P cadherin (CDH3) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
Donor DNA	mBFP-Neo
Symbol	P cadherin
Locus ID	1001
Kit Components	KN207346G1, P cadherin gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAGACGCGAGAGGTCCACGA KN207346G2, P cadherin gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCAGGGAGCGGTCCCGGTCG KN207346BN-D, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette. Homologous arm and mBFP-Neo sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; mBFP-Neo in green; Right arm in violet GAAACGCGGT CCTTCGGGAC CTGCTAGTTT TATACCCCGG AGGATCCTCC CCGGCGGGCT GGCACGGGAG GTGGAGAAAG AGGCTTGGGC GGCCCCGCTG TAGCCGCGTG TGGGAGGACG CACGGGCCTG CTTCAAAGCT TTGGGATAAC AGCGCCTCCG GGGGATAATG AATGCGGAGC CTCCGTTTTC AGTCGACTTC AGATGTGTCT CCACTTTTTT CCGCTGTAGC CGCAAGGCAA GGAAACATTT CTCTTCCCGT ACTGAGGAGG CTGAGGAGTG CACTGGGTGT TCTTTTCTCC TCTAACCCAG AACTGCGAGA CAGAGGCTGA GTCCCTGTAA AGAACAGCTC CAGAAAAGCC AGGAGAGCGC AGGAGGGCAT CCGGGAGGCC AGGAGGGGTT CGCTGGGGCC TCAACCGCAC CCACATCGGT CCCACCTGCG AGGGGGCGGG ACCTCGTGGC GCTGGACCAA TCAGCACCCA CCTGCGCTCA CCTGGCCTCC TCCCGCTGGC TCCCGGGGGC TGCGGTGCTC AAAGGGGCAA GAGCTGAGCG GAACACCGGC CCGCCGTCGC GGCAGCTGCT TCACCCCTCT CTCTGCAGCC ATGGGGCAGT GGCGTCGGGG AGAGCGCGGG GCTGCGCTCC CTGGGGCCAA GGGAGTCCCG GAAGGCCCGT GAGGACCCTG CGGTGTGGGG AGTGCAGGGC CGGGCACGCC TGGACCCAGC CTCCTTCACT CTCTGCCCTC GGGCGCAGGT TTGCTGGCTG CAGTGCGCGG CCTCCGAGCC GTGCCGGGCG GTCTTCAGGG AGGCTGAAGT GACCTTGGAG GCGGGAGGCG CGGAGCAGGA GCCCGGCCAG GCGCTGGGGA AAGGTAAGAT CCTCAGGGTG GAACCGCAGG GTAGGGGCCG CACGTGACCA TTTTGGTGTG GACCGCGCGA GGGGTGTTCG GGCCGGGGCA AGGGACTCCT GGCGCCACCT CAGTTCCCGG GGAGGCGCAG AACGCGCCTC GGGGGCTGGG ATTGGGGGTG GTGGCTGACG GAGAAGGCTG GGCAGCAGAG CGCCTATGAC ATCCAGCCCC TTGTCCGGTA GCCACTGCTT ACCTTGACCC CCTCCTCCGA GATGCCGGAT GGCTGAGCCC CTCACCCAGT CTCGACGTGG GAAGTTCTCC CCCGCTGGCC CCAGCAGAGG AATGCGCCGG CCACATTCTT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001317195, NM_001317196, NM_001793
Synonyms	CDHP; HJMD; PCAD
Summary	This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]

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