CHD7 Human Gene Knockout Kit (CRISPR)

CAT#: KN221060

CHD7 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit Polyclonal CHD7 Antibody
    • 100 ug

CNY 5,808.00


CHD7 (tGFP-tagged) - Human chromodomain helicase DNA binding protein 7 (CHD7)
    • 10 ug

CNY 30,728.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol CHD7
Locus ID 55636
Kit Components

KN221060G1, CHD7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGAGTCTTTTTGGCGAGGAT

KN221060G2, CHD7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CCTCGGAGAATGTGGTTACC

KN221060-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGGTTAAGG GCGGGCTGTT GGTAACCAGA GCATTTCCAG GGCTTTAGTT CAGCAGATGA GCTGCTTGGC TATATTCATA CATCTGCTTG CCTGCTGTGG CAGCTGCTGC TGCTTCACGT TGTGTTCTTT TGTTCTGTTT CATCCAAGAA TGGAACGCTA GGAAGTGTAG TGAATGGATT AATTTAAGGT TAAGGTTAGT GTTTTGCTTG CTTTCTAAGT AACCGATCAT AATGATGCTT CTGGTAGAAG CTAATGAGTT TCTCTTAAAG TGAACTAAAT TTATAAAATA CAGCTCAGCG AGGGAGCTAG ATTCGTATTG TTGATGGGGA CCAAATTCAG ATGTACAAAA TACTGGCAGT TTTTATCATT GGATCCTAAT TCTTTGTTTT CTTCCTTCTT CTCCCCCACC CCAAACTCCC TTCCAGGACC TATATCCAGA CTTTGCCTGA CACTGCAGGG TCCAAGAGAA TTAAAGAAAT ATGGAATGAC ATGAAGAAGA TTAGTTAAGG ATTATAGGCT TTGAGGGCAA ACACCTCAGT GAAGTGAAGC ACAGGCAAGC TCCTGAGCTG TGGTTTGGAG GAGCCGTGTG TTGGAAGAAG GAAAATCCAG TAAATCCTAT GGGTCAGCAA ATGCCAATAG ACCAAGGCTT TGCCTCTTTA CAGCCATCCC TTCATCATCC TTCAACTAAT CAAAATCAAA CAAAGCTGAC ACATTTTGAT CACTATAATC AGTATGAACA ACAAAAGATG CATCTGATGG ATCAGCCGAA CAGAATGATG AGCAACACCC CTGGGAACGG ACTCGCGTCT CCGCACTCGC AGTATCACAC CCCTCCCGTT CCTCAGGTGC CCCATGGTGG CAGTGGTGGC GGTCAGATGG GTGTCTACCC TGGCATGCAG AATGAGAGGC ATGGGCAATC CTTTGTGGAC AGCAGCTCCA TGTGGGGCCC CAGGGCTGTT CAGGTACCAG ACCAGATACG AGCCCCCTAC CAGCAGCAGC AGCCACAGCC GCAGCCACCG CAGCCGGCTC CGTCGGGGCC CCCTGCACAG GGCCACCCTC AGCACATGCA GCAGATGGGC AGCTATATGG CACGTGGGGA TTTTTCCATG CAGCAGCATG GTCAGCCACA GCAGAGGATG AGCCAGTTTT CCCAAGGCCA AGAGGGCCTC AATCAGGGAA ATCCTTTTAT TGCCACCTCA

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001316690, NM_017780, NM_017783
Synonyms CRG; HH5; IS3; KAL5
Summary This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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