BAAT (NM_001127610) Human Untagged Clone

CAT#: SC323038

BAAT (untagged)-Human bile acid CoA: amino acid N-acyltransferase (glycine N-choloyltransferase) (BAAT), transcript variant 2



  "NM_001127610" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-BAAT Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms BACAT; BACD1; BAT; HCHO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC323038 representing NM_001127610.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATCCAGTTGACAGCTACCCCTGTGAGTGCACTTGTTGATGAGCCAGTGCATATCCGAGCTACAGGC
CTGATTCCCTTTCAGATGGTGAGTTTTCAGGCATCACTGGAAGATGAAAACGGAGACATGTTTTATTCT
CAAGCCCACTATAGGGCCAATGAATTCGGTGAGGTGGACCTGAATCATGCTTCTTCACTTGGAGGGGAT
TATATGGGAGTCCACCCCATGGGTCTCTTCTGGTCTCTGAAACCTGAAAAGCTATTAACAAGACTGTTG
AAAAGAGATGTGATGAATAGGCCTTTCCAGGTCCAAGTAAAACTTTATGACTTAGAGTTAATAGTGAAC
AATAAAGTTGCCAGTGCTCCAAAGGCCAGCCTGACTTTGGAGAGGTGGTATGTGGCACCTGGTGTCACA
CGAATTAAGGTTCGAGAAGGCCGCCTTCGAGGAGCTCTCTTTCTCCCTCCAGGAGAGGGTCTCTTCCCA
GGGGTAATTGATTTGTTTGGTGGTTTGGGTGGGCTGCTTGAATTTCGGGCCAGCCTCCTAGCCAGTCGT
GGCTTCGCCTCCTTGGCCTTGGCTTACCATAACTATGAAGACCTGCCCCGCAAACCAGAAGTAACAGAT
TTGGAATATTTTGAGGAGGCTGCCAACTTTCTCCTGAGACATCCAAAGGTCTTTGGCTCAGGCGTTGGG
GTAGTCTCTGTATGTCAAGGAGTACAGATTGGACTATCTATGGCTATTTACCTAAAGCAAGTCACAGCC
ACGGTACTTATTAATGGGACCAACTTTCCTTTTGGCATTCCACAGGTATATCATGGTCAGATCCATCAG
CCCCTTCCCCATTCTGCACAATTAATATCCACCAATGCCTTGGGGTTACTAGAGCTCTATCGCACTTTT
GAGACAACTCAAGTTGGGGCCAGTCAATATTTGTTTCCTATTGAAGAGGCCCAGGGGCAATTCCTCTTC
ATTGTAGGAGAAGGTGATAAGACTATCAACAGCAAAGCACACGCTGAACAAGCCATAGGACAGCTGAAG
AGACATGGGAAGAACAACTGGACCCTGCTATCTTACCCTGGGGCAGGCCACCTGATAGAACCTCCCTAT
TCTCCTCTGTGCTGTGCCTCAACGACCCACGATTTGAGGTTACACTGGGGAGGAGAGGTGATCCCACAC
GCAGCTGCACAGGAACATGCTTGGAAGGAGATCCAGAGATTTCTCAGGAAGCACCTCATTCCAGATGTG
ACCAGTCAACTCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001127610
Insert Size 1257 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001127610.1
RefSeq Size 3377 bp
RefSeq ORF 1257 bp
Locus ID 570
UniProt ID Q14032
Protein Pathways Biosynthesis of unsaturated fatty acids, Metabolic pathways, Primary bile acid biosynthesis, Taurine and hypotaurine metabolism
MW 46.3 kDa
Gene Summary The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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