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Pcca (NM_019330) Rat Tagged ORF Clone
CAT＃: RR205851
- TrueORF®
Pcca (Myc-DDK-tagged ORF) - Rat propionyl-coenzyme A carboxylase, alpha polypeptide (Pcca), (10 ug)
"NM_019330" in other vectors (3)
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CNY 9,310.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205851 representing NM_019330
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCTGTGGGTCAGGACAGTGGCACTATTGGCGGCCAGGCGGCATTGGCGGCGGTCCTCCCAGC AACTGCTGTGGACGCTGAAGCGTGCTCCAGTCTATTCACAGCAATGCCTAGTGGTGTCCCGAAGTCTCAG TTCTGTGGAATATGAGCCTAAAGAAAAAACTTTTGATAAAATTCTTATTGCTAACAGAGGAGAAATTGCT TGTAGGGTCATTAAGACTTGCAGGAAGATGGGCATCAGGACAGTTGCCATCCACAGTGATGTTGATGCCA GCTCGGTTCATGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCT CAACATGGATGCCATCATGGAGGCCATTAAGAAAACCGGGGCCCAAGCTGTACACCCAGGTTATGGATTC CTGTCAGAAAACAAAGAGTTTGCAAAGTGTTTGGCAGCAGAAGATGTCACTTTCATTGGACCTGATACTC ATGCTATTCAAGCCATGGGTGACAAGATAGAAAGCAAACTGTTAGCCAAGAGAGCAAAGGTCAACACAAT CCCTGGTTTTGATGGGGTACTAAAGGATGCAGATGAAGCCGTCAGAATTGCAAGGGAAATTGGCTACCCT GTGATGATCAAGGCCTCAGCAGGCGGTGGTGGGAAAGGCATGCGCATCCCGTGGGATGACGAAGAGACCA GGGATGGCTTTAGATTTTCATCCCAGGAAGCTGCTTCTAGTTTTGGTGATGATAGACTACTAATAGAAAA ATTTATCGATAACCCTCGTCATATAGAAATCCAGGTTTTAGGGGATAAACATGGCAATGCTCTGTGGCTC AATGAGAGGGAGTGCTCGATCCAGAGAAGAAACCAGAAGGTGGTAGAGGAGGCGCCGAGCATTTTTCTGG ATCCTGAGACGCGCCGAGCCATGGGAGAGCAGGCCGTGGCTTGGCCAAAAGCGGTGAAGTATTCTTCTGC TGGAACTGTGGAATTTCTTGTGGACTCCCAGAAGAATTTTTACTTCTTGGAGATGAATACAAGACTCCAG GTTGAACATCCTGTCACAGAGTGCATTACTGGCCTGGACTTAGTCCAAGAAATGATCCTGGTTGCTAAGG GTTACCCACTCAGGCACAAGCAAGAGGATATTCCCATCAGTGGCTGGGCAGTTGAATGTCGGGTTTATGC TGAGGACCCCTACAAGTCTTTCGGTTTACCGTCTATTGGGAGACTGTCTCAGTACCAAGAGCCTATACAT CTACCTGGTGTCCGAGTTGACAGTGGCATCCAACCAGGAAGTGACATCAGCATCTATTATGACCCTATGA TTTCAAAACTAGTCACATATGGATCTGACAGAGCAGAAGCCCTGAAGAGGATGGAAGATGCATTGGACAG TTATGTTATCCGGGGTGTTACACACAACATCCCACTGCTTCGGGAGGTGATAATCAACACGCGTTTTGTG AAAGGGGACATCAGCACTAAGTTTCTCTCTGATGTGTATCCTGATGGCTTCAAAGGGCACATGTTAACAC CCAGTGAGAGAGACCAGTTATTGGCTATTGCATCATCCTTGTTTGTGGCATCCCAGCTACGAGCACAGCG CTTTCAAGAACATTCAAGAGTTCCAGTTATAAGGCCAGATGTGGCTAAGTGGGAGCTCTCAGTAAAATTA CATGATGAAGACCATACTGTTGTGGCATCCAACAACGGGCCAACATTTAACGTGGAAGTTGATGGCTCGA AACTAAATGTGACCAGTACGTGGAACCTGGCCTCACCCTTATTGTCTGTCAACGTTGATGGCACTCAGAG GACTGTGCAGTGTCTTTCTCGGGACGCAGGTGGAAACATGAGCATCCAGTTTCTTGGCACAGTGTACAAA GTGCACATTTTAACCAAACTTGCTGCAGAACTGAACAAATTCATGCTTGAAAAAGTACCCAAGGACACTA GCAGCACTCTGTGCTCCCCGATGCCTGGAGTGGTGGTGGCCGTTTCCGTCAAGCCTGGAGACATGGTAGC AGAAGGTCAGGAAATCTGTGTGATTGAAGCTATGAAAATGCAGAACAGTATGACAGCTGGTAAAATGGGC AAGGTGAAACTGGTGCACTGCAAAGCTGGAGACACAGTTGGTGAAGGAGACCTGCTCGTGGAGCTGGAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR205851 representing NM_019330
Red=Cloning site Green=Tags(s) MAGLWVRTVALLAARRHWRRSSQQLLWTLKRAPVYSQQCLVVSRSLSSVEYEPKEKTFDKILIANRGEIA CRVIKTCRKMGIRTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTGAQAVHPGYGF LSENKEFAKCLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVLKDADEAVRIAREIGYP VMIKASAGGGGKGMRIPWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWL NERECSIQRRNQKVVEEAPSIFLDPETRRAMGEQAVAWPKAVKYSSAGTVEFLVDSQKNFYFLEMNTRLQ VEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPIH LPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDSYVIRGVTHNIPLLREVIINTRFV KGDISTKFLSDVYPDGFKGHMLTPSERDQLLAIASSLFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKL HDEDHTVVASNNGPTFNVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSRDAGGNMSIQFLGTVYK VHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKMG KVKLVHCKAGDTVGEGDLLVELE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019330 |
| ORF Size | 2169 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019330.1, NP_062203.1 |
| RefSeq Size | 2495 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 687008 |
| UniProt ID | P14882 |
| MW | 80 kDa |
| Gene Summary | alpha subunit of propionyl coenzyme A carboxylase, which catalyzes the conversion of ATP, propanoyl-CoA, and HCO3- to ADP, phosphate, and (S)-methylmalonyl-CoA [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205851 | Pcca (untagged ORF) - Rat propionyl-coenzyme A carboxylase, alpha polypeptide (Pcca), (10 ug) |
CNY 9,310.00 |
|
| RR205851L3 | Lenti ORF clone of Pcca (Myc-DDK-tagged ORF) - Rat propionyl-coenzyme A carboxylase, alpha polypeptide (Pcca), (10 ug) |
CNY 11,120.00 |
|
| RR205851L4 | Lenti ORF clone of Pcca (mGFP-tagged ORF) - Rat propionyl-coenzyme A carboxylase, alpha polypeptide (Pcca), (10 ug) |
CNY 12,160.00 |
