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ZFYVE27 (NM_001174122) Human Tagged ORF Clone
CAT＃: RC229830
ZFYVE27 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 7
ORF Plasmid: tGFP
"NM_001174122" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PROTRUDIN; SPG33 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC229830 representing NM_001174122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTTCTATGGGGCT CTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGCACGC TCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGATGAA CCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCTGATG GACAGCACGCCTGCCCTCACACCCACGGAGGACCTCACACCGGGCAGCGTGGAGGAGGCTGAGGAGGCTG AGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGGATGATGAGGGCGCCCCGTGCCCAGCAGAGGATGA GCTGGCCCTGCAGGACAACGGGTTCCTGAGCAAGAATGAGGTGCTGCGCAGCAAGGTGTCTCGGCTCACG GAGCGGCTCCGCAAGCGCTACCCCACCAACAACTTCGGGAACTGCACGGGCTGCTCGGCCACCTTCTCAG TGCTGAAGAAGAGGCGGAGCTGCAGTAATTGTGGAAACAGCTTCTGCTCTCGATGCTGCTCCTTCAAGGT GCCCAAGTCCTCCATGGGGGCCACAGCCCCTGAAGCCCAGAGGGAGACTGTGTTTGTGTGTGCCTCGTGT AACCAGACCTTGAGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC229830 representing NM_001174122
Red=Cloning site Green=Tags(s) MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRFYGA LLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLM DSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLT ERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASC NQTLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001174122 |
| ORF Size | 858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001174122.1, NP_001167593.1 |
| RefSeq ORF | 861 bp |
| Locus ID | 118813 |
| UniProt ID | Q5T4F4 |
| Protein Families | Transmembrane |
| MW | 32.1 kDa |
| Gene Summary | This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229830L3 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 7, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229830L4 | Lenti ORF clone of Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 7, mGFP tagged |
CNY 5,890.00 |
|
| RG229830 | ZFYVE27 (tGFP-tagged) - Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 7 |
CNY 4,370.00 |
|
| SC328468 | ZFYVE27 (untagged)-Human zinc finger FYVE domain containing 27 (ZFYVE27) transcript variant 7 |
CNY 6,270.00 |
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