NUP62 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	NUP62
Locus ID	23636
Kit Components	KN204418G1, NUP62 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCTACAGGCGGGTTCACGTT KN204418G2, NUP62 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCAACAACCACACCTGCTAC KN204418-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGCCTGGGGG GCCTTCAGCA ACCCAGACCC TGCCATCCAG CCTGGGTTCC CGGCCCCATC TCTCTCTCTG AGGTCCAGAG AGGCCCTCAG GGTGGCAAGA GCTGATCCGC TCACACCCTG CTAGAGTCAG CCAGGGAACC TCACTGGGTC CCACGCTGGG AGCAGGTCCT ACAGCTGTTC TTGTGTGTCC TAGTCCCGCT TGTGGCAGTG CCTCAAATCT GATCTCCTCA GGCTGAGGTG GGTCCAAGGT CCTGTTCTCC CGCCTCGTCC TCCACAGAGT GGGAGCTTTC CACTGGGCTG AACATGGCCA CGCACGTCCC TAGCACCCCG GCTCCTCGTA CCTGACCCCT GGGGCGTGCT TTTGTGCTTC TGGATCCTTC TCGATGTAGC AGAGCCTAGT TTAGGTTACA CAGCATGCAT CATTATCCCA TCCCACCACC CTCCAAACCG ACTGTGCCCA GTCATGCCAC TGAGCTTGCC TTTCCAAAAC TGCCATCTGA CAATGTCACT TCCTCTGCTT TAGAAATCTT CCCAAGGCTG CAGACACCGA CGGATTTGCT TTGGGAGCCA GAGTAGCTGC CGCCACCAGA GTCCGGAGCC CCAGCCACAA GTACCCCTTC CACCGGCCTG TTCTCACTTG CCACCCAGAC TCCGGCCACA CAGACGACAG GCTTCACTTT TGGAACAGCG ACTCTTGCTT CGGGGGGAAC TGGATTTTCT TTGGGGATCG GTGCTTCAAA GCTCAACTTG AGCAACACAG CTGCCACCCC AGCCATGGCA AACCCCAGCG GCTTTGGGCT GGGCAGCAGC AACCTCACTA ATGCCATATC GAGCACCGTC ACCTCCAGCC AGGGCACAGC ACCCACCGGC TTTGTGTTTG GCCCCTCCAC CACCTCTGTG GCTCCAGCTA CCACATCTGG AGGCTTCTCA TTCACTGGTG GAAGCACGGC CCAACCCTCC GGTTTCAACA TTGGCTCAGC AGGGAATTCA GCCCAGCCCA CGGCACCTGC CACGTTGCCC TTCACTCCGG CCACGCCAGC AGCCACCACA GCAGGTGCCA CACAGCCAGC TGCTCCCACA CCCACAGCCA CCATCACCAG CACTGGGCCC AGCCTCTTTG CGTCAATAGC AACTGCTCCA ACCTCATCTG CCACCACTGG ACTCTCCCTC TGTACCCCTG TGACCACAGC GGGCGCCCCC GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001193357, NM_012346, NM_016553, NM_153718, NM_153719
Synonyms	IBSN; p62; SNDI
Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]

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