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DBT (NM_001918) Human Untagged Clone
CAT＃: SC118943
DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCATE2; BCKAD-E2; BCKADE2; BCKDH-E2; BCOADC-E2; E2; E2B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001918, the custom clone sequence may differ by one or more nucleotides
ATGGCTGCAGTCCGTATGCTGAGAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTC AAACATGTGGTAATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTA TAGTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCAAGCTCTCA GACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAGAAGGAGATACAGTGTCTC AGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTGTTACCATCACTAGTCGTTATGATGGAGT CATTAAAAAACTCTATTATAATCTAGACGATATTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACG GAAGCTTTAAAAGATTCAGAAGAAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACC AAGAGATAAAGGGCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAA GCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTAAAGAAGATATCCTCAACTATTTGGAA AAGCAGACAGGAGCTATATTGCCTCCTTCACCCAAAGTTGAAATTATGCCACCTCCACCAAAGCCAAAAG ACATGACTGTTCCTATACTAGTATCAAAACCTCCGGTATTCACAGGCAAAGACAAAACAGAACCCATAAA AGGCTTTCAAAAAGCAATGGTCAAGACTATGTCTGCAGCCCTGAAGATACCTCATTTTGGTTATTGTGAT GAGATTGACCTTACTGAACTGGTTAAGCTCCGAGAAGAATTAAAACCCATTGCATTTGCTCGTGGAATTA AACTCTCCTTTATGCCTTTCTTCTTAAAGGCTGCTTCCTTGGGATTACTACAGTTTCCTATCCTTAACGC TTCTGTGGATGAAAACTGCCAGAATATAACATATAAGGCTTCTCATAACATTGGGATAGCAATGGATACT GAGCAGGGTTTGATTGTCCCTAATGTGAAAAATGTTCAGATCTGCTCTATATTTGACATCGCCACTGAAC TGAACCGCCTCCAGAAATTGGGCTCTGTGGGTCAGCTCAGCACCACTGATCTTACAGGAGGAACATTTAC TCTTTCCAACATTGGATCAATTGGTGGTACCTTTGCCAAACCAGTGATAATGCCACCTGAAGTAGCCATT GGGGCCCTTGGATCAATTAAGGCCATTCCCCGATTTAACCAGAAAGGAGAAGTATATAAGGCACAGATAA TGAATGTGAGCTGGTCAGCTGATCACAGAGTTATTGATGGTGCTACAATGTCACGCTTCTCCAATTTGTG GAAATCCTATTTAGAAAACCCAGCTTTTATGCTACTAGATCTGAAATGA >OriGene 5' read for NM_001918 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGTCCGTATGCTGA GAACCTGGAGCAGGAATGCGGGGAAGCTGATTTGTGTTCGCTATTTTCAAACATGTGGTA ATGTTCATGTTTTGAAGCCAAATTATGTGTGTTTCTTTGGTTATCCTTCATTCAAGTATA GTCATCCACATCACTTCCTGAAAACAACTGCTGCTCTCCGTGGACAGGTTGTTCAGTTCA AGCTCTCAGACATTGGAGAAGGGATTAGAGAAGTAACTGTTAAAGAATGGTATGTAAAAG AAGGAGATACAGTGTCTCAGTTTGATAGCATCTGTGAAGTTCAAAGTGATAAAGCTTCTG TTACCATCACTAGTCGTTATGATGGAGTCATTAAAAAACTCTATTATAATCTAGACGATA TTGCCTATGTGGGGAAGCCATTAGTAGACATAGAAACGGAAGCTTTAAAAGATTCAGAAG AAGATGTTGTTGAAACTCCTGCAGTGTCTCATGATGAACATACACACCAAGAGATAAAGG GCCGAAAAACACTGGCAACTCCTGCAGTTCGCCGTCTGGCAATGGAAAACAATATTAAGC TGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTANAGAAGATATCCTCAACT ATTTGGAAAAGCAGACAGGAGCTATATTGCCTNCTTCACCCAAGTTGAAATATGCCACCT NCACAAAGCCAAAGACATGACTGTCCTATACTAGTATCAAAACCTCCGTATCACAGGCAA GACAAACAGAACCATAAAAGGCTTCAAAAGCATGGTCAAGACTTGTCTGCAGCCTGAGAT ACCTATTTTGGTTATGNGAGAAATGACCTACTGACTGGNTAGCTCGAAAAAATAAACCCA TGCT >OriGene 3' read for NM_001918 unedited
AGACTCATGGTCGCTGCCGCAATCCANGATCAGTTTTTTTTTTTTTTTTTTGAGACAAAT GTTGCTCTGCCACCCAGGCTGGAGTGCAGTGGTGTGATCTCTGCTCACTGTAACCTCCAC CTCTTGGGCTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGTG TGCCACCAGGCCCAGGTAATTTTTTGTATTTTTACAAAACTGTAGAGATGGGGTTTCACC ATGTGGGCCAGGGTGGTCTCGAACTCCTGACCTCAGGTGATCTGGCCACCTGGGCCTCCC AAAGTGCTGGGATTACAGGCGTGAGCCACCAGGCTAATTTTAAAAATAATGTTTTTTGAG ATGGGATCTCACTATGTTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGTGATCCTCCT GGTTCAGCCTCCCCTATAACTGGGGATTACAGTTACAAGTCATCACACCTGGCTGATTTA TTCTTTCCTGATTTTAAAATTATTTCCATTTTGGGAAAAACCAAGTTGATTTTAAAACAA CCTTTTTTCTGGCACAAAACTTGCCTTAAACAACTAATTTTGGCATACAGGTTTCAACTG GACCAAATTAGAAACAACTGGGATTTCCGAAAGCCAATTGGAATGGTGGAACAATTATCC CGGTCCTTTTTCTGAGTAAGTGGACCTTTTACAAAATGGTTCTTTGTGAAACCTTCGTTT CCATACAAAAAGTCTCATTTTTTTTATCCTTAATACCATTAAGCTTTTCCTTCTCCTGGT AAGGAACATTTTACCCCGGCATCTGGGTAAATGAATAGACCCTAATTTCATAAACCGATT TTTAAGGCGGAGACCCCATAAGTTAATATGGGAGTGCGCTTCTCTCATTTTTTTGATAAA AACAAAATCGGTGGGTGCCTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001918 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001918.1, NP_001909.1 |
| RefSeq Size | 10831 bp |
| RefSeq ORF | 1449 bp |
| Locus ID | 1629 |
| UniProt ID | P11182 |
| Domains | biotin_lipoyl, 2-oxoacid_dh, e3_binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201998 | DBT (Myc-DDK-tagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC201998L1 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201998L2 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
| RC201998L3 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,180.00 |
|
| RC201998L4 | Lenti ORF clone of Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,180.00 |
|
| RG201998 | DBT (tGFP-tagged) - Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
| SC322453 | DBT (untagged)-Human dihydrolipoamide branched chain transacylase E2 (DBT), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
