Streptavidin (Beta-Galactosidase conjugated) Protein

Specifications

Product Data
Description	Streptavidin (Beta-Galactosidase conjugated) protein, 1 mg
Protein Source	S. avidinii
Concentration	lot specific
Purity	Prepared from electrophoretically pure Streptavidin.
Buffer	Presentation State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction. Buffer System: 0.02 M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, 0.01 M Magnesium Chloride and 0.01 M Beta-Mercaptoethanol, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Beta-Galactosidase (E. coli, MW 540, 000 daltons)
Preparation	Liquid (sterile filtered) purified Ig fraction.
Applications	Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature. Recommended Dilutions: ELISA: 1/8000-1/32000. Western Blot: 1/500-1/2500. Immunohistochemistry: 1/200-1/1000.
Note	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- beta galactosidase (E.coli) and anti-Streptavidin. No reaction was observed against anti-Avidin.
Storage	Store vial at 2-8°C before opening. DO NOT FREEZE! This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Freezing beta galactosidase conjugates will result in a substantial loss of enzymatic activity.
Stability	Shelf life: one year from despatch.
Reference Data

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