United States
Germany
Japan
United Kingdom
China
PGM1 (NM_001172818) Human Tagged ORF Clone
CAT＃: RC230308
PGM1 (Myc-DDK-tagged)-Human phosphoglucomutase 1 (PGM1), transcript variant 2
ORF Plasmid: tGFP
"NM_001172818" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,320.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDG1T; GSD14 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230308 representing NM_001172818
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGATTTTGAAGAATGGATTTCTGGGACATATAGAAAAATGGAAGAAGGTCCTCTCCCTCTGTTGA CTTTTGCTACAGCTCCCTACCACGATCAGAAACCAGGAACAAGTGGATTACGGAAGAAAACCTATTATTT TGAGGAAAAGCCATGCTATCTGGAGAATTTCATCCAGAGTATATTCTTTTCCATAGACCTAAAAGATCGC CAGGGATCATCACTGGTGGTTGGTGGAGATGGGCGGTACTTTAATAAATCAGCAATAGAAACAATAGTGC AGATGGCAGCTGCCAATGGGATCGGTCGCTTGGTTATCGGACAGAATGGAATCCTCTCCACCCCTGCTGT ATCCTGCATCATTAGAAAAATCAAAGCCATTGGTGGGATCATTCTGACAGCCAGTCACAACCCAGGGGGC CCCAATGGAGATTTTGGAATCAAATTCAATATTTCTAATGGAGGTCCTGCTCCAGAAGCAATAACTGATA AAATTTTCCAAATCAGCAAGACAATTGAAGAATATGCAGTTTGCCCTGACCTGAAAGTAGACCTTGGTGT TCTGGGAAAGCAGCAGTTTGACTTGGAAAATAAGTTCAAACCCTTCACAGTGGAAATTGTGGATTCGGTA GAAGCTTATGCTACAATGCTGAGAAGCATCTTTGATTTCAGTGCACTGAAAGAACTACTTTCTGGGCCAA ACCGACTGAAGATCCGTATTGATGCTATGCATGGAGTTGTGGGACCGTATGTAAAGAAGATCCTCTGTGA AGAACTCGGTGCCCCTGCGAACTCGGCAGTTAACTGCGTTCCTCTGGAGGACTTTGGAGGCCACCACCCT GACCCCAACCTCACCTATGCAGCTGACCTGGTGGAGACCATGAAGTCAGGAGAGCATGATTTTGGGGCTG CCTTTGATGGAGATGGGGATCGAAACATGATTCTGGGCAAGCATGGGTTCTTTGTGAACCCTTCAGACTC TGTGGCTGTCATTGCTGCCAACATCTTCAGCATTCCGTATTTCCAGCAGACTGGGGTCCGCGGCTTTGCA CGGAGCATGCCCACGAGTGGTGCTCTGGACCGGGTGGCTAGTGCTACAAAGATTGCTTTGTATGAGACCC CAACTGGCTGGAAGTTTTTTGGGAATTTGATGGACGCGAGCAAACTGTCCCTTTGTGGGGAGGAGAGCTT CGGGACCGGTTCTGACCACATCCGTGAGAAAGATGGACTGTGGGCTGTCCTTGCCTGGCTCTCCATCCTA GCCACCCGCAAGCAGAGTGTGGAGGACATTCTCAAAGATCATTGGCAAAAGTATGGCCGGAATTTCTTCA CCAGGTATGATTACGAGGAGGTGGAAGCTGAGGGCGCAAACAAAATGATGAAGGACTTGGAGGCCCTGAT GTTTGATCGCTCCTTTGTGGGGAAGCAGTTCTCAGCAAATGACAAAGTTTACACTGTGGAGAAGGCCGAT AACTTTGAATACAGCGACCCAGTGGATGGAAGCATTTCAAGAAATCAGGGCTTGCGCCTCATTTTCACAG ATGGTTCTCGAATCGTCTTCCGACTGAGCGGCACTGGGAGTGCCGGGGCCACCATTCGGCTGTACATCGA TAGCTATGAGAAGGACGTTGCCAAGATTAACCAGGACCCCCAGGTCATGTTGGCCCCCCTTATTTCCATT GCTCTGAAAGTGTCCCAGCTGCAGGAGAGGACGGGACGCACTGCACCCACTGTCATCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230308 representing NM_001172818
Red=Cloning site Green=Tags(s) MSDFEEWISGTYRKMEEGPLPLLTFATAPYHDQKPGTSGLRKKTYYFEEKPCYLENFIQSIFFSIDLKDR QGSSLVVGGDGRYFNKSAIETIVQMAAANGIGRLVIGQNGILSTPAVSCIIRKIKAIGGIILTASHNPGG PNGDFGIKFNISNGGPAPEAITDKIFQISKTIEEYAVCPDLKVDLGVLGKQQFDLENKFKPFTVEIVDSV EAYATMLRSIFDFSALKELLSGPNRLKIRIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHP DPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFA RSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSIL ATRKQSVEDILKDHWQKYGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKAD NFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISI ALKVSQLQERTGRTAPTVIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172818 |
| ORF Size | 1740 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172818.1, NP_001166289.1 |
| RefSeq ORF | 1743 bp |
| Locus ID | 5236 |
| UniProt ID | P36871 |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism |
| MW | 64.2 kDa |
| Gene Summary | The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230308L3 | Lenti-ORF clone of PGM1 (Myc-DDK-tagged)-Human phosphoglucomutase 1 (PGM1), transcript variant 2 |
CNY 6,460.00 |
|
| RC230308L4 | Lenti-ORF clone of PGM1 (mGFP-tagged)-Human phosphoglucomutase 1 (PGM1), transcript variant 2 |
CNY 6,460.00 |
|
| RG230308 | PGM1 (tGFP-tagged) - Human phosphoglucomutase 1 (PGM1), transcript variant 2 |
CNY 5,040.00 |
|
| SC328946 | PGM1 (untagged)-Human phosphoglucomutase 1 (PGM1) transcript variant 2 |
CNY 9,310.00 |
