PRMT1 (NM_001207042) Human Tagged ORF Clone
CAT#: RC237187
- TrueORF®
PRMT1 (myc-DDK-tagged) - Human protein arginine methyltransferase 1 (PRMT1), transcript variant 4
ORF Plasmid: tGFP
"NM_001207042" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ANM1; HCP1; HRMT1L2; IR1B4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237187 representing NM_001207042
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCCGAGGCCGCGAACTGCATCATGGAGGTGTCCTGTGGCCAGGCGGAAAGCAGTGAGAAGC CCAACGCTGAGGACATGACATCCAAAGATTACTACTTTGACTCCTACGCACACTTTGGCATCCACGAGGA GATGCTGAAGGACGAGGTGCGCACCCTCACTTACCGCAACTCCATGTTTCATAACCGGCACCTCTTCAAG GACAAGGTGGTGCTGGACGTCGGCTCGGGCACCGGCATCCTCTGCATGTTTGCTGCCAAGGCCGGGGCCC GCAAGGTCATCGGGATCGAGTGTTCCAGTATCTCTGATTATGCGGTGAAGATCGTCAAAGCCAACAAGTT AGACCACGTGGTGACCATCATCAAGGGGAAGGTGGAGGAGGTGGAGCTCCCAGTGGAGAAGGTGGACATC ATCATCAGCGAGTGGATGGGCTACTGCCTCTTCTACGAGTCCATGCTCAACACCGTGCTCTATGCCCGGG ACAAGTGGCTGGAGGTGGACATCTATACCGTCAAGGTGGAAGACCTGACCTTCACCTCCCCGTTCTGCCT GCAAGTGAAGCGGAATGACTACGTGCACGCCCTGGTGGCCTACTTCAACATCGAGTTCACACGCTGCCAC AAGAGGACCGGCTTCTCCACCAGCCCCGAGTCCCCGTACACGCACTGGAAGCAGACGGTGTTCTACATGG AGGACTACCTGACCGTGAAGACGGGCGAGGAGATCTTCGGCACCATCGGCATGCGGCCCAACGCCAAGAA CAACCGGGACCTGGACTTCACCATCGACCTGGACTTCAAGGGCCAGCTGTGCGAGCTGTCCTGCTCCACC GACTACCGGATGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237187 representing NM_001207042
Red=Cloning site Green=Tags(s) MAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFK DKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDI IISEWMGYCLFYESMLNTVLYARDKWLEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCH KRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCST DYRMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001207042 |
ORF Size | 855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001207042.3 |
RefSeq Size | 1192 bp |
RefSeq ORF | 858 bp |
Locus ID | 3276 |
UniProt ID | Q99873 |
MW | 33.1 kDa |
Gene Summary | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011] |
Documents
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