Biotin (Glucose Oxidase conjugated) Protein

Specifications

Product Data
Description	Biotin conjugated with Glucose Oxidase, 5 mg
Concentration	lot specific
Purity	This product was prepared from chromatographically purified biotin.
Buffer	State: Lyophilized purified Ig fraction. Buffer System: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 without preservatives. Label: Glucose Oxidase (GO) Presentation Label: Glucose Oxidase
Reconstitution	Restore with deionized water (or equivalent).
Preparation	Lyophilized purified Ig fraction.
Applications	Suitable for immunoblotting (western: 1/500-1/2,500 or dot blot), ELISA (1/8,000-1/32,000), immunoperoxidase electron microscopy and immunohistochemistry (1/200-1/1,000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
Protein Description	Glucose Oxidase Conjugated Biotin.
Storage	Store vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

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