Rat IgG (Fc Fragment) Rat Protein

Specifications

Product Data
Description	Rat IgG Fc Fragment rat protein, 1 mg
Species	Rat
Protein Source	Serum
Concentration	lot specific
Purity	Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Preservative: 0.01% (w/v) Sodium Azide
Reconstitution	Restore with 1.0 ml of deionized water (or equivalent).
Preparation	Lyophilized purified protein
Applications	Rat IgG F(c) Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Protein Description	Purified Rat IgG F(c) fragment native protein.  Rat IgG F(c) fragment was assayed by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c).   No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.

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