FUBP1 (BC017247) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNA helicase V; far upstream element (FUSE) binding protein 1; far upstream element-binding protein; far upstream element binding protein; FBP; FUBP; FUSE-binding protein; OTTHUMP00000038483
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017247 edited TCTTTCTTAGCTGTTAGCTGAGAGGAAGTCTCTGAACAGGCGGCAGCGGCTCTTATAGTG CAACCATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTG GTGGCGGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCC GGCAGATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATG GTTATGGGGGACAAAAAAGACCTTTAGAAGATGGAGATCAACCAGATGCTAAGAAAGTTG CTCCTCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGATCTG TAATGACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGAG GTGAACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACA GTGGTGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAG CAAAACGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATG GCGATGGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTAG TCATTGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATGG TTATGATTCAAGACGGGCCGCAGAACACTGGTGCTGACAAACCTCTTAGGATTACAGGAG ACCCATATAAAGTTCAACAAGCCAAGGAAATGGTGTTAGAGTTAATTCGTGATCAAGGCG GTTTCAGAGAAGTTCGGAATGAGTATGGGTCAAGAATAGGAGGAAATGAAGGGATAGATG TCCCCATTCCAAGATTTGCTGTTGGCATTGTAATAGGAAGAAATGGAGAGATGATCAAAA AAATACAAAATGATGCTGGTGTTCGCATTCAGTTTAAGCCAGATGATGGGACAACACCCG AAAGGATAGCACAAATAACAGGACCTCCAGACCGATGTCAACATGCTGCAGAAATTATTA CAGACCTTCTTCGAAGTGTTCAGGCTGGTAATCCTGGTGGACCTGGACCTGGTGGTCGAG GAAGAGGTAGAGGTCAAGGCAACTGGAACATGGGACCACCTGGTGGACTACAGGAATTTA ATTTTATTGTGCCAACTGGGAAAACTGGATTAATAATAGGAAAAGGAGGTGAAACCATAA AAAGCATAAGCCAGCAGTCTGGTGCAAGAATAGAACTTCAGAGAAATCCTCCACCAAATG CAGATCCTAATATGAAGTTATTTACAATTCGTGGCACTCCACAACAGATAGACTATGCTC GGCAACTCATAGAAGAAAAGATTGGTGGCCCAGTAAATCCTTTAGGGCCACCTGTACCCC ATGGGCCCCATGGTGTCCCAGGCCCCCATGGACCTCCTGGGCCTCCAGGGCCTGGAACTC CAATGGGACCATACAACCCTGCACCTTATAATCCTGGACCACCAGGCCCGGCTCCTCATG GTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCATATCCACACTGGCAGCAGC AGGCTCCTCCTGATCCAGCTAAGGCAGGAACGGATCCAAATTCAGCAGCTTGGGCTGCTT ATTACGCTCACTATTATCAACAGCAAGCACAGCCACCACCAGCAGCCCCTGCAGGTGCAC CAACTACAACTCAAACTAATGGACAAGGAGATCAGCAGAATCCAGCCCCAGCTGGACAGG TTGATTATACCAAGGCTTGGGAAGAGTACTACAAGAAAATGGGTCAGGCAGTTCCTGCTC CGACTGGGGCTCCTCCAGGTGGTCAGCCAGATTATAGTGCAGCCTGGGCTGAGTATTATA GACAACAAGCAGCCTATTATGCCCAGACAAGTCCCCAGGGAATGCCACAGCATCCTCCAG CACCTCAGTGCAGGTTTGATCCAGCCAGTATAGAACTAGCTCTGTAGGGGTGAGGAGGAC TGTGCTGTGTATCATCCTTGATTGTGTTCCTTCAAGGAGCATTGCACTTGCCTTCCCAGA CCTTCCACCTTAGGTTCTGCTGCAAAAAGCACCAGGTAAGCACAACCTAAGGACATATAT AAATAAATATTTCAATACATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017247 unedited GTCCGGACAGAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGTCTT TCTTAGCTGTTAGCTGAGNAGAAGTCTCTGAACAGGCGGCAGCGGCTCTTATAGTGCAAC CATGGCAGACTATTCAACAGTGCCTCCCCCCTCTTCTGGCTCAGCTGGTGGCGGTGGTGG CGGCGGTGGTGGTGGAGGAGTTAACGACGCTTTCAAAGATGCACTGCAGAGAGCCCGGCA GATTGCAGCAAAAATTGGAGGTGATGCAGGGACATCACTGAATTCAAATGACTATGGTTA TGGGGGACAAAAAAGACCTTTAGAAGATGGAGATCAACCAGATGCTAAGAAAGTTGCTCC TCAAAATGACTCTTTTGGAACACAGTTACCACCGATGCATCAGCAGCAAAGATCTGTAAT GACAGAAGAATACAAAGTTCCAGATGGAATGGTTGGATTCATAATTGGCAGAGGAGGTGA ACAGATCTCACGCATACAACAGGAATCTGGATGCAAAATACAGATAGCTCCTGACAGTGG TGGCCTTCCAGAAAGGTCCTGTATGTTAACTGGAACACCTGAATCTGTCCAGTCAGCAAA ACGGTTACTGGACCAGATTGTTGAAAAAGGAAGACCAGCTCCTGGCTTCCATCATGGCGA TGGACCGGGAAATGCAGTTCAAGAAATCATGATTCCAGCTAGCAAGGCAGGATTAGTCAT TGGAAAAGGGGGAGAAACTATTAAACAGCTTCAGGAACGGGCTGGAGTTAAAATGGTTAT GATTCAAGACGGGCCGCAGAACACTGGTGCTGANCAACCTCTTAGGATTACAGGAGACCC ATATAAAGTTCAACAAGCCAAGGAAATGGTGGTTAGAGTTAATTCGTGATCAAGGCGA
Restriction Sites	Please inquire
ACCN	BC017247
Insert Size	2249 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC017247.2, AAH17247.1
RefSeq Size	2240 bp
Locus ID	8880
Protein Families	Stem cell - Pluripotency, Transcription Factors
Gene Summary	The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.