ZNF853 (NM_017560) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC227566 ORF sequence, codon optimized. Due to the complexity of NM_017560, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCACCAGCCTACCCCAGGCAACAGAGGCCTGACTGCAAGAATGGAGGTGGGACCCGCAACCGAGA CATTTGTGTTGGAACTGCAGTGCCTGGAGGATGGCGGACCTGGGCCCGATACGCTGAGTGGGGGTTCTGG GGGGAGCGAGTCTCAGGAGGAGGAAGAACCCCAAGAAAGAAATAGTAGCCCGCAGAGGCCCGCCGTCTCA GCACCGGTTGGCGCTAGCGAGATCGCGGAAGAAACTCGGCCCGGGCAGAGAGAGCTCCAACTCCAGCAGC TTGAACAACAGCCCGAACCCCAGCAGCAGCCGCAGCACGAGCAGCTCCAACAACCACAGCCCCATCTGGA GCTTCAACAGCAGCCTCAACAGGATGGCCAGCAGCAACTTTCACAGCTTCAGCAAGAGAAGCACCAGTCA GTCCACCATCAGGAGCTTAAGCCCGAACTGCAGCTGATGCATCAACAGCAGCAGCTGCAGCCTCAGCAGG TTCAGGAACAGCAGCGGTTGCAGCAGCAGCAGGAACAGCTTCAGACACAGCAGGCTCAGGAGCAGCAGGT CCTTCAGCAGCAGGAGCAATTGCAACAGCAGGTCCAGGAACAGCAGCTGCTTCAGCAGCAGCAGGAGCAA CTGCAACAGCAACAGCTGTTGCAGCAGCAGGAGCAGCTGCAGCAGCAGCAATTTCAGCAGCAGCAGGAGC AACTCCAACAGCAGCAGCAGCTGTTGTTGCTCCAGCAGCAGGGACAACTGCAGCAGCAGCTGCTTCAGCA GCAGCAGGCTCAACTGCAGCAACAGCTCCTGGAGCAGCAACAGGCCCAGCTGCAGCAGCAACTGCTGTTG CAGCAGCAAGAGCAGCTGCAGCAGCAGCAGCAGCAGCAGCTCCTGCAGCAGCAGCAAGAACAGCTGCAGC AGCAGCAGCTGCAACCCCCACCCTTGGAACCAGAAGAGGAGGAGGAAGTTGAATTGGAACTGATGCCAGT TGATCTGGGCTCCGAACAGGAGTTGGAACAGCAGAGACAAGAGTTGGAAAGACAACAGGAACTGGAGAGA CAACAGGAGCAGAGGCAGCTCCAACTGAAGTTGCAAGAAGAGCTGCAGCAGCTGGAGCAGCAGCTTGAAC AGCAGCAGCAGCAACTGGAGCAGCAAGAGGTCCAGTTGGAACTTACTCCTGTCGAGCTCGGCGCACAGCA GCAGGAGGTCCAGCTGGAGCTGACTCCCGTGCAGCCCGAACTCCAGCTGGAGTTGGTTCCTGCAGCTGGT GGCGGCGGCGCCGCTGTGCCTGGCGCCCCCGCAGCTGTTGTGGTTGCCCCACCAGGGTATGTGGTTGTTC AGGAACTTATGGTACTGCCCGCTGTAGCTGCGCCTGCCGTTGTGGCAATCCCTGGCCCAGCTGGCTCCGC TGCCCTTACCCCCGCTCGCCAACGCCGGCGCCGCAGAGCTAGAGATCGACCTACTATATGCGGTGAGTGC GGGAAAGGGTTCTCCAGATCAACAGACCTCGTGCGACATCAGGCCACGCACACTGGTGAGAGGCCTCATC GATGCGGCGAATGCGGCAAGGGGTTTTCTCAGCACAGCAACCTGGTGACTCACCAGAGGATCCACACCGG AGAGAAACCATACGCCTGCAGCTACTGCGCCAAGAGGTTTAGCGAGTCATCAGCTCTCGTGCAGCACCAG CGGACCCACACAGGCGAAAGGCCCTACGCATGCGGTGATTGTGGAAAGCGATTTTCCGTAAGTTCTAACC TGCTGCGCCACCGGAGAACCCACAGCGGCGAGCGGCCCTATGTGTGCGAGGATTGTGGAGAGCGATTCCG ACATAAGGTCCAGATACGGAGGCACGAGCGGCAACTCCATGGGGCGGGAAGATCTCGCGGTCTGGGACTG CTCCGCGCGTCAAGACCAGCCGCTCTGGGTGGCCCGGCCAGGGCCGAGCAAGCTGCAACCGCCACTGCAC CGGCCGATAAGGCGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC227566 representing NM_017560 Red=Cloning site Green=Tags(s) MLHQPTPGNRGLTARMEVGPATETFVLELQCLEDGGPGPDTLSGGSGGSESQEEEEPQERNSSPQRPAVS APVGASEIAEETRPGQRELQLQQLEQQPEPQQQPQHEQLQQPQPHLELQQQPQQDGQQQLSQLQQEKHQS VHHQELKPELQLMHQQQQLQPQQVQEQQRLQQQQEQLQTQQAQEQQVLQQQEQLQQQVQEQQLLQQQQEQ LQQQQLLQQQEQLQQQQFQQQQEQLQQQQQLLLLQQQGQLQQQLLQQQQAQLQQQLLEQQQAQLQQQLLL QQQEQLQQQQQQQLLQQQQEQLQQQQLQPPPLEPEEEEEVELELMPVDLGSEQELEQQRQELERQQELER QQEQRQLQLKLQEELQQLEQQLEQQQQQLEQQEVQLELTPVELGAQQQEVQLELTPVQPELQLELVPAAG GGGAAVPGAPAAVVVAPPGYVVVQELMVLPAVAAPAVVAIPGPAGSAALTPARQRRRRRARDRPTICGEC GKGFSRSTDLVRHQATHTGERPHRCGECGKGFSQHSNLVTHQRIHTGEKPYACSYCAKRFSESSALVQHQ RTHTGERPYACGDCGKRFSVSSNLLRHRRTHSGERPYVCEDCGERFRHKVQIRRHERQLHGAGRSRGLGL LRASRPAALGGPARAEQAATATAPADKAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017560
ORF Size	1977 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017560.1, NP_060030.1
RefSeq Size	3864 bp
RefSeq ORF	1980 bp
Locus ID	54753
UniProt ID	P0CG23
MW	74.9 kDa

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