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RBPJK (RBPJ) (NM_015874) Human Tagged ORF Clone
CAT＃: RG215872
- TrueORF®
RBPJ (tGFP-tagged) - Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2
ORF Plasmid: DDK
"NM_015874" in other vectors (6)
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CNY 4,750.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AOS3; CBF-1; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBP-JK; RBP-J kappa; RBPJK; RBPSUH; SUH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215872 representing NM_015874
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTGTTGTGACAGGGAAATTTGGTGAGCGGCCTCCACCTAAACGACTTACTAGGGAAGCTATGC GAAATTATTTAAAAGAGCGAGGGGATCAAACAGTACTTATTCTTCATGCAAAAGTTGCACAGAAGTCATA TGGAAATGAAAAAAGGTTTTTTTGCCCACCTCCTTGTGTATATCTTATGGGCAGTGGATGGAAGAAAAAA AAAGAACAAATGGAACGCGATGGTTGTTCTGAACAAGAGTCTCAACCGTGTGCATTTATTGGGATAGGAA ATAGTGACCAAGAAATGCAGCAGCTAAACTTGGAAGGAAAGAACTATTGCACAGCCAAAACATTGTATAT ATCTGACTCAGACAAGCGAAAGCACTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGTGATGACATT GGTGTGTTCCTCAGCAAGCGGATAAAAGTCATCTCCAAACCTTCCAAAAAGAAGCAGTCATTGAAAAATG CTGACTTATGCATTGCCTCAGGAACAAAGGTGGCTCTGTTTAATCGACTACGATCCCAGACAGTTAGTAC CAGATACTTGCATGTAGAAGGAGGTAATTTTCATGCCAGTTCACAGCAGTGGGGAGCCTTTTTTATTCAT CTCTTGGATGATGATGAATCAGAAGGAGAAGAATTCACAGTCCGAGATGGCTACATCCATTATGGACAAA CAGTCAAACTTGTGTGCTCAGTTACTGGCATGGCACTCCCAAGATTGATAATTAGGAAAGTTGATAAGCA GACCGCATTATTGGATGCAGATGATCCTGTGTCACAACTCCATAAATGTGCATTTTACCTTAAGGATACA GAAAGAATGTATTTGTGCCTTTCTCAAGAAAGAATAATTCAATTTCAGGCCACTCCATGTCCAAAAGAAC CAAATAAAGAGATGATAAATGATGGCGCTTCCTGGACAATCATTAGCACAGATAAGGCAGAGTATACATT TTATGAGGGAATGGGCCCTGTCCTTGCCCCAGTCACTCCTGTGCCTGTGGTAGAGAGCCTTCAGTTGAAT GGCGGTGGGGACGTAGCAATGCTTGAACTTACAGGACAGAATTTCACTCCAAATTTACGAGTGTGGTTTG GGGATGTAGAAGCTGAAACTATGTACAGGTGTGGAGAGAGTATGCTCTGTGTCGTCCCAGACATTTCTGC ATTCCGAGAAGGTTGGAGATGGGTCCGGCAACCAGTCCAGGTTCCAGTAACTTTGGTCCGAAATGATGGA ATCATTTATTCCACCAGCCTTACCTTTACCTACACACCAGAACCAGGGCCGCGGCCACATTGCAGTGCAG CAGGAGCAATCCTTCGAGCCAATTCAAGCCAGGTGCCCCCTAACGAATCAAACACAAACAGCGAGGGAAG TTACACAAACGCCAGCACAAATTCAACCAGTGTCACATCATCTACAGCCACAGTGGTATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215872 representing NM_015874
Red=Cloning site Green=Tags(s) MAPVVTGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKK KEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDI GVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIH LLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDT ERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLN GGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDG IIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015874 |
| ORF Size | 1461 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015874.5 |
| RefSeq Size | 5825 bp |
| RefSeq ORF | 1464 bp |
| Locus ID | 3516 |
| UniProt ID | Q06330 |
| Protein Families | Transcription Factors |
| Protein Pathways | Notch signaling pathway |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215872 | RBPJ (Myc-DDK-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 5,488.00 |
|
| RC215872L1 | Lenti-ORF clone of RBPJ (Myc-DDK-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 7,888.00 |
|
| RC215872L2 | Lenti-ORF clone of RBPJ (mGFP-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 6,180.00 |
|
| RC215872L3 | Lenti-ORF clone of RBPJ (Myc-DDK-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 6,180.00 |
|
| RC215872L4 | Lenti-ORF clone of RBPJ (mGFP-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 6,180.00 |
|
| SC108442 | RBPJ (untagged)-Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 2 |
CNY 5,488.00 |
