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Chrna2 (NM_133420) Rat Tagged ORF Clone
CAT＃: RR214926
- TrueORF®
Chrna2 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 2 (neuronal) (Chrna2), (10 ug)
"NM_133420" in other vectors (3)
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CNY 6,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 11,000.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACHR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214926 representing NM_133420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTTTCCCATTCTGCTCTCCAGTTCTGGACACATCTTTATCTCTGGTGTCTCCTTCTGGTGCCAG CAGTGTTGACCCAGCAAGGCTCACACACCCATGCTGAGGACCGCCTGTTCAAACACCTGTTTGGAGGCTA CAATCGCTGGGCACGGCCAGTGCCCAACACTTCTGATGTGGTCATCGTGCGCTTTGGATTATCCATTGCT CAGCTCATAGATGTGGATGAGAAGAATCAAATGATGACCACCAATGTCTGGCTAAAGCAGGAATGGAATG ACTACAAGCTGCGCTGGGACCCGGCTGAGTTTGGCAATGTCACCTCCCTGCGCGTCCCTTCAGAGATGAT CTGGATCCCAGACATTGTCCTCTACAACAATGCAGATGGGGAGTTTGCGGTGACCCACATGACCAAGGCT CACCTCTTCTTCACGGGCACTGTGCACTGGGTGCCCCCAGCCATCTACAAGAGCTCCTGCAGCATCGATG TGACCTTCTTCCCCTTCGACCAGCAGAACTGCAAGATGAAGTTTGGCTCCTGGACATATGACAAGGCCAA GATCGATCTGGAGCAGATGGAGAGGACAGTGGACCTGAAGGACTACTGGGAGAGTGGCGAGTGGGCCATT ATCAATGCCACCGGAACCTATAACAGTAAGAAGTACGACTGCTGCGCGGAGATCTACCCCGATGTCACCT ACTACTTTGTGATCCGCCGGCTGCCGCTGTTCTATACCATCAACCTCATCATCCCATGCCTGCTCATCTC CTGCCTCACTGTGCTCGTGTTCTACCTGCCTTCCGAGTGTGGAGAGAAGATCACGCTGTGCATCTCGGTG CTGCTATCTCTCACTGTCTTCCTGCTGCTCATCACGGAGATCATCCCGTCCACCTCGCTGGTCATCCCAC TCATCGGCGAGTACCTGCTCTTCACCATGATCTTTGTCACCCTCTCTATCGTTATCACAGTCTTCGTGCT CAATGTACACCACCGCTCCCCCAGCACCCACAACATGCCCAACTGGGTAAGGGTAGCCCTGCTAGGCCGG GTGCCCAGGTGGCTGATGATGAACCGGCCCCTGCCACCTATGGAGCTCCATGGCTCCCCGGATCTGAAGC TCAGCCCCTCATACCATTGGCTAGAGACTAACATGGATGCTGGAGAAAGGGAGGAGACAGAGGAAGAGGA GGAAGAAGAAGATGAAAACATATGTGTGTGTGCAGGCCTTCCAGACTCTTCGATGGGTGTCCTCTATGGC CATGGCGGCCTGCATCTGAGAGCCATGGAGCCTGAGACCAAGACTCCATCCCAGGCTAGCGAGATTCTGC TGTCACCTCAAATACAGAAAGCACTAGAAGGTGTACACTACATTGCTGACCGTCTGAGGTCTGAGGATGC TGACTCTTCGGTGAAGGAAGACTGGAAGTATGTGGCCATGGTGGTAGACCGGATATTCCTCTGGCTGTTC ATTATCGTCTGCTTCCTGGGGACCATCGGACTCTTCCTTCCTCCATTCCTGGCTGGAATGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214926 representing NM_133420
Red=Cloning site Green=Tags(s) MTLSHSALQFWTHLYLWCLLLVPAVLTQQGSHTHAEDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIA QLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNVTSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKA HLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAI INATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISV LLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGR VPRWLMMNRPLPPMELHGSPDLKLSPSYHWLETNMDAGEREETEEEEEEEDENICVCAGLPDSSMGVLYG HGGLHLRAMEPETKTPSQASEILLSPQIQKALEGVHYIADRLRSEDADSSVKEDWKYVAMVVDRIFLWLF IIVCFLGTIGLFLPPFLAGMI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133420 |
| ORF Size | 1533 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133420.1, NP_596911.1 |
| RefSeq Size | 1931 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 170945 |
| UniProt ID | P12389 |
| MW | 58.6 kDa |
| Gene Summary | agonist-binding subunit of neuronal nicotinic acetylcholine receptor [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Muscarinic M5receptors modulate ethanol seeking in rats
,Berizzi, AE;Perry, CJ;Shackleford, DM;Lindsley, CW;Jones, CK;Chen, NA;Sexton, PM;Christopoulos, A;Langmead, CJ;Lawrence, AJ;,
Neuropsychopharmacology
,PubMed ID 29483658
[CHRNA2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214926 | Chrna2 (untagged ORF) - Rat cholinergic receptor, nicotinic, alpha 2 (neuronal) (Chrna2), (10 ug) |
CNY 6,560.00 |
|
| RR214926L3 | Lenti ORF clone of Chrna2 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 2 (neuronal) (Chrna2), (10 ug) |
CNY 8,460.00 |
|
| RR214926L4 | Lenti ORF clone of Chrna2 (mGFP-tagged ORF) - Rat cholinergic receptor, nicotinic, alpha 2 (neuronal) (Chrna2), (10 ug) |
CNY 9,220.00 |
