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Histidase (HAL) (NM_001258333) Human Tagged ORF Clone
CAT＃: RG232751
- TrueORF®
HAL (tGFP-tagged) - Homo sapiens histidine ammonia-lyase (HAL), transcript variant 2
ORF Plasmid: DDK
"NM_001258333" in other vectors (2)
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CNY 4,940.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HIS; HSTD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232751 representing NM_001258333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTTGGCTTTAAGGATCAATGTCTTAGCCAAAGGATACAGTGGCATTTCCCTGGAGACCCTCAAAC AAGTCATAGAAATGTTTAATGCCTCCTGCCTGCCCTATGTCCCAGAGAAAGGAACCGTTGGTGCCAGTGG AGACCTTGCCCCACTCTCTCATCTTGCTCTTGGGCTAGTTGGAGAAGGGAAGATGTGGTCTCCGAAGAGT GGCTGGGCTGATGCTAAATACGTGCTAGAAGCCCATGGATTGAAACCAGTTATTTTAAAACCAAAAGAGG GCCTGGCACTCATCAATGGGACGCAGATGATCACATCCCTGGGCTGTGAAGCTGTAGAGCGAGCCAGTGC TATTGCACGGCAGGCTGACATTGTGGCAGCCCTGACCCTTGAGGTGCTGAAGGGCACCACCAAAGCCTTT GACACTGACATTCATGCTCTTCGACCTCACCGTGGGCAAATTGAAGTTGCTTTTCGGTTTCGGTCACTCT TGGACTCAGATCACCACCCATCAGAAATAGCAGAGAGTCACAGGTTCTGTGATCGCGTCCAGGATGCATA CACCTTGCGCTGCTGTCCACAGGTCCATGGTGTGGTGAATGATACAATAGCATTTGTGAAGAACATCATT ACCACAGAACTGAACAGCGCAACAGATAATCCTATGGTCTTTGCCAATAGGGGAGAGACAGTTTCTGGAG GAAACTTCCATGGTGAATACCCAGCCAAAGCCCTAGACTACTTGGCCATTGGCATCCATGAACTTGCTGC AATCAGTGAGAGAAGAATCGAGCGGCTCTGCAATCCCTCCCTCAGTGAGCTGCCTGCCTTCCTGGTGGCT GAAGGTGGTCTGAACTCTGGGTTCATGATAGCTCACTGCACGGCAGCAGCCCTTGTTTCTGAGAACAAGG CTCTGTGCCATCCCTCGTCTGTTGACTCCCTCTCCACCAGCGCAGCCACGGAGGACCACGTCTCCATGGG AGGATGGGCAGCAAGGAAAGCCCTCAGGGTCATCGAGCATGTGGAGCAAGTGCTGGCCATCGAGCTCCTT GCAGCCTGCCAGGGCATAGAGTTTCTACGTCCCCTGAAAACAACCACTCCGCTGGAGAAGGTCTATGACC TGGTGCGCTCTGTTGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCGGACATCGAGGCAGCCCACAG GCTGCTCCTGGAGCAGAAGGTTTGGGAAGTAGCTGCTCCATACATTGAAAAATACAGAATGGAGCATATT CCAGAATCAAGACCTCTTTCTCCAACAGCCTTTTCACTGCAATTTCTGCACAAGAAATCCACCAAAATCC CGGAGTCTGAGGACCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232751 representing NM_001258333
Red=Cloning site Green=Tags(s) MLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPLSHLALGLVGEGKMWSPKS GWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAF DTDIHALRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKNII TTELNSATDNPMVFANRGETVSGGNFHGEYPAKALDYLAIGIHELAAISERRIERLCNPSLSELPAFLVA EGGLNSGFMIAHCTAAALVSENKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELL AACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHI PESRPLSPTAFSLQFLHKKSTKIPESEDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258333 |
| ORF Size | 1347 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258333.1, NP_001245262.1 |
| RefSeq Size | 3860 bp |
| RefSeq ORF | 1350 bp |
| Locus ID | 3034 |
| UniProt ID | P42357 |
| Protein Families | Druggable Genome |
| Protein Pathways | Histidine metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
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