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GNAI3 (NM_006496) Human Tagged ORF Clone
CAT＃: RG206850
- TrueORF®
GNAI3 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3)
ORF Plasmid: DDK
"NM_006496" in other vectors (7)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 87U6; ARCND1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206850 representing NM_006496
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACGTTGAGCGCCGAAGACAAGGCGGCAGTGGAGCGAAGCAAGATGATCGACCGCAACTTAC GGGAGGACGGGGAAAAAGCGGCCAAAGAAGTGAAGCTGCTGCTACTCGGTGCTGGAGAATCTGGTAAAAG CACCATTGTGAAACAGATGAAAATCATTCATGAGGATGGCTATTCAGAGGATGAATGTAAACAATATAAA GTAGTTGTCTACAGCAATACTATACAGTCCATCATTGCAATCATAAGAGCCATGGGACGGCTAAAGATTG ACTTTGGGGAAGCTGCCAGGGCAGATGATGCCCGGCAATTATTTGTTTTAGCTGGCAGTGCTGAAGAAGG AGTCATGACTCCAGAACTAGCAGGAGTGATTAAACGGTTATGGCGAGATGGTGGGGTACAAGCTTGCTTC AGCAGATCCAGGGAATATCAGCTCAATGATTCTGCTTCATATTATCTAAATGATCTGGATAGAATATCCC AGTCTAACTACATTCCAACTCAGCAAGATGTTCTTCGGACGAGAGTGAAGACCACAGGCATTGTAGAAAC ACATTTCACCTTCAAAGACCTATACTTCAAGATGTTTGATGTAGGTGGCCAAAGATCAGAACGAAAAAAG TGGATTCACTGTTTTGAGGGAGTGACAGCAATTATCTTCTGTGTGGCCCTCAGTGATTATGACCTTGTTC TGGCTGAGGACGAGGAGATGAACCGAATGCATGAAAGCATGAAACTGTTTGACAGCATTTGTAATAACAA ATGGTTTACAGAAACTTCAATCATTCTCTTCCTTAACAAGAAAGACCTTTTTGAGGAAAAAATAAAGAGG AGTCCGTTAACTATCTGTTATCCAGAATACACAGGTTCCAATACATATGAAGAGGCAGCTGCCTATATTC AATGCCAGTTTGAAGATCTGAACAGAAGAAAAGATACCAAGGAGATCTATACTCACTTCACCTGTGCCAC AGACACGAAGAATGTGCAGTTTGTTTTTGATGCTGTTACAGATGTCATCATTAAAAACAACTTAAAGGAA TGTGGACTTTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206850 representing NM_006496
Red=Cloning site Green=Tags(s) MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYK VVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACF SRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKK WIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKR SPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKE CGLY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006496 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006496.4 |
| RefSeq Size | 3143 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 2773 |
| UniProt ID | P08754 |
| Domains | G-alpha |
| Protein Families | Druggable Genome |
| Protein Pathways | Axon guidance, Chemokine signaling pathway, Gap junction, Leukocyte transendothelial migration, Long-term depression, Melanogenesis, Progesterone-mediated oocyte maturation, Tight junction |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206850 | GNAI3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3) |
CNY 3,656.00 |
|
| RC206850L1 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206850L2 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), mGFP tagged |
CNY 5,890.00 |
|
| RC206850L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206850L4 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), mGFP tagged |
CNY 5,890.00 |
|
| SC116066 | GNAI3 (untagged)-Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3) |
CNY 2,950.00 |
|
| SC320804 | GNAI3 (untagged)-Human guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3) |
CNY 2,950.00 |
