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WASF2 (NM_001201404) Human Tagged ORF Clone
CAT＃: RG233631
- TrueORF®
WASF2 (tGFP-tagged) - Homo sapiens WAS protein family, member 2 (WASF2), transcript variant 2
ORF Plasmid: DDK
"NM_001201404" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dJ393P12.2; IMD2; SCAR2; WASF4; WAVE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233631 representing NM_001201404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTTAGTAACGAGGAACATCGAGCCAAGGCACCTGTGCCGTCAGACGTTGCCTAGCGTTAGAAGCG AGCTGGAATGCGTGACCAACATCACCCTGGCAAATGTCATCCGACAGCTGGGCAGCCTGAGTAAATATGC AGAGGACATTTTTGGAGAGCTCTTTACTCAGGCAAATACCTTTGCCTCTCGGGTAAGCTCCCTTGCTGAG AGGGTCGACCGACTACAGGTTAAAGTCACTCAGCTGGATCCCAAGGAAGAAGAAGTGTCACTGCAAGGAA TCAACACCCGAAAAGCCTTCAGAAGTTCCACCATTCAAGACCAGAAGCTTTTTGACAGAAACTCTCTCCC AGTGCCTGTCTTAGAAACATACAATACCTGTGATACTCCTCCCCCTCTCAACAATCTTACCCCTTACAGG GACGATGGAAAAGAGGCACTCAAATTCTACACAGACCCTTCATACTTCTTTGATCTTTGGAAGGAGAAGA TGCTGCAGGACACCAAGGATATCATGAAAGAGAAGAGAAAGCATAGGAAAGAAAAGAAAGATAATCCAAA TCGAGGGAATGTAAACCCACGTAAAATCAAGACACGTAAGGAAGAGTGGGAGAAAATGAAGATGGGGCAA GAATTTGTGGAGTCCAAAGAAAAGCTGGGGACTTCTGGGTATCCACCCACTTTGGTGTACCAGAATGGCA GCATTGGCTGTGTTGAAAACGTGGATGCAAGTAGCTATCCGCCACCACCACAGTCAGACTCTGCTTCTTC ACCTTCTCCTTCCTTCTCCGAGGACAACTTGCCTCCTCCACCAGCAGAATTCAGGTTTTCAGCTGCGCAG GGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233631 representing NM_001201404
Red=Cloning site Green=Tags(s) MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAE RVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYR DDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQ EFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFRFSAAQ G TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001201404 |
| ORF Size | 843 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001201404.3 |
| RefSeq Size | 5161 bp |
| RefSeq ORF | 846 bp |
| Locus ID | 10163 |
| UniProt ID | Q9Y6W5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
Documents
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