MTHFD2 (BC015062) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	methylenetetrahydrofolate dehydro; methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; methylene tetrahydrofolate dehydrogenase 2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NMDMC
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015062 edited GGGGCCCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATGTCTGCTTTGG CTGCCCGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCG CGGCAGTTCGGGGAATCTCTCCCTAGGTCAGGTTGGAGTGCAGTGCTGCAATCACGGCTT ACTGCAGCCTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCTTAAATGAAGCTGT TGTCATTTCTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGA AGAGTGGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGGTTGGCGAGAA TCCTGCAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAA CAGTGAGACAATTATGAAACCAGCTTCAATTTCAGAGGAAGAATTGTTGAATTTAATCAA TAAACTGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCA TATTGATGAGAGAAGGATCTGCAATGCTGTTTCTCCAGACAAGGATGTTGATGGCTTTCA TGTAATTAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGGCTACTCCATG GGGTGTGTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAAGAATGTGGTTGT GGCTGGAAGGTCAAAAAACGTTGGAATGCCCATTGCAATGTTACTGCACACAGATGGGGC GCATGAACGTCCCGGAGGTGATGCCACTGTTACAATATCTCATCGATATACTCCCAAAGA GCAGTTGAAGAAACATACAATTCTTGCAGATATTGTAATATCTGCTGCAGGTATTCCAAA TCTGATCACAGCAGATATGATCAAGGAAGGAGCAGCAGTCATTGATGTGGGAATAAATAG AGTTCACGATCCTGTAACTGCCAAACCCAAGTTGGTTGGAGATGTGGATTTTGAAGGAGT CAGACAAAAAGCTGGGTATATCACTCCAGTTCCTGGAGGTGTTGGCCCCATGACAGTGGC AATGCTAATGAAGAATACCATTATTGCTGCAAAAAAGGTGCTGAGGCTTGAAGAGCGAGA AGTGCTGAAGTCTAAAGAGCTTGGGGTAGCCACTAATTAACTACTGTGTCTTCTGTGTCA CAAACAGCACTCCAGGCCAGCTCAAGAAGCAAAGCAGGCCAATAGAAATGCAATATTTTT AATTTATTCTACTGAAATGGTTTAAAATGATGCCTTGTATTTATTGAAAGCTTAAATGGG TGGGTGTTTCTGCACATACCTCTGCAGTACCTCACCAGGGAGCATTCCAGTATCATGCAG GGTCCTGTGATCTAGCCAGGAGCAGCCATTAACCTAGTGATTAATATGGGAGACATTACC ATATGGAGGATGGATGCTTCACTTTGTCAAGCACCTCAGTTACACATTCGCCTTTTCTAG GATTGCATTTCCCAAGTGCTATTGCAATAACAGTTGATACTCATTTTAGGTACCAAACCT TTTGAGTTCAACTGACCAAACCAAAGGAAAAGTGTTGCTAGAGAAAATTAGGGAAAAGGT GAAAAAGAAAAAATGGTAGTAATTGAGCAGAAAAAAATTAATTTATATATGTATTGATTG GCAACCAGATTTATCTAAGTAGAACTGAATTGGCTAGGAAAAAAGAAAAACTGCATGTTA ATCATTTTCCTAAGCTGTCCTTTTGAGGCTTAGTCAGTTTATTGGGAAAATGTTTAGGAT TATTCCTTGCTATTAGTACTCATTTTATGTATGTTACCCTTCAGTAAGTTCTCCCCATTT TAGTTTTCTAGGACTGAAAGGATTCTTTTCTACATTATACATGTGTGTTGTCATATTTGG CTTTTGCTATATACTTTAACTTCATTGTTAAATTTTTGTATTGTATAGTTTCTTTGGTGT ATCTTAAAACCTATTTTTGAAAAACAAACTTGGCTTGATAATCATTTGGGCAGCTTGGGT AAGTACGCAACTTACTTTTCCACCAAAGAACTGTCAGCAGCTGCCTGCTTTTCTGTGATG TATGTATCCTGTTGACTTTTCCAGAAATTTTTTAAGAGTTTGAGTTACTATTGAATTTAA TCAGACTTTCTGATTAAAGGGTTTTCTTTCTTTTTTAATAAAACACATCTGTCTGGTATG GTACCAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015062 unedited AGCCAGAATTGTAAACGACTCACTATAGGCGGCCGCGTAATCGGCCATTACGGCCGGGGC CCGGCGCAGTCACCGGCGCGGTCTATGGCTGCGACTTCTCTAATGTCTGCTTTGGCTGCC CGGCTGCTGCAGCCCGCGCACAGCTGCTCCCTTCGCCTTCGCCCTTTCCACCTCGCGGCA GTTCGGGGAATCTCTCCCTAGGTCAGGTTGGAGTGCAGTGCTGCAATCACGGCTTACTGC AGCCTTGACCTCCTGGGCTCAAGTGATCCTCCCACCTCAGCTTAAATGAAGCTGTTGTCA TTTCTGGAAGGAAACTGGCCCAGCAGATCAAGCAGGAAGTGCGGCAGGAGGTAGAAGAGT GGGTGGCCTCAGGCAACAAACGGCCACACCTGAGTGTGATCCTGGTTGGCGAGAATCCTG CAAGTCACTCCTATGTCCTCAACAAAACCAGGGCAGCTGCAGTTGTGGGAATCAACAGTG AGACAATTATGAAACCAGCTTCAATTTCAGAGGAAGAATTGTTGAATTTAATCAATAAAC TGAATAATGATGATAATGTAGATGGCCTCCTTGTTCAGTTGCCTCTTCCAGAGCATATTG ATGAGAGAAGGATCTGCAATGCTGTTTCTCCAGACAGGATGTTGATGGCTTTCATGTAAT TAATGTAGGACGAATGTGTTTGGATCAGTATTCCATGTTACCGGCTACTCCATGGGGTGT GTGGGAAATAATCAAGCGAACTGGCATTCCAACCCTAGGGAACAATGTGGTTGTGGCCTG AACGTCAAAAAACGTTGGAATGCCCATTGCAATGTTACTGCACACAGATGGGGCGCATGA ACGTCCCGGGAGTGATGCCACTGTTANCATATCTCATCGATATACTCCCAAAAAGCAGTT GAAGAAACATACTATTCGTGCAGATATTGATATATCTGCTGCAGGT
Restriction Sites	NotI-NotI
ACCN	BC015062
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC015062.1, AAH15062.1
RefSeq Size	2250 bp
Locus ID	10797
Protein Families	Druggable Genome
Protein Pathways	Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
Gene Summary	This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

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