Bleomycin hydrolase (1-455, His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MGSSHHHHHH SSGLVPRGSH MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
Tag	His-tag
Predicted MW	54.7 kDa
Concentration	lot specific
Purity	>90%
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
Preparation	Liquid purified protein
Applications	Protocol: Activity assay 1. Prepare 100 ul of recombinant BLMH protein with various concentrations (0.06 ug, 0.03 ug) in assay buffer and equilibrate to 37°C for 30 minutes. (Assay buffer: 50 mM Tris-HCl, 5 mM EDTA, 10 mM DTT, pH 7.5) 2. Add 50 ul of 2mM Met-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Met-AMC (Enzo, Cat.No, BML-P236-0025) - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077) - Fluorescent plate reader (PerkinElmer, VICTOR X3)
Protein Description	Recombinant human BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific: > 1,000 pmole/min/ug. Measured by the hydrolysis of Met-AMC at pH 7.5, at 37°C.
Reference Data
RefSeq	NP_000377
Locus ID	642
UniProt ID	Q13867
Cytogenetics	17q11.2
Synonyms	BH; BMH
Summary	Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Protease

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