United States
Germany
Japan
United Kingdom
China
TKTL1 (NM_001145933) Human Tagged ORF Clone
CAT＃: RC228477
TKTL1 (Myc-DDK-tagged)-Human transketolase-like 1 (TKTL1), transcript variant 2
ORF Plasmid: tGFP
"NM_001145933" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,392.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TKR; TKT2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC228477 representing NM_001145933
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATGCTGAGGCGAGGGCTGAGTTCCCGGAGGAGGCCAGACCTGACAGGGGCACCTTGCAGGTGT TGCAAGATATGGCCAGCCGCTTGCGAATCCATTCCATCAGGGCCACATGCTCCACGAGCTCCGGCAGTTC TTCTGAGATCATGTCTGTGCTGTTCTTCTACATCATGAGGTACAAGCAGTCAGATCCAGAGAATCCGGAC AACGACCGATTTGTCCTCGCAAAGAGACTGTCGTTTGTGGATGTGGCAACAGGATGGCTCGGACAAGGAC TGGGAGTTGCATGTGGAATGGCATATACTGGCAAGTACTTCGACAGGGCCAGCTACCGGGTGTTCTGCCT CATGAGTGATGGCGAGTCCTCAGAAGGCTCTGTCTGGGAGGCAATGGCCTTTGCTTCCTACTACAGTCTG GACAATCTTGTGGCAATCTTTGATGTGAACCGCCTGGGACACAGTGGTGCATTGCCCGCCGAGCACTGCA TAAACATCTATCAGAGGCGCTGCGAAGCCTTTGGGTGGAACACTTATGTGGTGGACGGCCGGGACGTGGA GGCACTGTGCCAGGTATTCTGGCAGGCTTCTCAGGTGAAGCACAAGCCCACTGCTGTGGTGGCCAAGACC TTCAAGGGCCGGGGCACCCCAAGTATTGAGGATGCAGAAAGTTGGCATGCAAAGCCAATGCCGAGAGAAA GAGCAGATGCCATTATCAAATTAATTGAGAGCCAGATACAGACCAGCAGGAATCTTGACCCACAGCCCCC CATTGAGGACTCACCTGAAGTCAACATCACAGATGTAAGGATGACCTCTCCACCTGATTACAGAGTTGGT GACAAGATAGCTACTCGGAAAGCATGCGGTCTGGCTCTGGCTAAGCTGGGCTACGCGAACAACAGAGTCG TTGTGCTGGATGGTGACACCAGGTACTCTACTTTCTCTGAGATATTCAACAAGGAGTACCCTGAGCGCTT CATCGAGTGCTTTATGGCTGAACAAAACATGGTGAGCGTGGCTCTGGGCTGTGCCTCCCGTGGACGGACC ATTGCTTTTGCTAGCACCTTTGCTGCCTTTCTGACTCGAGCATTTGATCACATCCGGATAGGAGGCCTCG CTGAGAGCAACATCAACATTATTGGTTCCCACTGTGGGGTATCTGTTGGTGACGATGGTGCTTCCCAGAT GGCCCTGGAGGATATAGCCATGTTCCGAACCATTCCCAAGTGCACGATCTTCTACCCAACTGATGCCGTC TCCACGGAGCATGCTGTTGCTCTGGCAGCCAATGCCAAGGGGATGTGCTTCATTCGGACCACCCGACCAG AAACTATGGTTATTTACACCCCACAAGAACGCTTTGAGATCGGACAGGCCAAGGTCCTCCGCCACTGTGT CAGTGACAAGGTCACAGTTATTGGAGCTGGAATTACTGTGTATGAAGCCTTAGCAGCTGCTGATGAGCTT TCGAAACAAGATATTTTTATCCGTGTCATCGACCTGTTTACCATTAAACCTCTGGATGTCGCCACCATCG TCTCCAGTGCAAAAGCCACAGAGGGCCGGATCATTACAGTGGAGGATCACTACCCGCAAGGTGGCATCGG GGAAGCTGTCTGCGCAGCCGTCTCCATGGATCCTGACATTCAGGTTCATTCGCTGGCAGTGTCGGGAGTG CCCCAGAGTGGGAAGTCCGAGGAATTGCTGGATATGTATGGAATTAGTGCCAGACATATCATAGTGGCCG TGAAATGCATGTTGCTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC228477 representing NM_001145933
Red=Cloning site Green=Tags(s) MADAEARAEFPEEARPDRGTLQVLQDMASRLRIHSIRATCSTSSGSSSEIMSVLFFYIMRYKQSDPENPD NDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAFASYYSL DNLVAIFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPTAVVAKT FKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSPPDYRVG DKIATRKACGLALAKLGYANNRVVVLDGDTRYSTFSEIFNKEYPERFIECFMAEQNMVSVALGCASRGRT IAFASTFAAFLTRAFDHIRIGGLAESNINIIGSHCGVSVGDDGASQMALEDIAMFRTIPKCTIFYPTDAV STEHAVALAANAKGMCFIRTTRPETMVIYTPQERFEIGQAKVLRHCVSDKVTVIGAGITVYEALAAADEL SKQDIFIRVIDLFTIKPLDVATIVSSAKATEGRIITVEDHYPQGGIGEAVCAAVSMDPDIQVHSLAVSGV PQSGKSEELLDMYGISARHIIVAVKCMLLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145933 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145933.2 |
| RefSeq ORF | 1773 bp |
| Locus ID | 8277 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Pentose phosphate pathway |
| MW | 64.5 kDa |
| Gene Summary | The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228477L3 | Lenti-ORF clone of TKTL1 (Myc-DDK-tagged)-Human transketolase-like 1 (TKTL1), transcript variant 2 |
CNY 7,130.00 |
|
| RC228477L4 | Lenti-ORF clone of TKTL1 (mGFP-tagged)-Human transketolase-like 1 (TKTL1), transcript variant 2 |
CNY 7,130.00 |
|
| RG228477 | TKTL1 (tGFP-tagged) - Human transketolase-like 1 (TKTL1), transcript variant 2 |
CNY 5,700.00 |
|
| SC327112 | TKTL1 (untagged)-Human transketolase-like 1 (TKTL1) transcript variant 2 |
CNY 9,600.00 |
