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Dehydrodolichyl Diphosphate Synthase (DHDDS) (NM_024887) Human Tagged ORF Clone
CAT＃: RG201430
- TrueORF®
DHDDS (tGFP-tagged) - Human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1
ORF Plasmid: DDK
"NM_024887" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CIT; CPT; DEDSM; DS; hCIT; HDS; RP59 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201430 representing NM_024887
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATGGATCAAGGAAGGAGAGCTGTCACTTTGGGAGCGGTTCTGTGCCAACATCATAAAGGCAGGCC CAATGCCGAAACACATTGCATTCATAATGGACGGGAACCGTCGCTATGCCAAGAAGTGCCAGGTGGAGCG GCAGGAAGGCCACTCACAGGGCTTCAACAAGCTAGCTGAGACTCTGCGGTGGTGTTTGAACCTGGGCATC CTAGAGGTGACAGTCTACGCATTCAGCATTGAGAACTTCAAACGCTCCAAGAGTGAGGTAGACGGGCTTA TGGATCTGGCCCGGCAGAAGTTCAGCCGCTTGATGGAAGAAAAGGAGAAACTGCAGAAGCATGGGGTGTG TATCCGGGTCCTGGGCGATCTGCACTTGTTGCCCTTGGATCTCCAGGAGCTGATTGCACAAGCTGTACAG GCCACGAAGAACTACAACAAGTGTTTCCTGAATGTCTGTTTTGCATACACATCCCGTCATGAGATCAGCA ATGCTGTGAGAGAGATGGCCTGGGGGGTGGAGCAAGGCCTGTTGGATCCCAGTGATATCTCTGAGTCTCT GCTTGATAAGTGCCTCTATACCAACCGCTCTCCTCATCCTGACATCTTGATACGGACTTCTGGAGAAGTG CGGCTGAGTGACTTCTTGCTATGGCAGACCTCTCACTCCTGCCTGGTGTTCCAACCCGTTCTGTGGCCAG AGTATACATTTTGGAACCTCTTCGAGGCCATCCTGCAGTTCCAGATGAACCATAGCATGCTTCAGAAGGC CCGAGACATGTATGCAGAGGAGCGGAAGAGGCAGCAGCTGGAGAGGGACCAGGCTACAGTGACAGAGCAG CTGCTGCGAGAGGGGCTCCAAGCCAGTGGGGACGCCCAGCTCCGAAGGACACGCTTGCACAAACTCTCGG CCAGACGGGAAGAGCGAGTCCAAGGCTTCCTGCAGGCCTTGGAACTCAAGCGAGCTGACTGGCTGGCCCG TCTGGGCACTGCATCAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201430 representing NM_024887
Red=Cloning site Green=Tags(s) MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGI LEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQ ATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEV RLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQATVTEQ LLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024887 |
| ORF Size | 999 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024887.2, NP_079163.2 |
| RefSeq Size | 3315 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 79947 |
| UniProt ID | Q86SQ9 |
| Domains | UPP_synthetase |
| Protein Pathways | Terpenoid backbone biosynthesis |
| Gene Summary | The protein encoded by this gene catalyzes cis-prenyl chain elongation to produce the polyprenyl backbone of dolichol, a glycosyl carrier lipid required for the biosynthesis of several classes of glycoproteins. Mutations in this gene are associated with retinitis pigmentosa type 59. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201430 | DHDDS (Myc-DDK-tagged)-Human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1 |
CNY 2,400.00 |
|
| RC201430L3 | Lenti ORF clone of Human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201430L4 | Lenti ORF clone of Human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC321506 | DHDDS (untagged)-Human dehydrodolichyl diphosphate synthase (DHDDS), transcript variant 1 |
CNY 2,950.00 |
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