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PD-L1 (Cd274) Mouse Gene Knockout Kit (CRISPR)
CAT＃: KN302893RB
Cd274 - mouse gene knockout kit via CRISPR, HDR mediated
HDR-mediated knockout kit validation
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Format | 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control |
| Donor DNA | RFP-BSD |
| Symbol | PD-L1 |
| Locus ID | 60533 |
| Kit Components |
KN302893G1, PD-L1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCCTGCTGTCACTTGCTACG KN302893G2, PD-L1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TAGAACCCACTGAAAAGATT KN302893RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACAGG CTGTCGCCGT GCTCATTTGA TAACTGCCCG TTATTCATGC GACACACCGG GCCAGATGGA AGTCTCCTTC CTTCCCATAG CTGTTACCAC TGTACTGTCT TTACTGGGGA AAAGATTTAT CATGTGGAAT AGGTGCGAGG CAGAGGTGAG ATTTTAATGG GGAGGAAGCA TTGAAAAGTG AAAGTGAAAA TTGGATGCTC TTTGCTTTGA AGCTTTGCCT AAAGCAGGTT TTAGCTTTCA AATACGTTTC AATGTTGAAA GAACGCATGA TACATATGGA GGGGGGCCTG GGGGGGGTCC TTGGCTGAGT TTGAATGTAC ATTAACAATC TGGGGGGCTA ATAACTCAAT GTAAAGCTGC TGATCCCATC ATACTGACTT CTTTCCACTT GGTTCTACAT GGCTTTGAGT TACAAAATGA AAGCATTGAA TTTTGAACTG TTCAGCTGTG TTTCCACACT TGCAAATCGG TTGTTGGCCA GCCCTCAGAA TTGCTTCAGT TACAGCTGGC TCGTCTGCTC TTTCCAGACT GGCTTTTAGG GCTTATGTAT ATATGAGAAG GACACATTTA CTAGTGTCTC CTTGCTCTGC TATTGAAATT AAGCAGACCT CTCTGTGTTT CCCGTTACTA GATAGTTCCC AAAACCAATA TTTTAGCTAT GAATTAAAAA TGGAAGTAAT TTGTGGGGTG TGTATGTGTG TGTATATGTG TGTGTAGAGG GGGGTCTGTG TGTATGTGCA GTTGCTAGGC ACACATAAAG CGTTCATAGG ACAACCTAGA GCTTAGTCCT CACCTTCTAC CTTGTTTGAG ACAAGGTCTC TTATTTGTTG TACATTGCTG AGTCCTGTAG TTCGGCTAGC TCAGAACCTC CTGGGGGCTC TCCTGTCTCC ACCTCCCAGT CCACTGAGAT TGTAGGCACA TGCTACTGCA CCTGGCTTCT ACCTGGTCTC TGGGGATTTG AACTTGGGTC CATGGGCTAC ACAGCAAGTC GTTTACTTAC TGGGCAATCA CTCCATCCCC TAAGATAATT ATAAGGAATA TACCTTGCTT ATCCAAACAC ATTCTCATTC TCCTTTGCCA TAAATAAGTT ACTTGGCAAA TATATTGTAT GTATTTTTAA TAAATAAATA AAATCTTAAA AATAAATAAA ATTATTTGTG AAGACAAAAA AAATAAGTTA CTTGGAAAGG ATGAAGGAAA ATACTGGAGC TTTGGGTGTG GTTTAGTAGT AGAACACTTG GCTGATCACT CTCGCCGGTT GGACTTTAGA TCAGAAGGGA TCTTGCTGCC GCCCGAAAGA GGAAGGGCTG GAAGAGGAAG GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATCGE100003, scramble sequence in pCas-Guide vector |
| Disclaimer | These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process. |
| Reference Data | |
| RefSeq | NM_021893 |
| Synonyms | A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1 |
| Summary | The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015] |
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| 基因表达相关资源 |
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| KN302893 | Cd274 - mouse gene knockout kit via CRISPR, HDR mediated |
CNY 12,260.00 |
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| KN302893BN | Cd274 - mouse gene knockout kit via CRISPR, HDR mediated |
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| KN302893LP | Cd274 - mouse gene knockout kit via CRISPR, HDR mediated |
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| KN502893 | Cd274 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated. |
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| GA206402 | Cd274 CRISPRa kit - CRISPR gene activation of mouse CD274 antigen |
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