Filamin Chicken Protein

Specifications

Product Data
Description	Filamin chicken protein, 0.25 mg
Species	Chicken
Protein Source	Gizzard
Predicted MW	250 kDa
Concentration	lot specific
Purity	>90% > 90% (determined by SDS gelelectrophoresis)
Buffer	Presentation State: Purified State: Lyophilized Buffer System: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl
Reconstitution	Restore with 250 µl Laemmli buffer (final volume 250 µl) or 9M urea buffer (e.g. IEF or NEPHGE buffer) Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step.
Preparation	Lyophilized
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization.
Protein Description	Filamin, purified polypeptide
Note	Isoelectric Point: pI 6.4
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the protein undiluted at -70°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.