RPIA (NM_144563) Human Mass Spec Standard

Specifications

Product Data
Description	RPIA MS Standard C13 and N15-labeled recombinant protein (NP_653164)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC202183
Predicted MW	33.1 kDa
Protein Sequence	Sequence Link (showhide) >RC202183 representing NM_144563 Red=Cloning site Green=Tags(s) MQRPGPFSTLYGRVLAPLPGRAGGAASGGGGNSWDLPGSHVRLPGRAQSGTRGGAGNTSTSCGDSNSICP APSTMSKAEEAKKLAGRAAVENHVRNNQVLGIGSGSTIVHAVQRIAERVKQENLNLVCIPTSFQARQLIL QYGLTLSDLDRHPEIDLAIDGADEVDADLNLIKGGGGCLTQEKIVAGYASRFIVIADFRKDSKNLGDQWH KGIPIEVIPMAYVPVSRAVSQKFGGVVELRMAVNKAGPVVTDNGNFILDWKFDRVHKWSEVNTAIKMIPG VVDTGLFINMAERVYFGMQDGSVNMREKPFC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_653164
RefSeq Size	1834
RefSeq ORF	933
Synonyms	RPI; RPIAD
Locus ID	22934
Cytogenetics	2p11.2
Summary	The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18. [provided by RefSeq, Mar 2010]
Protein Pathways	Metabolic pathways, Pentose phosphate pathway

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