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CARD8 (NM_001184900) Human Tagged ORF Clone
CAT＃: RC230245
CARD8 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 8 (CARD8), transcript variant 1
ORF Plasmid: tGFP
"NM_001184900" in other vectors (4)
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CNY 4,000.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230245 representing NM_001184900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGGGACAGTGGAT CCAGTAGGAACATAGATGCATCCAAACTCATTAGACTACAAGGATCACGGAAACTGTTGGTTGACAATAG CATACGGGAACTGCAATACACAAAAACTGGAATTTTTTTTCAGGCTGAGGCCTGTGTGACAAATGATACG GTATACAGGGAGCTACCCTGTGTTTCTGAGACCCTTTGTGACATCTCACATTTTTTCCAAGAAGATGATG AGACAGAGGCAGAGCCATTATTGTTCCGTGCTGTTCCTGAGTGTCAACTATCTGGGGGGGACATTCCCAG TGTATCAGAAGAGCAGGAATCTTCAGAGGGACAAGATTCAGGAGACATTTGCTCAGAAGAGAATCAAATA GTTTCCTCTTATGCTTCTAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATCGTCAGTTTCTGGGGC CTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACAGATACAGCGTTTGGTTCCCCACTGC TGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCTTCCTGGTAAGGGATGAGGTCACAGTGACGATTGCG TTTGGTTCCTGGAGTCAGCACCTGGCCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCT TGTTTGATGTCACTGCAGAGCCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCA AGCAGGTGAGGTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGGAGCAT CCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGATGGGCATCCTGCTGC GGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACACATTGATCTATTATCACCCCCACCC CGAAGATATTAAGTTCCACTTGTACCTTGTCCCCAGCGACGCCTTGCTAACAAAGGCGATAGATGATGAG GAAGATCGCTTCCATGGTGTGCGCCTGCAGACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTT ATATTGTGTCTAATTCTGCTAACCTGAAAGTAATGCCCAAGGAGTTGAAATTGTCCTACAGGAGCCCTGG AGAAATTCAGCACTTCTCAAAATTCTATGCTGGGCAGATGAAGGAACCCATTCAACTTGAGATTACTGAA AAAAGACATGGGACTTTGGTGTGGGATACTGAGGTGAAGCCAGTGGATCTCCAGCTTGTAGCTGCATCAG CCCCTCCTCCTTTCTCAGGTGCAGCCTTTGTGAAGGAGAACCACCGGCAACTCCAAGCCAGGATGGGGGA CCTGAAAGGGGTGCTCGATGATCTCCAAGACAATGAGGTTCTTACTGAGAATGAGAAGGAGCTGGTGGAG CAGGAAAAGACACGGCAGAGCAAGAATGAGGCCTTGCTGAGCATGGTGGAGAAGAAAGGGGACCTGGCCC TGGACGTGCTCTTCAGAAGCATTAGTGAAAGGGACCCTTACCTCGTGTCCTATCTTAGACAGCAGAATTT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230245 representing NM_001184900
Red=Cloning site Green=Tags(s) MEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLVDNSIRELQYTKTGIFFQAEACVTNDT VYRELPCVSETLCDISHFFQEDDETEAEPLLFRAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQI VSSYASKVCFEIEEDYKNRQFLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIA FGSWSQHLALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHFISLQAGEVDVSWFLVAHFKNEGMVLEH PARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHLYLVPSDALLTKAIDDE EDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKELKLSYRSPGEIQHFSKFYAGQMKEPIQLEITE KRHGTLVWDTEVKPVDLQLVAASAPPPFSGAAFVKENHRQLQARMGDLKGVLDDLQDNEVLTENEKELVE QEKTRQSKNEALLSMVEKKGDLALDVLFRSISERDPYLVSYLRQQNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001184900 |
| ORF Size | 1611 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001184900.3 |
| RefSeq ORF | 1614 bp |
| Locus ID | 22900 |
| UniProt ID | Q9Y2G2 |
| Protein Families | Druggable Genome |
| Protein Pathways | NOD-like receptor signaling pathway |
| MW | 61.1 kDa |
| Gene Summary | The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Loss-of-function CARD8 mutation causes NLRP3 inflammasome activation and Crohn's disease
,Mao, L;Kitani, A;Similuk, M;Oler, AJ;Albenberg, L;Kelsen, J;Aktay, A;Quezado, M;Yao, M;Montgomery-Recht, K;Fuss, IJ;Strober, W;,
J. Clin. Invest.
,PubMed ID 29408806
[CARD8]
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Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230245L3 | Lenti ORF clone of Human caspase recruitment domain family, member 8 (CARD8), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC230245L4 | Lenti ORF clone of Human caspase recruitment domain family, member 8 (CARD8), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG230245 | CARD8 (tGFP-tagged) - Human caspase recruitment domain family, member 8 (CARD8), transcript variant 1 |
CNY 5,040.00 |
|
| SC328883 | CARD8 (untagged)-Human caspase recruitment domain family member 8 (CARD8) transcript variant 1 |
CNY 4,008.00 |
