Rat IgG (Fc Fragment) Rat Protein

Specifications

Product Data
Description	Rat IgG Fc Fragment rat protein, 1 mg
Species	Rat
Protein Source	Serum
Concentration	lot specific
Purity	This product was prepared from normal serum Delipidation, Salt Fractionation, Ion Exchange Chromatography followed by papain digestion and extensive dialysis against the buffer.
Buffer	State: Lyophilized purified Ig fraction. Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molar Ratio: 10-20 BAC molecules per Rat IgG F(c) molecule. Presentation Label: Biotin
Reconstitution	Restore with 1.0 ml of deionized water (or equivalent).
Preparation	Lyophilized purified Ig fraction.
Applications	Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
Protein Description	Biotin Conjugated Rat IgG F(c) fragment
Storage	Store (undiluted) at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Stability	Shelf life: one year from despatch. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Reference Data

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