLKSNNSETSSVAQSGSESKVEPKKCELSKNSDIE QSSDSKVKNLSASFPTEESSINYTFKTPEKEPPLWHAEFTKEELVQKLRSTTKSADHLNGLLREIEATNA VLMEQIKLLKSEIRRLERNQEREKSAANLEYLKNVLLQFIFLKPGSERERLLPVINTMLQLSPEEKGKLA AVAQDEEENASRSSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001123363
ORF Size	5295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001123363.3, NP_001116835.1
RefSeq ORF	5298 bp
Locus ID	729540
UniProt ID	Q99666
Protein Families	Druggable Genome
MW	198.7 kDa

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