RUSC1 (NM_001278229) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NESCA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237464 representing NM_001278229 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAGCCCAGAGTGGGACTGGTCAGCTGCAGGAGCAGAAGAAAGGTCTTCTGATAGCCGTCAGCG TCTCCGTTGATAAAATCATCTCGCATTTCGGGGCCGCCCGGAACTTGGTGCAGAAGGCCCAGTTGGGTGA TAGCCGGCTGAGCCCGGATGTGGGGCACCTGGTGCTGACCACCCTCTGCCCGGCCCTCCACGCCCTGGTG GCGGACGGGCTGAAGCCTTTCCGGAAGGACCTCATCACCGGGCAGCGCAGGAGCAGCCCCTGGAGCGTGG TGGAGGCGTCGGTGAAGCCAGGCTCCAGCACCCGCTCCCTTGGAACCCTGTATAGCCAGGTCAGCCGTCT AGCCCCGCTGAGCAGCAGCCGTAGCCGCTTCCATGCCTTTATCCTGGGCCTCCTCAACACCAAGCAGTTG GAGCTGTGGTTTTCCAGTCTCCAGGAAGATGCAGGGAGCTGGTGGGAGCAGTTGACCCAGGCCTCCCGGG TCTATGCCTCTGGGGGCACTGAGGGCTTTCCTCTTTCCCGATGGGCACCGGGGCGTCATGGGACTGCAGC TGAAGAAGGTGCACAGGAGAGACCCCTGCCCACAGATGAGATGGCACCAGGCAGGGGCCTCTGGTTGGGA AGACTATTTGGAGTGCCTGGGGGCCCCGCAGAAAATGAGAATGGAGCCCTAAAGTCCAGGAGACCATCTA GCTGGCTGCCCCCGACAGTGAGTGTGTTGGCTCTTGTGAAGCGGGGGGCACCTCCCGAGATGCCTTCTCC TCAGGAGCTTGAGGCCTCAGCACCCAGGATGGTGCAAACCCATAGGGCAGTGCGGGCTCTCTGTGATCAC ACTGCTGCAAGACCTGACCAGTTGAGCTTCCGGCGTGGGGAAGTGCTGCGTGTCATCACCACAGTGGATG AGGACTGGCTCCGCTGTGGGCGGGATGGCATGGAGGGTCTGGTGCCTGTGGGGTATACCTCCCTTGTTCT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237464 representing NM_001278229 Red=Cloning site Green=Tags(s) MAEAQSGTGQLQEQKKGLLIAVSVSVDKIISHFGAARNLVQKAQLGDSRLSPDVGHLVLTTLCPALHALV ADGLKPFRKDLITGQRRSSPWSVVEASVKPGSSTRSLGTLYSQVSRLAPLSSSRSRFHAFILGLLNTKQL ELWFSSLQEDAGSWWEQLTQASRVYASGGTEGFPLSRWAPGRHGTAAEEGAQERPLPTDEMAPGRGLWLG RLFGVPGGPAENENGALKSRRPSSWLPPTVSVLALVKRGAPPEMPSPQELEASAPRMVQTHRAVRALCDH TAARPDQLSFRRGEVLRVITTVDEDWLRCGRDGMEGLVPVGYTSLVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278229
ORF Size	981 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278229.1, NP_001265158.1
RefSeq Size	2250 bp
RefSeq ORF	984 bp
Locus ID	23623
UniProt ID	Q9BVN2
MW	35.8 kDa
Gene Summary	Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF-dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway.[UniProtKB/Swiss-Prot Function]

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