CTAGE5 (MIA2) (NM_001247989) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234913 representing NM_001247989 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCCCGGGGTTACCCCTCAACCGTATTTGGGGCTGCTCCTGGAGGAGCTACGCAGGGTTGTGG CAGCACTGCCTGAAGGTATGAGACCAGATTCTAATCTTTATGGTTTTCCATGGGAATTGGTGATATGTGC AGCTGTTGTTGGATTTTTTGCTGTTCTCTTTTTTTTGTGGAGAAGTTTTAGATCGGTTAGGAGTCGGCTT TATGTGGGACGAGAGAAAAAGCTTGCTCTAATGCTTTCTGGACTAATTGAAGAAAAAAGTAAACTACTTG AAAAATTTAGCCTTGTTCAAAAAGAGTATGAAGGCTATGAAGTAGAGTCATCTTTAAAGGATGCCAGCTT TGAGAAGGAGGCAACAGAAGCACAAAGTTTGGAGGTAGAAAATCAAATGGCAACCTGTGAAAAGCTGAAC AGGTCCAATTCTGAACTTGAGGATGAAATACTCTGTCTAGAAAAAGAGTTAAAAGAAGAGAAATCCAAAC ATTCTGAACAAGATGAATTGATGGCGGATATTTCAAAAAGGATACAGTCTCTAGAAGATGAGTCAAAATC CCTCAAATCACAAGTAGCTGAAGCCAAAATGACCTTCAAGATATTTCAAATGAATGAAGAACGACTGAAG ATAGCAATAAAAGATGCTTTGAATGAAAATTCTCAACTTCAGGAAAGCCAGAAACAGCTTTTGCAAGAAG CTGAAGTATGGAAAGAACAAGTGAGTGAACTTAATAAACAGAAAGTAACATTTGAAGACTCCAAAGTACA TGCAGAACAAGTTCTAAATGATAAAGAAAGTCACATCAAGACTCTGACTGAACGCTTGTTAAAGATGAAA GATTGGGCTGCTATGCTTGGAGAAGACATAACGGATGATGATAACTTGGAATTAGAAATGAACAGTGAAT CGGAAAATGGTGCTTACTTAGATAATCCTCCAAAAGGAGCTTTGAAGAAACTGATTCATGCTGCTAAGTT AAATGCTTCTTTAAAAACCTTAGAAGGAGAAAGAAACCAAATTTATATTCAGTTGTCTGAAGTTGATAAA ACAAAGGAAGAGCTTACAGAGCATATTAAAAATCTTCAGACTGAACAAGCATCTTTGCAGTCAGAAAACA CACATTTTGAAAATGAGAATCAGAAGCTTCAACAGAAACTTAAAGTAATGACTGAATTATATCAAGAAAA TGAAATGAAACTCCACAGGAAATTAACAGTAGAGGAAAATTATCGGTTAGAGAAAGAAGAGAAACTTTCT AAAGTAGATGAAAAGATCAGCCATGCCACTGAAGAGCTGGAGACCTATAGAAAGCGAGCCAAAGATCTTG AAGAAGAATTGGAGAGAACTATTCATTCTTATCAAGGGCAGATTATTTCCCATGAGAAAAAAGCACATGA TAATTGGTTGGCAGCTCGGAATGCTGAAAGAAACCTCAATGATTTAAGGAAAGAAAATGCTCACAACAGA CAAAAATTAACTGAAACAGAGCTTAAATTTGAACTTTTAGAAAAAGATCCTTATGCACTCGATGTTCCAA ATACAGCATTTGGCAGAGAGCATTCCCCATATGGTCCCTCACCATTGGGTTGGCCTTCATCTGAAACAAG AGCTTTTCTCTCTCCTCCAACTTTGTTGGAGGGTCCACTCAGACTCTCACCTTTGCTTCCAGGGGGAGGA GGAAGAGGCTCACGAGGCCCAGGGAATCCTCTGGACCATCAGATTACCAATGAAAGAGGAGAATCAAGCT GTGATAGGTTAACCGATCCTCATAGGGCTCCCTCTGACACTGGGTCTCTGTCACCTCCATGGGACCAGGA CCGTAGGATGATGTTTCCTCCGCCAGGACAATCATATCCTGATTCAGCCCTTCCTCCACAAAGGCAAGAC AGATTTTGTTCTAATTCTGGTAGACTGTCTGGACCAGCAGAACTCAGAAGTTTTAATATGCCTTCTTTGG ATAAAATGGATGGGTCAATGCCTTCAGAAATGGAATCCAGTAGAAATGATACCAAAGATGATCTTGGTAA TTTAAATGTGCCTGATTCATCTCTCCCTGCTGAAAATGAAGCCACTGGCCCTGGCTTTGTTCCTCCACCT CTTGCTCCAATCAGAGGTCCATTGTTTCCAGTGGATGCAAGAGGCCCATTCTTGAGAAGAGGACCTCCTT TCCCCCCACCTCCTCCAGGAGCCATGTTTGGAGCTTCTCGAGATTATTTTCCACCAGGGGATTTCCCAGG TCCACCACCTGCTCCATTTGCAATGAGAAATGTCTATCCACCGAGGGGTTTTCCTCCTTACCTTCCCCCA AGACCTGGATTTTTCCCCCCACCCCCACATTCTGAAGGTAGAAGTGAGTTCCCCTCAGGTTTGATTCCAC CTTCAAATGAGCCTGCTACTGAACATCCAGAACCACAGCAAGAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234913 representing NM_001247989 Red=Cloning site Green=Tags(s) MEEPGVTPQPYLGLLLEELRRVVAALPEGMRPDSNLYGFPWELVICAAVVGFFAVLFFLWRSFRSVRSRL YVGREKKLALMLSGLIEEKSKLLEKFSLVQKEYEGYEVESSLKDASFEKEATEAQSLEVENQMATCEKLN RSNSELEDEILCLEKELKEEKSKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKMTFKIFQMNEERLK IAIKDALNENSQLQESQKQLLQEAEVWKEQVSELNKQKVTFEDSKVHAEQVLNDKESHIKTLTERLLKMK DWAAMLGEDITDDDNLELEMNSESENGAYLDNPPKGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDK TKEELTEHIKNLQTEQASLQSENTHFENENQKLQQKLKVMTELYQENEMKLHRKLTVEENYRLEKEEKLS KVDEKISHATEELETYRKRAKDLEEELERTIHSYQGQIISHEKKAHDNWLAARNAERNLNDLRKENAHNR QKLTETELKFELLEKDPYALDVPNTAFGREHSPYGPSPLGWPSSETRAFLSPPTLLEGPLRLSPLLPGGG GRGSRGPGNPLDHQITNERGESSCDRLTDPHRAPSDTGSLSPPWDQDRRMMFPPPGQSYPDSALPPQRQD RFCSNSGRLSGPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDDLGNLNVPDSSLPAENEATGPGFVPPP LAPIRGPLFPVDARGPFLRRGPPFPPPPPGAMFGASRDYFPPGDFPGPPPAPFAMRNVYPPRGFPPYLPP RPGFFPPPPHSEGRSEFPSGLIPPSNEPATEHPEPQQET TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001247989
ORF Size	2427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001247989.1, NP_001234918.1
RefSeq Size	3695 bp
RefSeq ORF	2430 bp
Locus ID	4253
UniProt ID	Q96PC5
Protein Families	Transmembrane
MW	92 kDa
Gene Summary	This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]

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