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Egf (NM_012842) Rat Tagged ORF Clone
CAT＃: RR201131
- TrueORF®
Egf (Myc-DDK-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug)
"NM_012842" in other vectors (3)
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CNY 12,624.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201131 representing NM_012842
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTCTCGCTCACCTTCCTGTCGGTGTTTTTAAAGATTACTGTACTCAGTGTCACAGCACAGCAGA CCAGGAACTGTCAGTCAGGTCCTCTCGAGAGAAGCGGGACTACCACGTATGCCGCCGCCGGTCCTCCCAG GTTCCTGATTTTCTTACAAGGAAACAGCATCTTTCGGATTAACACAGATGGAACAAATCACCAGCAATTG GTGGTGGATGCCGGCGTCTCAGTGGTCATGGATTTTCATTACAAGGAAGAGAGACTCTATTGGGTGGATT TAGAAAGACAACTTTTGCAAAGAGTTTTCTTTAATGGGTCAGGACAAGAGACAGTGTGCAAGGTGGATAA GAATGTGTCTGGGCTGGCCATAAACTGGATAGATGGGGAGATTCTCCGGACGGACCGATGGAAGGGAGTC ATCACAGTAACAGATATGAACGGGAACAATTCCCGTGTTCTTCTGAGTTCCTTAAAACGTCCTGCAAATA TATTAGTGGATCCAACAGAGAGGTTGATATTTTGGTCTTCAGTGGTGACTGGCAACCTTCACAGAGCAGA TCTCGGGGGTATGGATGTAAAAACACTGCTGGAGGCACCAGAGAGGATATCAGTGCTGATTCTGGATATC CTGGACAAAAGGCTCTTCTGGGCTCAGGACGGTAGAGAAGGAAGCCACGGTTACATTCACTCCTGTGACT ATAACGGTGGCTCCATCCATCATATCAGACATCAAGCACGGCACGATTTGCTTACTATGGCCATTTTCGG TGACAAGATCTTATACTCAGCACTGAAAGAGAAGGCGATTTGGATAGCCGACAAACACACTGGGAAGAAT GTGGTTCGAGTTAACCTCGATCCAGCCTCTGTGCCGCCAAGAGAACTGAGAGTCGTGCACCTACATGCAC AGCCCGGGACAGAGAACCGTGCTCAGGCCTCTGACTCCGAACGATGCAAACAGAGAAGAGGACAGTGTCT CTACAGTCTCTCTGAGCGAGACCCCAACTCAGACTCGTCGGCATGCGCTGAAGGCTATACGTTAAGCCGA GACCGGAAGTACTGCGAAGATGTCAATGAGTGTGCCTTGCAGAATCACGGCTGTACTCTTGGGTGTGAAA ACATCCCTGGATCCTATTACTGCACATGCCCTACAGGCTTTGTTCTGCTTCCTGATGGGAAACGATGTCA CGAACTTGTTGCCTGTCCAGGCAACAGATCAGAGTGTAGCCATGATTGCATCCTGACATCAGATGGTCCT CTGTGCATCTGTCCAGCAGGTTCAGTGCTCGGAAAAGATGGGAAGACATGCACTGGTTGTTCCTTCTCCG ATAATGGTGGATGCAGCCAGATCTGCCTTCCTCTCAGCCTAGCATCCTGGGAATGTGATTGCTTTCCTGG GTACGACCTACAATTGGACCGAAAGAGCTGTGCAGCTTCCATGGGACCGCAGCCATTTTTACTGTTTGCA AATTCCCAGGACATACGACACATGCATTTTGATGGAACAGACTACAAAACTCTGCTCAGCCGGCAGATGG GAATGGTTTTTGCCTTGGATTATGACCCCGTGGAAAGCAAGATATATTTTGCACAGACAGCCCTGAAGTG GATAGAGAGGGCTAATCTGGATGGCTCCCAGCGAGAAAGACGGATCACGGAAGGAGTAGACACGCCAGAA GGTCTTGCCGTGGACTGGATTGGCCGGAGAATCTACTGGACGGACAGTGGGAAGTCTGTCATTGAAGGGA GTGATTTGAGCGGGAAGCATCATCAAATAATCATCAAAGAGAGCATCTCAAGGCCACGAGGAATAGCTGT GCATCCAAAGGCCAGGAGACTATTCTGGACGGACACGGGGATGTCTCCGCGGATTGAAAGCTCTTCCCTT CAAGGTTCTGACCGGACGCTGATAGCCAGCTCTAATCTACTGGAACCCAGTGGAATCGCGATTGACTACT TAACAGACACTTTGTACTGGTGTGACACCAAGCTGTCTGTGATTGAAATGGCCGATCTAGATGGTTCCAA ACGCCGCAGACTTACCCAGAACGATGTAGGTCACCCATTCTCTCTAGCTGTGTTTGAGGATCACGTGTGG TTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGAGGACTGGTCAAAACAGGGTACGTCTCC GAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCACCCATTGGCAAAACCAGGTGCAGACCCCTG CTTACACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGCCTGGGCACGGCTCAGTGTCTGTGTCGG GAAGGATTCGTGAAGGCCCCAGATGGGAAAATGTGTCTCACTCGGAAGGATGATCAGATACTGGCCGGTG ACAATGCTGATCTTAGTAAAGAGGTGGCATCGTTGGACAACTCCCCTAAGGCTTATGTACCAGACGATGA TAGGACAGAGTCCTCCACACTAGTGGCTGAGATCATGGTGTCAGGGCTGAACTATGAAGATGACTGCGGC CCTGGTGGGTGTGGCAGCCATGCCCACTGTATTTCAGAGGGAGAGGCAGCTGTGTGTCAGTGTTTGAAAG GATTTGCTGGCGATGGAAACCTGTGTTCTGATATAGACGAATGTGAGCTGGGTAGCTCAGACTGTCCTCC CACCTCGTCCAGGTGCATCAACACCGAAGGTGGCTATGTCTGCCAATGCTCAGAAGGCTACGAGGGAGAT GGGATCTACTGTCTCGACGTTGATGAGTGCCAGCAGGGGTCGCACGGCTGCAGCGAGAATGCCACCTGCA CCAACACGGAGGGAGGCTACAACTGCACCTGTGCAGGCTGCCCATCAGCACCTGGACTGCCTTGCCCTGA CTCTACCTCACCCTCTCTCCTTGGAAAAGATGGCTGCCACTGGGTCCGAAACAGTAACACAGGATGCCCG CCGTCGTACGATGGGTACTGCCTCAATGGTGGCGTGTGCATGTATGTTGAATCCGTGGACCGCTACGTGT GCAACTGTGTCATTGGCTATATTGGAGAACGATGTCAGCACCGAGACTTACGTTGGTGGAAGCTGCGCCA TGCTGACTACGGGCAGAGGCACGACATCACTGTGGTGTCTGTCTGTGTGGTGGCGCTGGCCCTGCTGCTC CTCTTAGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGCTATCAGAGAGCTCAAAGAAGCCTT CCGAAGAGTCAAGCAGCAACGTGAGCAGTAACGGGCCTGACAGCAGCGGGGCTGGGGTGTCTTCTGGTCC CCAACCTTGGTTTGTGGTCCTAGAGGAACACCAACAGCCCAAGAATGGGCGTCTGCCTGCCGCTGGCACG AACGGCGCAGTAGTAGAGGCTGGCCTGTCTTCCTCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201131 representing NM_012842
Red=Cloning site Green=Tags(s) MLFSLTFLSVFLKITVLSVTAQQTRNCQSGPLERSGTTTYAAAGPPRFLIFLQGNSIFRINTDGTNHQQL VVDAGVSVVMDFHYKEERLYWVDLERQLLQRVFFNGSGQETVCKVDKNVSGLAINWIDGEILRTDRWKGV ITVTDMNGNNSRVLLSSLKRPANILVDPTERLIFWSSVVTGNLHRADLGGMDVKTLLEAPERISVLILDI LDKRLFWAQDGREGSHGYIHSCDYNGGSIHHIRHQARHDLLTMAIFGDKILYSALKEKAIWIADKHTGKN VVRVNLDPASVPPRELRVVHLHAQPGTENRAQASDSERCKQRRGQCLYSLSERDPNSDSSACAEGYTLSR DRKYCEDVNECALQNHGCTLGCENIPGSYYCTCPTGFVLLPDGKRCHELVACPGNRSECSHDCILTSDGP LCICPAGSVLGKDGKTCTGCSFSDNGGCSQICLPLSLASWECDCFPGYDLQLDRKSCAASMGPQPFLLFA NSQDIRHMHFDGTDYKTLLSRQMGMVFALDYDPVESKIYFAQTALKWIERANLDGSQRERRITEGVDTPE GLAVDWIGRRIYWTDSGKSVIEGSDLSGKHHQIIIKESISRPRGIAVHPKARRLFWTDTGMSPRIESSSL QGSDRTLIASSNLLEPSGIAIDYLTDTLYWCDTKLSVIEMADLDGSKRRRLTQNDVGHPFSLAVFEDHVW FSDWAIPSVIRVNKRTGQNRVRLRGSMLKPSSLVVVHPLAKPGADPCLHRNGGCEHICQESLGTAQCLCR EGFVKAPDGKMCLTRKDDQILAGDNADLSKEVASLDNSPKAYVPDDDRTESSTLVAEIMVSGLNYEDDCG PGGCGSHAHCISEGEAAVCQCLKGFAGDGNLCSDIDECELGSSDCPPTSSRCINTEGGYVCQCSEGYEGD GIYCLDVDECQQGSHGCSENATCTNTEGGYNCTCAGCPSAPGLPCPDSTSPSLLGKDGCHWVRNSNTGCP PSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLRHADYGQRHDITVVSVCVVALALLL LLGMWGTYYYRTRKQLSESSKKPSEESSSNVSSNGPDSSGAGVSSGPQPWFVVLEEHQQPKNGRLPAAGT NGAVVEAGLSSSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012842 |
| ORF Size | 3399 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012842.1, NP_036974.1 |
| RefSeq Size | 4801 bp |
| RefSeq ORF | 3402 bp |
| Locus ID | 25313 |
| UniProt ID | P07522 |
| MW | 124.1 kDa |
| Gene Summary | binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201131 | Egf (untagged ORF) - Rat epidermal growth factor (Egf), (10 ug) |
CNY 11,840.00 |
|
| RR201131L3 | Lenti ORF clone of Egf (Myc-DDK-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug) |
CNY 19,760.00 |
|
| RR201131L4 | Lenti ORF clone of Egf (mGFP-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug) |
CNY 21,760.00 |
